Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system
PACE4 is a mammalian Kexin family protease that is involved in the maturation of precursor proteins. Four PACE4 isoforms have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a hydrophobic cluster in its C-terminus participating in m...
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| Veröffentlicht in: | Histochemistry and cell biology 1997-08, Vol.108 (2), p.95-103 |
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| creator | Akamatsu, T Daikoku, S Nagamune, H Yoshida, S Mori, K Tsuji, A Matsuda, Y |
| description | PACE4 is a mammalian Kexin family protease that is involved in the maturation of precursor proteins. Four PACE4 isoforms have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a hydrophobic cluster in its C-terminus participating in membrane association. The size of PACE4E mRNA from adult rat brain was estimated by Northern blotting to be 4.4 kb. In situ hybridization histochemistry revealed that the highest level of PACE4E mRNA was expressed in the mitral cells of the adult rat olfactory bulb (OB). The OB is a unique sensory organ in that it has a lifelong regenerating capacity and it affects brain development. We further analyzed the expression of PACE4E mRNA in the developing olfactory system. On day 13.5 of gestation, PACE4E mRNA was expressed at high levels in the neuroepithelium of the forebrain vesicle (FV), olfactory epithelium, and cells in the fiber bundles projecting to the FV. As development proceeded, PACE4E mRNA was expressed in developing mitral cells but decreased in the olfactory epithelium. In the newborn, its expression was confined to the mitral cells in both the main and accessory OB and in some periglomerular cells, as shown in adult rats. The spatio-temporal expression of PACE4E suggests that it plays a role in the establishment and maintenance of the olfactory receptor system. |
| doi_str_mv | 10.1007/s004180050150 |
| format | Article |
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Four PACE4 isoforms have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a hydrophobic cluster in its C-terminus participating in membrane association. The size of PACE4E mRNA from adult rat brain was estimated by Northern blotting to be 4.4 kb. In situ hybridization histochemistry revealed that the highest level of PACE4E mRNA was expressed in the mitral cells of the adult rat olfactory bulb (OB). The OB is a unique sensory organ in that it has a lifelong regenerating capacity and it affects brain development. We further analyzed the expression of PACE4E mRNA in the developing olfactory system. On day 13.5 of gestation, PACE4E mRNA was expressed at high levels in the neuroepithelium of the forebrain vesicle (FV), olfactory epithelium, and cells in the fiber bundles projecting to the FV. As development proceeded, PACE4E mRNA was expressed in developing mitral cells but decreased in the olfactory epithelium. In the newborn, its expression was confined to the mitral cells in both the main and accessory OB and in some periglomerular cells, as shown in adult rats. The spatio-temporal expression of PACE4E suggests that it plays a role in the establishment and maintenance of the olfactory receptor system.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s004180050150</identifier><identifier>PMID: 9272428</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Antibody Specificity ; Blotting, Northern ; C-Terminus ; Cerebellum ; Embryonic and Fetal Development - physiology ; Female ; Forebrain ; Gene expression ; Gene Expression Regulation, Developmental - physiology ; Gene Expression Regulation, Enzymologic - physiology ; Gestation ; Humans ; Hybridization ; Hydrophobicity ; Isoforms ; Kexin ; Mitral cells ; mRNA ; Northern blotting ; Olfactory bulb ; Olfactory Bulb - embryology ; Olfactory Bulb - enzymology ; Olfactory Bulb - growth & development ; Olfactory epithelium ; Olfactory Pathways - embryology ; Olfactory Pathways - enzymology ; Olfactory Pathways - growth & development ; Olfactory system ; Proprotein Convertases ; Proteinase ; Rats ; Rats, Sprague-Dawley ; RNA, Complementary ; Rodents ; Sense organs ; Serine Endopeptidases - biosynthesis ; Serine Endopeptidases - genetics ; Smell</subject><ispartof>Histochemistry and cell biology, 1997-08, Vol.108 (2), p.95-103</ispartof><rights>Springer-Verlag Berlin Heidelberg 1997.