Peripheral blood microchimerism in human liver and renal transplant recipients : Rejection despite donor-specific chimerism
Development of donor-specific microchimerism (DSM) has been proposed as one of the possible mechanisms for induction and maintenance of allograft tolerance. The aim of this study was to determine: (1) the state of DSM in liver transplant (LTx) and renal transplant (RTx) recipients, (2) whether the p...
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| Veröffentlicht in: | Transplantation 1997-08, Vol.64 (3), p.427-432 |
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| creator | SIVASAI, K. S. R ALEVY, Y. G DUFFY, B. F BRENNAN, D. C SINGER, G. G SHENOY, S LOWELL, J. A HOWARD, T MOHANAKUMAR, T |
| description | Development of donor-specific microchimerism (DSM) has been proposed as one of the possible mechanisms for induction and maintenance of allograft tolerance. The aim of this study was to determine: (1) the state of DSM in liver transplant (LTx) and renal transplant (RTx) recipients, (2) whether the persistent presence of an allograft is a requirement for maintenance of chimerism, and (3) whether donor-specific blood transfusions (DST) facilitate chimerism development in RTx recipients and whether this correlates with allograft function.
Qualitative and quantitative analysis of DSM in peripheral blood of LTx and RTx recipients was assessed by polymerase chain reaction and competitive polymerase chain reaction using HLA-DR probes for mismatched antigens between the donor and recipient.
LTx recipients (11 of 12) who had or were having rejection were positive for DSM in circulation compared with 4 of 11 with normal allograft function (P |
| doi_str_mv | 10.1097/00007890-199708150-00010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79241008</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79241008</sourcerecordid><originalsourceid>FETCH-LOGICAL-p235t-7dec04c22c9814e73135eff76b6dc9d59c02708df50b0972553e598ca49667a23</originalsourceid><addsrcrecordid>eNo9kE1LxDAQhoMo67r6E4QcxFt1kjRN400Wv2BBET0v2WTKZmnTmnQF8c8bsOxcBuZ9eBkeQiiDGwZa3UIeVWsomNYKaiahyBcGR2TOpCiLCmo4JnOAkhVMCHVKzlLaZUQKpWZkprmSDOo5-X3D6IctRtPSTdv3jnbext5ufZeD1FEf6HbfmUBb_42RmuBoxJDpMZqQhtaEMR-sHzyGMdE7-o47tKPvA3WYBj8idX3oY5GGTDXe0kP3OTlpTJvwYtoL8vn48LF8LlavTy_L-1UxcCHHQjm0UFrOra5ZiUowIbFpVLWpnNVOags8O3CNhE12w6UUKHVtTamrShkuFuT6v3eI_dce07jufLLY5t-x36e10rxkAHUGLydwv-nQrYfoOxN_1pOtnF9NuUnWtE02YH06YFzVQoEQfxg_fDc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79241008</pqid></control><display><type>article</type><title>Peripheral blood microchimerism in human liver and renal transplant recipients : Rejection despite donor-specific chimerism</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>SIVASAI, K. S. R ; ALEVY, Y. G ; DUFFY, B. F ; BRENNAN, D. C ; SINGER, G. G ; SHENOY, S ; LOWELL, J. A ; HOWARD, T ; MOHANAKUMAR, T</creator><creatorcontrib>SIVASAI, K. S. R ; ALEVY, Y. G ; DUFFY, B. F ; BRENNAN, D. C ; SINGER, G. G ; SHENOY, S ; LOWELL, J. A ; HOWARD, T ; MOHANAKUMAR, T</creatorcontrib><description>Development of donor-specific microchimerism (DSM) has been proposed as one of the possible mechanisms for induction and maintenance of allograft tolerance. The aim of this study was to determine: (1) the state of DSM in liver transplant (LTx) and renal transplant (RTx) recipients, (2) whether the persistent presence of an allograft is a requirement for maintenance of chimerism, and (3) whether donor-specific blood transfusions (DST) facilitate chimerism development in RTx recipients and whether this correlates with allograft function.
Qualitative and quantitative analysis of DSM in peripheral blood of LTx and RTx recipients was assessed by polymerase chain reaction and competitive polymerase chain reaction using HLA-DR probes for mismatched antigens between the donor and recipient.
LTx recipients (11 of 12) who had or were having rejection were positive for DSM in circulation compared with 4 of 11 with normal allograft function (P<0.01). The number of donor cells did not correlate with allograft function. LTx recipients (4 of 4) who lost their first allograft and underwent retransplantation retained DSM for the first donors. RTx recipients who received DST (8 of 8) were positive for DSM compared with 6 of 12 of nontransfused recipients (P<0.045).
