Whole-blood lymphocyte cultures

A simple and reproducible method is desribed for the measurement of proliferative responses of peripheral blood lymphocytes in whole blood upon stimulation with horse anti-human lymphocyte serum (ALS), phytohaemagglutinin (PHA) or a monoclonal antibody (mAb) directed against CD3. Only small aliquots...

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Veröffentlicht in:	Journal of immunological methods 1989-09, Vol.122 (2), p.161-167
Hauptverfasser:	Bloemena, E., Roos, M.T.L., Van Heijst, J.L.A.M., Vossen, J.M.J.J., Schellekens, P.T.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	aCD3-induced proliferation Adolescent Adult Aged Animal cells Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte - immunology Biological and medical sciences Biotechnology CD3 Complex Cells, Cultured Eukaryotic cell cultures Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunologic Deficiency Syndromes - immunology Lymphocyte Activation Male Methods. Procedures. Technologies Middle Aged Miscellaneous Molecular immunology Receptors, Antigen, T-Cell - immunology Reference value Reference Values T-Lymphocytes - immunology Techniques Whole-blood lymphocyte culture
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