Immunohistochemical processing of tissue which allows for embedding in Araldite®, and orientation and correlation between light and electron microscopic staining
This paper describes a simple and efficient method for the immmunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite® medium. These sections present in one plane can then be orientated and when cut provide the user with whole...
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| Veröffentlicht in: | Journal of microscopy (Oxford) 1997-07, Vol.187 (1), p.68-72 |
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| container_end_page | 72 |
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| container_title | Journal of microscopy (Oxford) |
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| creator | Oates, P. S. Light, A. Thomas, C. |
| description | This paper describes a simple and efficient method for the immmunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite® medium. These sections present in one plane can then be orientated and when cut provide the user with whole tissue, free from gaps due to folding. This procedure therefore enables the changes in protein concentration and distribution to be assessed and correlated within and between different cells at the light and electron microscopic level. This procedure may be of use in other microscopic techniques studies. |
| doi_str_mv | 10.1046/j.1365-2818.1997.2110775.x |
| format | Article |
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This procedure may be of use in other microscopic techniques studies.</description><identifier>ISSN: 0022-2720</identifier><identifier>EISSN: 1365-2818</identifier><identifier>DOI: 10.1046/j.1365-2818.1997.2110775.x</identifier><identifier>PMID: 9263440</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Correlation ; embedding ; Epoxy Resins ; examination ; immunohistochemistry ; Immunohistochemistry - methods ; light microscopy ; Male ; Microscopy, Electron ; orientation ; Phthalic Anhydrides ; Rats ; Rats, Wistar ; Staining and Labeling ; TEM ; tissue ; Tissue Embedding</subject><ispartof>Journal of microscopy (Oxford), 1997-07, Vol.187 (1), p.68-72</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3691-c238c2eb26bd4710feb4dcbb0faf6595d8f54aa9d25546c04803757a0029f3f63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2818.1997.2110775.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2818.1997.2110775.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9263440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oates, P. S.</creatorcontrib><creatorcontrib>Light, A.</creatorcontrib><creatorcontrib>Thomas, C.</creatorcontrib><title>Immunohistochemical processing of tissue which allows for embedding in Araldite®, and orientation and correlation between light and electron microscopic staining</title><title>Journal of microscopy (Oxford)</title><addtitle>J Microsc</addtitle><description>This paper describes a simple and efficient method for the immmunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite® medium. These sections present in one plane can then be orientated and when cut provide the user with whole tissue, free from gaps due to folding. This procedure therefore enables the changes in protein concentration and distribution to be assessed and correlated within and between different cells at the light and electron microscopic level. This procedure may be of use in other microscopic techniques studies.</description><subject>Animals</subject><subject>Correlation</subject><subject>embedding</subject><subject>Epoxy Resins</subject><subject>examination</subject><subject>immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>light microscopy</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>orientation</subject><subject>Phthalic Anhydrides</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Staining and Labeling</subject><subject>TEM</subject><subject>tissue</subject><subject>Tissue Embedding</subject><issn>0022-2720</issn><issn>1365-2818</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUFu1DAYhS0EKkPhCEgWC1ZNsJ3YSVggVRW0g4rYwNpynN-NR0482I6mvQ4H4BCcDKcz6p6V9fTe_37bH0LvKCkpqcWHXUkrwQvW0rakXdeUjFLSNLy8f4Y2T9ZztCGEsYI1jLxEr2LcEUJa3pIzdNYxUdU12aDf22laZj_amLweYbJaObwPXkOMdr7D3uBkY1wAH0arR6yc84eIjQ8Yph6GYQ3ZGV8G5Qab4O-fC6zmAftgYU4qWT8_au1DAHfUPaQDwIydvRvTowsOdArZyvuDj9rvrcYxKTvn-tfohVEuwpvTeY5-fvn84-qmuP1-vb26vC10JTpaaFa1mkHPRD_UDSUG-nrQfU-MMoJ3fGgNr5XqBsZ5LTSpW1I1vFH5jzpTGVGdo_fH3vz8XwvEJCcbNTinZvBLlE3HGBOC5-DHY3C9awxg5D7YSYUHSYlcAcmdXCnIlYJcAckTIHmfh9-etiz9BMPT6IlI9j8d_YN18PAfzfLrt20W1T8SQ6TV</recordid><startdate>199707</startdate><enddate>199707</enddate><creator>Oates, P. S.</creator><creator>Light, A.</creator><creator>Thomas, C.</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199707</creationdate><title>Immunohistochemical processing of tissue which allows for embedding in Araldite®, and orientation and correlation between light and electron microscopic staining</title><author>Oates, P. S. ; Light, A. ; Thomas, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3691-c238c2eb26bd4710feb4dcbb0faf6595d8f54aa9d25546c04803757a0029f3f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Correlation</topic><topic>embedding</topic><topic>Epoxy Resins</topic><topic>examination</topic><topic>immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>light microscopy</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>orientation</topic><topic>Phthalic Anhydrides</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Staining and Labeling</topic><topic>TEM</topic><topic>tissue</topic><topic>Tissue Embedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oates, P. S.</creatorcontrib><creatorcontrib>Light, A.</creatorcontrib><creatorcontrib>Thomas, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microscopy (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oates, P. S.</au><au>Light, A.</au><au>Thomas, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical processing of tissue which allows for embedding in Araldite®, and orientation and correlation between light and electron microscopic staining</atitle><jtitle>Journal of microscopy (Oxford)</jtitle><addtitle>J Microsc</addtitle><date>1997-07</date><risdate>1997</risdate><volume>187</volume><issue>1</issue><spage>68</spage><epage>72</epage><pages>68-72</pages><issn>0022-2720</issn><eissn>1365-2818</eissn><abstract>This paper describes a simple and efficient method for the immmunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite® medium. These sections present in one plane can then be orientated and when cut provide the user with whole tissue, free from gaps due to folding. This procedure therefore enables the changes in protein concentration and distribution to be assessed and correlated within and between different cells at the light and electron microscopic level. This procedure may be of use in other microscopic techniques studies.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9263440</pmid><doi>10.1046/j.1365-2818.1997.2110775.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of microscopy (Oxford), 1997-07, Vol.187 (1), p.68-72 |
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| language | eng |
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| source | MEDLINE; Wiley Free Content; Wiley Online Library All Journals |
| subjects | Animals Correlation embedding Epoxy Resins examination immunohistochemistry Immunohistochemistry - methods light microscopy Male Microscopy, Electron orientation Phthalic Anhydrides Rats Rats, Wistar Staining and Labeling TEM tissue Tissue Embedding |
| title | Immunohistochemical processing of tissue which allows for embedding in Araldite®, and orientation and correlation between light and electron microscopic staining |
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