The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells
In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1997-07, Vol.172 (1-2), p.171-194 |
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| container_title | Molecular and cellular biochemistry |
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| creator | Bkaily, G Pothier, P D'Orléans-Juste, P Simaan, M Jacques, D Jaalouk, D Belzile, F Hassan, G Boutin, C Haddad, G Neugebauer, W |
| description | In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various commercially available fluorescent probes as well as some developed in our laboratory and discuss the advantages and limitations of these probes in confocal microscopy studies of the cardiovascular system. |
| doi_str_mv | 10.1023/A:1006840228104 |
| format | Article |
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This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. 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| fulltext | fulltext |
| identifier | ISSN: 0300-8177 |
| ispartof | Molecular and cellular biochemistry, 1997-07, Vol.172 (1-2), p.171-194 |
| issn | 0300-8177 1573-4919 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Aorta Calcium - chemistry Calcium - pharmacology Cardiovascular system Cell Line Cell Nucleus - chemistry Cells Chick Embryo Cricetinae Cytosol - chemistry Endoplasmic Reticulum - metabolism Endothelin-1 - metabolism Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Endothelium, Vascular - physiology Fetus Fluorescence microscopy Fluorescent Dyes - metabolism Humans Interleukin-1 - metabolism Mice Microscopy, Confocal - methods Mitochondria, Heart - metabolism Mitochondria, Muscle - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - metabolism Muscle, Smooth, Vascular - physiology Myocardium - cytology Organelles - metabolism Platelet Activating Factor - metabolism Probes Proteins Receptor, Endothelin A Receptors, Angiotensin - metabolism Receptors, Endothelin - metabolism |
| title | The use of confocal microscopy in the investigation of cell structure and function in the heart, vascular endothelium and smooth muscle cells |
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