Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent Reaction
The formation of dehydroluciferin (L) from luciferin (LH2) in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been studied. The E·LH2-AMP complex may follow two different pathways: towards production of light and towards the synthesis of the E·L-AMP complex. This last step has an inh...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1997-08, Vol.237 (2), p.445-450 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Fontes, Rui Dukhovich, Alexey Sillero, Antonio Sillero, Maria A.Günther |
| description | The formation of dehydroluciferin (L) from luciferin (LH2) in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been studied. The E·LH2-AMP complex may follow two different pathways: towards production of light and towards the synthesis of the E·L-AMP complex. This last step has an inhibitory effect on light emission as molecules of the enzyme are trapped in a light unproductive complex. The effects of CoA and nucleoside 5′-triphosphates (NTPs) on light emission are quantitatively different. CoA combines with the L moiety of the E·L-AMP complex, yielding L-CoA, promoting liberation of free luciferase, and increasing light yield. NTP reacts with the AMP moiety of the same complex, generating adenosine(5′)tetraphospho(5′)nucleoside (Ap4N) and, probably, the E·L complex and scarcely increasing light production. The results are discussed in relation to previous reports, by others, on luciferase. |
| doi_str_mv | 10.1006/bbrc.1997.7161 |
| format | Article |
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The E·LH2-AMP complex may follow two different pathways: towards production of light and towards the synthesis of the E·L-AMP complex. This last step has an inhibitory effect on light emission as molecules of the enzyme are trapped in a light unproductive complex. The effects of CoA and nucleoside 5′-triphosphates (NTPs) on light emission are quantitatively different. CoA combines with the L moiety of the E·L-AMP complex, yielding L-CoA, promoting liberation of free luciferase, and increasing light yield. NTP reacts with the AMP moiety of the same complex, generating adenosine(5′)tetraphospho(5′)nucleoside (Ap4N) and, probably, the E·L complex and scarcely increasing light production. The results are discussed in relation to previous reports, by others, on luciferase.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1997.7161</identifier><identifier>PMID: 9268731</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenine Nucleotides - chemistry ; Animals ; Catalysis ; Coenzyme A - chemistry ; Coleoptera - enzymology ; Firefly Luciferin - analogs & derivatives ; Firefly Luciferin - chemical synthesis ; Kinetics ; Luciferases - chemistry ; Luminescent Measurements</subject><ispartof>Biochemical and biophysical research communications, 1997-08, Vol.237 (2), p.445-450</ispartof><rights>1997 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-ae622d29a6b21394fbbaf112e92824588cc8cf7a965b102989d0044cfeb8055e3</citedby><cites>FETCH-LOGICAL-c405t-ae622d29a6b21394fbbaf112e92824588cc8cf7a965b102989d0044cfeb8055e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1997.7161$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9268731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fontes, Rui</creatorcontrib><creatorcontrib>Dukhovich, Alexey</creatorcontrib><creatorcontrib>Sillero, Antonio</creatorcontrib><creatorcontrib>Sillero, Maria A.Günther</creatorcontrib><title>Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent Reaction</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The formation of dehydroluciferin (L) from luciferin (LH2) in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been studied. 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The results are discussed in relation to previous reports, by others, on luciferase.</description><subject>Adenine Nucleotides - chemistry</subject><subject>Animals</subject><subject>Catalysis</subject><subject>Coenzyme A - chemistry</subject><subject>Coleoptera - enzymology</subject><subject>Firefly Luciferin - analogs & derivatives</subject><subject>Firefly Luciferin - chemical synthesis</subject><subject>Kinetics</subject><subject>Luciferases - chemistry</subject><subject>Luminescent Measurements</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UU1rGzEQFaEldT6uvRV06qnrSLL2Q70FN24DpoHEgdyEpB1hlV3JlXYDm9-RHxwZm56a08DMe29m3kPoMyVzSkh1pXU0cypEPa9pRU_QjBJBCkYJ_4BmJCMKJujTJ3SW0h9CKOWVOEWnglVNvaAz9Pow-WELySUcLP4B26mNoRuNsxCdx3rCKxfBdhNeH5oqwXd8Yy2Y4X-Mb3gZwL9MPeBrrHyLf4-mg5BcC3gT3W4b0m6rBsjrPM6Ls2zvPCQDfsD3oMzggr9AH63qElwe6zl6XN1slr-K9d3P2-X1ujCclEOhoGKsZUJVmtGF4FZrZSllIFjDeNk0xjTG1kpUpaaEiUa0hHBuLOiGlCUsztHXg-4uhr8jpEH2Ll_SdcpDGJOsBeMLXpMMnB-AJoaUsh9yF12v4iQpkfsY5D4GuY9B7mPIhC9H5VH30P6DH33P8-Ywh_zes4Mok3HgDbTZbTPINrj3pN8AVYOZjw</recordid><startdate>19970818</startdate><enddate>19970818</enddate><creator>Fontes, Rui</creator><creator>Dukhovich, Alexey</creator><creator>Sillero, Antonio</creator><creator>Sillero, Maria A.Günther</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970818</creationdate><title>Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent Reaction</title><author>Fontes, Rui ; Dukhovich, Alexey ; Sillero, Antonio ; Sillero, Maria A.Günther</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-ae622d29a6b21394fbbaf112e92824588cc8cf7a965b102989d0044cfeb8055e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adenine Nucleotides - chemistry</topic><topic>Animals</topic><topic>Catalysis</topic><topic>Coenzyme A - chemistry</topic><topic>Coleoptera - enzymology</topic><topic>Firefly Luciferin - analogs & derivatives</topic><topic>Firefly Luciferin - chemical synthesis</topic><topic>Kinetics</topic><topic>Luciferases - chemistry</topic><topic>Luminescent Measurements</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fontes, Rui</creatorcontrib><creatorcontrib>Dukhovich, Alexey</creatorcontrib><creatorcontrib>Sillero, Antonio</creatorcontrib><creatorcontrib>Sillero, Maria A.Günther</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fontes, Rui</au><au>Dukhovich, Alexey</au><au>Sillero, Antonio</au><au>Sillero, Maria A.Günther</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent Reaction</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1997-08-18</date><risdate>1997</risdate><volume>237</volume><issue>2</issue><spage>445</spage><epage>450</epage><pages>445-450</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The formation of dehydroluciferin (L) from luciferin (LH2) in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been studied. The E·LH2-AMP complex may follow two different pathways: towards production of light and towards the synthesis of the E·L-AMP complex. This last step has an inhibitory effect on light emission as molecules of the enzyme are trapped in a light unproductive complex. The effects of CoA and nucleoside 5′-triphosphates (NTPs) on light emission are quantitatively different. CoA combines with the L moiety of the E·L-AMP complex, yielding L-CoA, promoting liberation of free luciferase, and increasing light yield. NTP reacts with the AMP moiety of the same complex, generating adenosine(5′)tetraphospho(5′)nucleoside (Ap4N) and, probably, the E·L complex and scarcely increasing light production. The results are discussed in relation to previous reports, by others, on luciferase.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9268731</pmid><doi>10.1006/bbrc.1997.7161</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1997-08, Vol.237 (2), p.445-450 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenine Nucleotides - chemistry Animals Catalysis Coenzyme A - chemistry Coleoptera - enzymology Firefly Luciferin - analogs & derivatives Firefly Luciferin - chemical synthesis Kinetics Luciferases - chemistry Luminescent Measurements |
| title | Synthesis of Dehydroluciferin by Firefly Luciferase: Effect of Dehydroluciferin, Coenzyme A and Nucleoside Triphosphates on the Luminescent Reaction |
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