The results suggest that LTx and RTx recipients undergo rejection despite DSM. The development of DSM may not be a prerequisite for normal allograft function. Once DSM is established, the presence of the allograft is not required for maintenance of chimerism. DST facilitated the development of DSM in RTx recipients. Direct correlation was not observed between the development of DSM and allograft function in either DST or nontransfused RTx recipients.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/00007890-199708150-00010</identifier><identifier>PMID: 9275108</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Alleles ; Biological and medical sciences ; Blood Transfusion ; Blotting, Southern ; Bone Marrow Transplantation - immunology ; Graft Rejection - genetics ; Graft Rejection - pathology ; HLA-DR Antigens - genetics ; Humans ; Kidney Transplantation - immunology ; Kidney Transplantation - pathology ; Kidney Transplantation - physiology ; Liver Transplantation - immunology ; Liver Transplantation - pathology ; Liver Transplantation - physiology ; Liver, biliary tract, pancreas, portal circulation, spleen ; Medical sciences ; Polymerase Chain Reaction ; Reoperation ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the digestive system ; Transplantation Chimera - physiology ; Transplantation, Homologous - physiology</subject><ispartof>Transplantation, 1997-08, Vol.64 (3), p.427-432</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2783703$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9275108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SIVASAI, K. S. R</creatorcontrib><creatorcontrib>ALEVY, Y. G</creatorcontrib><creatorcontrib>DUFFY, B. F</creatorcontrib><creatorcontrib>BRENNAN, D. C</creatorcontrib><creatorcontrib>SINGER, G. G</creatorcontrib><creatorcontrib>SHENOY, S</creatorcontrib><creatorcontrib>LOWELL, J. A</creatorcontrib><creatorcontrib>HOWARD, T</creatorcontrib><creatorcontrib>MOHANAKUMAR, T</creatorcontrib><title>Peripheral blood microchimerism in human liver and renal transplant recipients : Rejection despite donor-specific chimerism</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Development of donor-specific microchimerism (DSM) has been proposed as one of the possible mechanisms for induction and maintenance of allograft tolerance. The aim of this study was to determine: (1) the state of DSM in liver transplant (LTx) and renal transplant (RTx) recipients, (2) whether the persistent presence of an allograft is a requirement for maintenance of chimerism, and (3) whether donor-specific blood transfusions (DST) facilitate chimerism development in RTx recipients and whether this correlates with allograft function.
Qualitative and quantitative analysis of DSM in peripheral blood of LTx and RTx recipients was assessed by polymerase chain reaction and competitive polymerase chain reaction using HLA-DR probes for mismatched antigens between the donor and recipient.
LTx recipients (11 of 12) who had or were having rejection were positive for DSM in circulation compared with 4 of 11 with normal allograft function (P<0.01). The number of donor cells did not correlate with allograft function. LTx recipients (4 of 4) who lost their first allograft and underwent retransplantation retained DSM for the first donors. RTx recipients who received DST (8 of 8) were positive for DSM compared with 6 of 12 of nontransfused recipients (P<0.045).
The results suggest that LTx and RTx recipients undergo rejection despite DSM. The development of DSM may not be a prerequisite for normal allograft function. Once DSM is established, the presence of the allograft is not required for maintenance of chimerism. DST facilitated the development of DSM in RTx recipients. Direct correlation was not observed between the development of DSM and allograft function in either DST or nontransfused RTx recipients.</description><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>Blood Transfusion</subject><subject>Blotting, Southern</subject><subject>Bone Marrow Transplantation - immunology</subject><subject>Graft Rejection - genetics</subject><subject>Graft Rejection - pathology</subject><subject>HLA-DR Antigens - genetics</subject><subject>Humans</subject><subject>Kidney Transplantation - immunology</subject><subject>Kidney Transplantation - pathology</subject><subject>Kidney Transplantation - physiology</subject><subject>Liver Transplantation - immunology</subject><subject>Liver Transplantation - pathology</subject><subject>Liver Transplantation - physiology</subject><subject>Liver, biliary tract, pancreas, portal circulation, spleen</subject><subject>Medical sciences</subject><subject>Polymerase Chain Reaction</subject><subject>Reoperation</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the digestive system</subject><subject>Transplantation Chimera - physiology</subject><subject>Transplantation, Homologous - physiology</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo67r6E4QcxFt1kjRN400Wv2BBET0v2WTKZmnTmnQF8c8bsOxcBuZ9eBkeQiiDGwZa3UIeVWsomNYKaiahyBcGR2TOpCiLCmo4JnOAkhVMCHVKzlLaZUQKpWZkprmSDOo5-X3D6IctRtPSTdv3jnbext5ufZeD1FEf6HbfmUBb_42RmuBoxJDpMZqQhtaEMR-sHzyGMdE7-o47tKPvA3WYBj8idX3oY5GGTDXe0kP3OTlpTJvwYtoL8vn48LF8LlavTy_L-1UxcCHHQjm0UFrOra5ZiUowIbFpVLWpnNVOags8O3CNhE12w6UUKHVtTamrShkuFuT6v3eI_dce07jufLLY5t-x36e10rxkAHUGLydwv-nQrYfoOxN_1pOtnF9NuUnWtE02YH06YFzVQoEQfxg_fDc</recordid><startdate>19970815</startdate><enddate>19970815</enddate><creator>SIVASAI, K. S. R</creator><creator>ALEVY, Y. G</creator><creator>DUFFY, B. F</creator><creator>BRENNAN, D. C</creator><creator>SINGER, G. G</creator><creator>SHENOY, S</creator><creator>LOWELL, J. A</creator><creator>HOWARD, T</creator><creator>MOHANAKUMAR, T</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970815</creationdate><title>Peripheral