An Assay for the Quantitation of Photofrin in Tissues and Fluids
A method for determining the concentration of Photofrin in tissues and biological fluids was developed. The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement....
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| Veröffentlicht in: | Photochemistry and photobiology 1997-08, Vol.66 (2), p.237-244 |
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| container_end_page | 244 |
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| container_issue | 2 |
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| container_title | Photochemistry and photobiology |
| container_volume | 66 |
| creator | Bellnier, David A. Greco, William R. Parsons, John C. Oseroff, Allan R. Kuebler, Alex Dougherty, Thomas J. |
| description | A method for determining the concentration of Photofrin in tissues and biological fluids was developed. The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement. It was found necessary to use a quadratic standard curve for the estimation of unknown Photofrin concentrations. While this method is limited to compounds that are stable in strong base, it has the advantages of being sensitive, rapid and low cost. |
| doi_str_mv | 10.1111/j.1751-1097.1997.tb08649.x |
| format | Article |
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The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement. It was found necessary to use a quadratic standard curve for the estimation of unknown Photofrin concentrations. 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The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement. It was found necessary to use a quadratic standard curve for the estimation of unknown Photofrin concentrations. While this method is limited to compounds that are stable in strong base, it has the advantages of being sensitive, rapid and low cost.</description><subject>Animals</subject><subject>Antineoplastic Agents - analysis</subject><subject>Calibration</subject><subject>Dihematoporphyrin Ether - analysis</subject><subject>Mice</subject><subject>Reproducibility of Results</subject><subject>Solubility</subject><subject>Spectrometry, Fluorescence - methods</subject><issn>0031-8655</issn><issn>1751-1097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEFLwzAUx4Moc04_ghA8eOtMmrRpPIijuE0U3cZE8BLSNmGZXTubFrdvb0rH7oZHcvi_98vjB8ANRkPszt16iFmAPYw4G2LurjpBUUj5cHcC-sfoFPQRItiLwiA4BxfWrhHClDPcAz3uM4Yp6YPHUQFH1so91GUF65WC80YWtallbcoClhrOVmVd6soU0NXSWNsoC2WRwXHemMxegjMtc6uuDu8AfIyflvHUe32fPMejVy-ljDAv0xHhIWc8CBFK_YhrrLQiOuGUqpAkgUo4SyI_opz4DFEkQxSRFPmI0CwKFBmA2467rcoft0ItNsamKs9locrGCsZ96tC-a7zvGtOqtLZSWmwrs5HVXmAkWn1iLVpHonUkWn3ioE_s3PD14Zcm2ajsOHrw5fKHLv81udr_gyxm05lPmAN4HcDYWu2OAFl9i9B5CsTn20TM45fJ1yJeiBn5A3idjTU</recordid><startdate>199708</startdate><enddate>199708</enddate><creator>Bellnier, David A.</creator><creator>Greco, William R.</creator><creator>Parsons, John C.</creator><creator>Oseroff, Allan R.</creator><creator>Kuebler, Alex</creator><creator>Dougherty, Thomas J.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199708</creationdate><title>An Assay for the Quantitation of Photofrin in Tissues and Fluids</title><author>Bellnier, David A. ; Greco, William R. ; Parsons, John C. ; Oseroff, Allan R. ; Kuebler, Alex ; Dougherty, Thomas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4737-df83969795600c289f1efe3fb944e63b5eb97b82849327040a6083c02034d85e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - analysis</topic><topic>Calibration</topic><topic>Dihematoporphyrin Ether - analysis</topic><topic>Mice</topic><topic>Reproducibility of Results</topic><topic>Solubility</topic><topic>Spectrometry, Fluorescence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bellnier, David A.</creatorcontrib><creatorcontrib>Greco, William R.</creatorcontrib><creatorcontrib>Parsons, John C.</creatorcontrib><creatorcontrib>Oseroff, Allan R.</creatorcontrib><creatorcontrib>Kuebler, Alex</creatorcontrib><creatorcontrib>Dougherty, Thomas J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photochemistry and photobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bellnier, David A.</au><au>Greco, William R.</au><au>Parsons, John C.</au><au>Oseroff, Allan R.</au><au>Kuebler, Alex</au><au>Dougherty, Thomas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Assay for the Quantitation of Photofrin in Tissues and Fluids</atitle><jtitle>Photochemistry and photobiology</jtitle><addtitle>Photochem Photobiol</addtitle><date>1997-08</date><risdate>1997</risdate><volume>66</volume><issue>2</issue><spage>237</spage><epage>244</epage><pages>237-244</pages><issn>0031-8655</issn><eissn>1751-1097</eissn><abstract>A method for determining the concentration of Photofrin in tissues and biological fluids was developed. The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement. It was found necessary to use a quadratic standard curve for the estimation of unknown Photofrin concentrations. While this method is limited to compounds that are stable in strong base, it has the advantages of being sensitive, rapid and low cost.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9277143</pmid><doi>10.1111/j.1751-1097.1997.tb08649.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Photochemistry and photobiology, 1997-08, Vol.66 (2), p.237-244 |
| issn | 0031-8655 1751-1097 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antineoplastic Agents - analysis Calibration Dihematoporphyrin Ether - analysis Mice Reproducibility of Results Solubility Spectrometry, Fluorescence - methods |
| title | An Assay for the Quantitation of Photofrin in Tissues and Fluids |
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