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-e18ca07ece0a4db4a234ae68af62dcfc6cfc2cf6491069f3c68b4a523507727b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9272428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akamatsu, T</creatorcontrib><creatorcontrib>Daikoku, S</creatorcontrib><creatorcontrib>Nagamune, H</creatorcontrib><creatorcontrib>Yoshida, S</creatorcontrib><creatorcontrib>Mori, K</creatorcontrib><creatorcontrib>Tsuji, A</creatorcontrib><creatorcontrib>Matsuda, Y</creatorcontrib><title>Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><description>PACE4 is a mammalian Kexin family protease that is involved in the maturation of precursor proteins. Four PACE4 isoforms have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a hydrophobic cluster in its C-terminus participating in membrane association. The size of PACE4E mRNA from adult rat brain was estimated by Northern blotting to be 4.4 kb. In situ hybridization histochemistry revealed that the highest level of PACE4E mRNA was expressed in the mitral cells of the adult rat olfactory bulb (OB). The OB is a unique sensory organ in that it has a lifelong regenerating capacity and it affects brain development. We further analyzed the expression of PACE4E mRNA in the developing olfactory system. On day 13.5 of gestation, PACE4E mRNA was expressed at high levels in the neuroepithelium of the forebrain vesicle (FV), olfactory epithelium, and cells in the fiber bundles projecting to the FV. As development proceeded, PACE4E mRNA was expressed in developing mitral cells but decreased in the olfactory epithelium. In the newborn, its expression was confined to the mitral cells in both the main and accessory OB and in some periglomerular cells, as shown in adult rats. The spatio-temporal expression of PACE4E suggests that it plays a role in the establishment and maintenance of the olfactory receptor system.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Blotting, Northern</subject><subject>C-Terminus</subject><subject>Cerebellum</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Female</subject><subject>Forebrain</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gestation</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Hydrophobicity</subject><subject>Isoforms</subject><subject>Kexin</subject><subject>Mitral cells</subject><subject>mRNA</subject><subject>Northern blotting</subject><subject>Olfactory bulb</subject><subject>Olfactory Bulb - embryology</subject><subject>Olfactory Bulb - enzymology</subject><subject>Olfactory Bulb - growth & development</subject><subject>Olfactory epithelium</subject><subject>Olfactory Pathways - embryology</subject><subject>Olfactory Pathways - enzymology</subject><subject>Olfactory Pathways - growth & development</subject><subject>Olfactory system</subject><subject>Proprotein Convertases</subject><subject>Proteinase</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Complementary</subject><subject>Rodents</subject><subject>Sense organs</subject><subject>Serine Endopeptidases - biosynthesis</subject><subject>Serine Endopeptidases - genetics</subject><subject>Smell</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0EtLw0AQB_BFlFqrR4_CguCp0dlHXkep9YEFPSh4Mmy3s5iSZONuIs23d6VF0MMwMPNjGP6EnDK4ZADplQeQLAOIgcWwR8ZMCh4xlr_tkzHkMouSMDkkR96vIZCc8xEZ5Tzlkmdj8n6DX1jZtsamUxXFTevQ-9I21BqqaGPDlj7ipmyoUXVZDbR1tkPlcUqfr2dzOZ_SsOs-kDrVUVsZpTvrBuoH32F9TA6Mqjye7PqEvN7OX2b30eLp7mF2vYi0YEkXIcu0ghQ1gpKrpVRcSIVJpkzCV9roJBTXJpE5gyQ3QidZQDEXMaQpT5diQi62d8N3nz36rqhLr7GqVIO290WacykgEwGe_4Nr27sm_FZwCWkMHGQcVLRV2lnvHZqidWWt3FAwKH5SL_6kHvzZ7mq_rHH1q3cxi29mCHwA</recordid><startdate>19970801</startdate><enddate>19970801</enddate><creator>Akamatsu, T</creator><creator>Daikoku, S</creator><creator>Nagamune, H</creator><creator>Yoshida, S</creator><creator>Mori, K</creator><creator>Tsuji, A</creator><creator>Matsuda, Y</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>19970801</creationdate><title>Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system</title><author>Akamatsu, T ; Daikoku, S ; Nagamune, H ; Yoshida, S ; Mori, K ; Tsuji, A ; Matsuda, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-e18ca07ece0a4db4a234ae68af62dcfc6cfc2cf6491069f3c68b4a523507727b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Blotting, Northern</topic><topic>C-Terminus</topic><topic>Cerebellum</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Female</topic><topic>Forebrain</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gestation</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Hydrophobicity</topic><topic>Isoforms</topic><topic>Kexin</topic><topic>Mitral cells</topic><topic>mRNA</topic><topic>Northern blotting</topic><topic>Olfactory bulb</topic><topic>Olfactory Bulb - embryology</topic><topic>Olfactory Bulb - enzymology</topic><topic>Olfactory Bulb - growth & development</topic><topic>Olfactory epithelium</topic><topic>Olfactory Pathways - embryology</topic><topic>Olfactory Pathways - enzymology</topic><topic>Olfactory Pathways - growth & development</topic><topic>Olfactory system</topic><topic>Proprotein Convertases</topic><topic>Proteinase</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Complementary</topic><topic>Rodents</topic><topic>Sense organs</topic><topic>Serine Endopeptidases - 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Academic</collection><jtitle>Histochemistry and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akamatsu, T</au><au>Daikoku, S</au><au>Nagamune, H</au><au>Yoshida, S</au><au>Mori, K</au><au>Tsuji, A</au><au>Matsuda, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system</atitle><jtitle>Histochemistry and cell biology</jtitle><addtitle>Histochem Cell Biol</addtitle><date>1997-08-01</date><risdate>1997</risdate><volume>108</volume><issue>2</issue><spage>95</spage><epage>103</epage><pages>95-103</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>PACE4 is a mammalian Kexin family protease that is involved in the maturation of precursor proteins. Four PACE4 isoforms have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a hydrophobic cluster in its C-terminus participating in membrane association. The size of PACE4E mRNA from adult rat brain was estimated by Northern blotting to be 4.4 kb. In situ hybridization histochemistry revealed that the highest level of PACE4E mRNA was expressed in the mitral cells of the adult rat olfactory bulb (OB). The OB is a unique sensory organ in that it has a lifelong regenerating capacity and it affects brain development. We further analyzed the expression of PACE4E mRNA in the developing olfactory system. On day 13.5 of gestation, PACE4E mRNA was expressed at high levels in the neuroepithelium of the forebrain vesicle (FV), olfactory epithelium, and cells in the fiber bundles projecting to the FV. As development proceeded, PACE4E mRNA was expressed in developing mitral cells but decreased in the olfactory epithelium. In the newborn, its expression was confined to the mitral cells in both the main and accessory OB and in some periglomerular cells, as shown in adult rats. The spatio-temporal expression of PACE4E suggests that it plays a role in the establishment and maintenance of the olfactory receptor system.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>9272428</pmid><doi>10.1007/s004180050150</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0948-6143 |
| ispartof | Histochemistry and cell biology, 1997-08, Vol.108 (2), p.95-103 |
| issn | 0948-6143 1432-119X |
| language | eng |
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| subjects | Animals Antibody Specificity Blotting, Northern C-Terminus Cerebellum Embryonic and Fetal Development - physiology Female Forebrain Gene expression Gene Expression Regulation, Developmental - physiology Gene Expression Regulation, Enzymologic - physiology Gestation Humans Hybridization Hydrophobicity Isoforms Kexin Mitral cells mRNA Northern blotting Olfactory bulb Olfactory Bulb - embryology Olfactory Bulb - enzymology Olfactory Bulb - growth & development Olfactory epithelium Olfactory Pathways - embryology Olfactory Pathways - enzymology Olfactory Pathways - growth & development Olfactory system Proprotein Convertases Proteinase Rats Rats, Sprague-Dawley RNA, Complementary Rodents Sense organs Serine Endopeptidases - biosynthesis Serine Endopeptidases - genetics Smell |
| title | Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system |
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