blood microchimerism in human liver and renal transplant recipients : Rejection despite donor-specific chimerism</title><author>SIVASAI, K. S. R ; ALEVY, Y. G ; DUFFY, B. F ; BRENNAN, D. C ; SINGER, G. G ; SHENOY, S ; LOWELL, J. A ; HOWARD, T ; MOHANAKUMAR, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-7dec04c22c9814e73135eff76b6dc9d59c02708df50b0972553e598ca49667a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Alleles</topic><topic>Biological and medical sciences</topic><topic>Blood Transfusion</topic><topic>Blotting, Southern</topic><topic>Bone Marrow Transplantation - immunology</topic><topic>Graft Rejection - genetics</topic><topic>Graft Rejection - pathology</topic><topic>HLA-DR Antigens - genetics</topic><topic>Humans</topic><topic>Kidney Transplantation - immunology</topic><topic>Kidney Transplantation - pathology</topic><topic>Kidney Transplantation - physiology</topic><topic>Liver Transplantation - immunology</topic><topic>Liver Transplantation - pathology</topic><topic>Liver Transplantation - physiology</topic><topic>Liver, biliary tract, pancreas, portal circulation, spleen</topic><topic>Medical sciences</topic><topic>Polymerase Chain Reaction</topic><topic>Reoperation</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the digestive system</topic><topic>Transplantation Chimera - physiology</topic><topic>Transplantation, Homologous - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SIVASAI, K. S. R</creatorcontrib><creatorcontrib>ALEVY, Y. G</creatorcontrib><creatorcontrib>DUFFY, B. F</creatorcontrib><creatorcontrib>BRENNAN, D. C</creatorcontrib><creatorcontrib>SINGER, G. G</creatorcontrib><creatorcontrib>SHENOY, S</creatorcontrib><creatorcontrib>LOWELL, J. A</creatorcontrib><creatorcontrib>HOWARD, T</creatorcontrib><creatorcontrib>MOHANAKUMAR, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SIVASAI, K. S. R</au><au>ALEVY, Y. G</au><au>DUFFY, B. F</au><au>BRENNAN, D. C</au><au>SINGER, G. G</au><au>SHENOY, S</au><au>LOWELL, J. A</au><au>HOWARD, T</au><au>MOHANAKUMAR, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peripheral blood microchimerism in human liver and renal transplant recipients : Rejection despite donor-specific chimerism</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>1997-08-15</date><risdate>1997</risdate><volume>64</volume><issue>3</issue><spage>427</spage><epage>432</epage><pages>427-432</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Development of donor-specific microchimerism (DSM) has been proposed as one of the possible mechanisms for induction and maintenance of allograft tolerance. The aim of this study was to determine: (1) the state of DSM in liver transplant (LTx) and renal transplant (RTx) recipients, (2) whether the persistent presence of an allograft is a requirement for maintenance of chimerism, and (3) whether donor-specific blood transfusions (DST) facilitate chimerism development in RTx recipients and whether this correlates with allograft function.
Qualitative and quantitative analysis of DSM in peripheral blood of LTx and RTx recipients was assessed by polymerase chain reaction and competitive polymerase chain reaction using HLA-DR probes for mismatched antigens between the donor and recipient.
LTx recipients (11 of 12) who had or were having rejection were positive for DSM in circulation compared with 4 of 11 with normal allograft function (P<0.01). The number of donor cells did not correlate with allograft function. LTx recipients (4 of 4) who lost their first allograft and underwent retransplantation retained DSM for the first donors. RTx recipients who received DST (8 of 8) were positive for DSM compared with 6 of 12 of nontransfused recipients (P<0.045).
The results suggest that LTx and RTx recipients undergo rejection despite DSM. The development of DSM may not be a prerequisite for normal allograft function. Once DSM is established, the presence of the allograft is not required for maintenance of chimerism. DST facilitated the development of DSM in RTx recipients. Direct correlation was not observed between the development of DSM and allograft function in either DST or nontransfused RTx recipients.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>9275108</pmid><doi>10.1097/00007890-199708150-00010</doi><tpages>6</tpages></addata></record> |
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| ispartof | Transplantation, 1997-08, Vol.64 (3), p.427-432 |
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| subjects | Alleles Biological and medical sciences Blood Transfusion Blotting, Southern Bone Marrow Transplantation - immunology Graft Rejection - genetics Graft Rejection - pathology HLA-DR Antigens - genetics Humans Kidney Transplantation - immunology Kidney Transplantation - pathology Kidney Transplantation - physiology Liver Transplantation - immunology Liver Transplantation - pathology Liver Transplantation - physiology Liver, biliary tract, pancreas, portal circulation, spleen Medical sciences Polymerase Chain Reaction Reoperation Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the digestive system Transplantation Chimera - physiology Transplantation, Homologous - physiology |
| title | Peripheral blood microchimerism in human liver and renal transplant recipients : Rejection despite donor-specific chimerism |
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