HPLC determination of biologically active thiols using pre-column derivatisation with 5,5′-dithio-(bis-2-nitrobenzoic acid)

5,5′-Dithio-(bis-2-nitrobenzoic acid), Ellmans reagent (ESSE), is used as a pre-column derivatisation reagent for the determination of biologically active thiols by HPLC. d-penicillamine, N-acetyl- d-penicillamine, N-acetylcysteine, cysteine, captopril and thiomalic acid all give well resolved deriv...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 1997-07, Vol.15 (11), p.1757-1763
Hauptverfasser:	Russell, J, McKeown, J.A, Hensman, C, Smith, W.E, Reglinski, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biological and medical sciences Chromatography, High Pressure Liquid Dithionitrobenzoic Acid Ellmans reagent General pharmacology HPLC Medical sciences Pharmacology. Drug treatments Pre-column derivatisation Sulfhydryl Compounds - analysis Thiol assay
Online-Zugang:	Volltext
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