The application of immunoblotting to the phenotyping of Group-specific component
A sensitive immunoblotting method for the routine detection of group-specific component (GC) from fresh serum, and from control and casework bloodstains has been developed. GC phenotypes were separated in a thin layer polyacrylamide gel by isoelectric focusing, transferred to nitrocellulose by a rap...
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| Veröffentlicht in: | Journal - Forensic Science Society 1989-05, Vol.29 (3), p.197-205 |
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| container_title | Journal - Forensic Science Society |
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| creator | Thomas, A.S. Ansford, A.J. Aaskov, J.G. |
| description | A sensitive immunoblotting method for the routine detection of group-specific component (GC) from fresh serum, and from control and casework bloodstains has been developed. GC phenotypes were separated in a thin layer polyacrylamide gel by isoelectric focusing, transferred to nitrocellulose by a rapid capillary blotting procedure, and detected using a double antibody enzyme immunoassay. This method is capable of phenotyping 8 ng of GC extracted from bloodstains, a fourfold increase in sensitivity when compared to immunofixation and silverstaining. A total of 2424 casework bloodstains have been analysed and GC phenotypes identified in 78% of samples. The method is suitable for use in routine laboratories and is more sensitive than other methods for GC phenotyping of casework bloodstains. |
| doi_str_mv | 10.1016/S0015-7368(89)73248-5 |
| format | Article |
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GC phenotypes were separated in a thin layer polyacrylamide gel by isoelectric focusing, transferred to nitrocellulose by a rapid capillary blotting procedure, and detected using a double antibody enzyme immunoassay. This method is capable of phenotyping 8 ng of GC extracted from bloodstains, a fourfold increase in sensitivity when compared to immunofixation and silverstaining. A total of 2424 casework bloodstains have been analysed and GC phenotypes identified in 78% of samples. 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GC phenotypes were separated in a thin layer polyacrylamide gel by isoelectric focusing, transferred to nitrocellulose by a rapid capillary blotting procedure, and detected using a double antibody enzyme immunoassay. This method is capable of phenotyping 8 ng of GC extracted from bloodstains, a fourfold increase in sensitivity when compared to immunofixation and silverstaining. A total of 2424 casework bloodstains have been analysed and GC phenotypes identified in 78% of samples. The method is suitable for use in routine laboratories and is more sensitive than other methods for GC phenotyping of casework bloodstains.</description><subject>Blood Stains</subject><subject>Bloodstains</subject><subject>Group-specific component</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Phenotype</subject><subject>Vitamin D-Binding Protein - blood</subject><subject>Vitamin D-Binding Protein - genetics</subject><issn>0015-7368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQx3NQ1vXxEYSeRA_VSds07UlEdBUEBfUc0unUjbRNbFJhv73dB3v1NPB_zDA_xs45XHPg-c07ABexTPPisiivZJpkRSwO2HwvH7Fj778BcshlOmOzRJZZydM5e_tYUqSdaw3qYGwf2SYyXTf2tmptCKb_ioKNwhRyS-ptWLm1NIUWgx1d7B2haQxGaDtne-rDKTtsdOvpbDdP2Ofjw8f9U_zyuni-v3uJMc0hxEkmMUGhG9QcpW6yIoO0gjqTWY5Qcch0Ao0AElwD8YY0SSgFVrIgklinJ-xiu9cN9mckH1RnPFLb6p7s6JUskzQXIpmCYhvEwXo_UKPcYDo9rBQHtaanNvTUGpMqSrWhp8TUO98dGKuO6n1rh27yb7c-TV_-GhqUR0M9Um0GwqBqa_658Afw9oKn</recordid><startdate>19890501</startdate><enddate>19890501</enddate><creator>Thomas, A.S.</creator><creator>Ansford, A.J.</creator><creator>Aaskov, J.G.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890501</creationdate><title>The application of immunoblotting to the phenotyping of Group-specific component</title><author>Thomas, A.S. ; Ansford, A.J. ; Aaskov, J.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-247c2c5afca1c7af48403b0d4746c0b104a20f50e51a0e1feae7095cb78ee7cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Blood Stains</topic><topic>Bloodstains</topic><topic>Group-specific component</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Phenotype</topic><topic>Vitamin D-Binding Protein - blood</topic><topic>Vitamin D-Binding Protein - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Thomas, A.S.</creatorcontrib><creatorcontrib>Ansford, A.J.</creatorcontrib><creatorcontrib>Aaskov, J.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal - Forensic Science Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, A.S.</au><au>Ansford, A.J.</au><au>Aaskov, J.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The application of immunoblotting to the phenotyping of Group-specific component</atitle><jtitle>Journal - Forensic Science Society</jtitle><addtitle>J Forensic Sci Soc</addtitle><date>1989-05-01</date><risdate>1989</risdate><volume>29</volume><issue>3</issue><spage>197</spage><epage>205</epage><pages>197-205</pages><issn>0015-7368</issn><abstract>A sensitive immunoblotting method for the routine detection of group-specific component (GC) from fresh serum, and from control and casework bloodstains has been developed. GC phenotypes were separated in a thin layer polyacrylamide gel by isoelectric focusing, transferred to nitrocellulose by a rapid capillary blotting procedure, and detected using a double antibody enzyme immunoassay. This method is capable of phenotyping 8 ng of GC extracted from bloodstains, a fourfold increase in sensitivity when compared to immunofixation and silverstaining. A total of 2424 casework bloodstains have been analysed and GC phenotypes identified in 78% of samples. The method is suitable for use in routine laboratories and is more sensitive than other methods for GC phenotyping of casework bloodstains.</abstract><cop>England</cop><pub>Elsevier Ireland Ltd</pub><pmid>2794913</pmid><doi>10.1016/S0015-7368(89)73248-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal - Forensic Science Society, 1989-05, Vol.29 (3), p.197-205 |
| issn | 0015-7368 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Blood Stains Bloodstains Group-specific component Humans Immunoblotting Phenotype Vitamin D-Binding Protein - blood Vitamin D-Binding Protein - genetics |
| title | The application of immunoblotting to the phenotyping of Group-specific component |
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