Anti-biotin Antibodies Offer Superior Organelle-specific Labeling of Mitochondria over Avidin or Streptavidin

The mitochondrial matrix contains endogenously biotinylated proteins. These proteins can cause unexpected background signal when biotin–avidin- or biotin–streptavidin-based detection systems are used in immunocytochemistry. Here we show that this reactivity can be deliberately exploited, using a sim...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 1997-08, Vol.45 (8), p.1053-1057
Hauptverfasser:	Hollinshead, Michael, Sanderson, Jeremy, Vaux, David J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Avidin - analysis Bacterial Proteins - analysis Biotin - analysis Biotin - immunology Cells, Cultured Kidney - chemistry Kidney - ultrastructure Microscopy, Electron Microscopy, Fluorescence Microscopy, Immunoelectron Mitochondria - chemistry Mitochondria - ultrastructure Rats Streptavidin
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