Bis(sulfo-N-succinimidyl) doxyl-2-spiro-5'-azelate: synthesis, characterization, and reaction with the anion-exchange channel in intact human erythrocytes

We have synthesized and characterized bis(sulfo-N-succinimidyl) doxyl-2-spiro-5'-azelate (BSSDA), a membrane-impermeant bifunctional spin-labeling reagent. BSSDA is a nine carbon backbone homologue of bis(sulfo-N-succinimidyl) doxyl-2-spiro-4'-pimelate [BSSDP; Beth et al. (1986) Biochemist...

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Veröffentlicht in:	Biochemistry (Easton) 1989-08, Vol.28 (16), p.6583-6590
Hauptverfasser:	Anjaneyulu, P. S. R, Beth, Albert H, Cobb, Charles E, Juliao, Saul F, Sweetman, Brian J, Staros, James V
Format:	Artikel
Sprache:	eng
Schlagworte:	4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid Anions Biological and medical sciences bis(sulfo-N-succinimidyl) doxyl-2-spiro-5'-azelate Conformational dynamics in molecular biology Cross-Linking Reagents - chemical synthesis Electron Spin Resonance Spectroscopy erythrocytes Erythrocytes - metabolism Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Ion Channels - metabolism Ion Exchange man Molecular biophysics Oxazoles - chemical synthesis Oxazoles - metabolism Spin Labels - chemical synthesis Succinimides - chemical synthesis Succinimides - metabolism
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