Influence of the valency and hydrophobicity of an antigen on its efficiency of processing and presentation by antigen-specific B cells
We examined the influence of antigen structure on the efficiency of antigen-specific B-cell processing and presentation. The ability of untransfected and anti-TNP surface IgM (sIgM) bearing transfected TA3 B-hybridoma cells to process and present various conjugates of 2,4,6-trinitrophenyl-pork insul...
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| Veröffentlicht in: | Research in immunology (Paris) 1989-01, Vol.140 (5), p.563-579 |
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| creator | Bernard, N.F Naquet, P Watanabe, M Hozumi, N Delovitch, T.L |
| description | We examined the influence of antigen structure on the efficiency of antigen-specific B-cell processing and presentation. The ability of untransfected and anti-TNP surface IgM (sIgM) bearing transfected TA3 B-hybridoma cells to process and present various conjugates of 2,4,6-trinitrophenyl-pork insulin (TNP-PI) to PI/I-A
d-specific T cells was investigated. Similar antigen concentrations were required for presentation of underivatized antigen by untransfected and transfected cells (antigen-non-specific presentation). Transfected cells present TNP-PI at about a 50-fold lower concentration and TNP-ovalbumin (TNP-OVA) at a 600-fold lower concentration than do untransfected cells (antigen-specific presentation). PI was derivatized at 3 possible residues, A1, B1 and B29, and the different TNP-PI conjugates obtained were separated by hydrophobic interaction high-pressure liquid chromatography. All TNP
1-PI conjugates were presented equally by transfected and untransfected cells. Transfected TA3 cells presented a TNP
2-PI conjugate derivatized at A1 and B29, TNP
2(A1, B29)-PI, at about a 50-fold lower concentration than was required by untransfected cells. Another TNP
2-PI conjugate, derivatized at residues A1 and B1, TNP
2(A1, B1)-PI, and a TNP
3(A1,B1,B29)-PI conjugate were presented at about the same concentration by transfected
vs untransfected B cells. Of the various TNP conjugates tested, only TNP
2(A1,B29)-PI, which is more hydrophobic than any of the other TNP-PI conjugates, was processed more efficiently by transfected anti-TNP-specific TA3 cells
vs untransfected cells. The efficiency of presentation of these TNP-PI conjugates was directly proportional to their rate of processing. Our data demonstrate that the valency and relative hydrophobicity of an antigen control its binding to sIgM and the efficiency of processing and presentation by B cells.
Nous avons évalué l'influence de la structure d'un antigène sur sa capacité d'être métabolisé et présenté par des lymphocytes B. Nous avons comparé la présentation de l'insuline de porc couplée au TNP (TNP-PI) à des hybridomes T spécifiques de PI dans le contexte I-A
d par des cellules TA3 parentales ou transfectées avec le gène codant pour une IgM membranaire spécifique du TNP. Alors que les concentrations antigéniques requises pour activer les cellules T étaient comparables en l'absence de TNP (présentation non spécifique de l'antigène), il devenait possible de les diminuer de l'ordr de 50 fois pour TNP-PI et |
| doi_str_mv | 10.1016/0923-2494(89)90120-X |
| format | Article |
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d-specific T cells was investigated. Similar antigen concentrations were required for presentation of underivatized antigen by untransfected and transfected cells (antigen-non-specific presentation). Transfected cells present TNP-PI at about a 50-fold lower concentration and TNP-ovalbumin (TNP-OVA) at a 600-fold lower concentration than do untransfected cells (antigen-specific presentation). PI was derivatized at 3 possible residues, A1, B1 and B29, and the different TNP-PI conjugates obtained were separated by hydrophobic interaction high-pressure liquid chromatography. All TNP
1-PI conjugates were presented equally by transfected and untransfected cells. Transfected TA3 cells presented a TNP
2-PI conjugate derivatized at A1 and B29, TNP
2(A1, B29)-PI, at about a 50-fold lower concentration than was required by untransfected cells. Another TNP
2-PI conjugate, derivatized at residues A1 and B1, TNP
2(A1, B1)-PI, and a TNP
3(A1,B1,B29)-PI conjugate were presented at about the same concentration by transfected
vs untransfected B cells. Of the various TNP conjugates tested, only TNP
2(A1,B29)-PI, which is more hydrophobic than any of the other TNP-PI conjugates, was processed more efficiently by transfected anti-TNP-specific TA3 cells
vs untransfected cells. The efficiency of presentation of these TNP-PI conjugates was directly proportional to their rate of processing. Our data demonstrate that the valency and relative hydrophobicity of an antigen control its binding to sIgM and the efficiency of processing and presentation by B cells.
Nous avons évalué l'influence de la structure d'un antigène sur sa capacité d'être métabolisé et présenté par des lymphocytes B. Nous avons comparé la présentation de l'insuline de porc couplée au TNP (TNP-PI) à des hybridomes T spécifiques de PI dans le contexte I-A
d par des cellules TA3 parentales ou transfectées avec le gène codant pour une IgM membranaire spécifique du TNP. Alors que les concentrations antigéniques requises pour activer les cellules T étaient comparables en l'absence de TNP (présentation non spécifique de l'antigène), il devenait possible de les diminuer de l'ordr de 50 fois pour TNP-PI et 600 fois pour TNP-OVA en utilisant les cellules les B transfectées (présentation spécique de l'antigène). Les conjugués TNP-PI sont potentiellement modifies sur 3 résidus, A1, B1 et B29, et peuvent être séparés par chromatographie hydrophobe. La présentation par les cellules B spécifiques est inchangée pour le dérivé TNP
1-PI alors qu'elle est 50 fois amplifiée pour le dérivé TNP
2-PI en A1 et B29 (TNP
2(A1,B29)-PI); par contre, les conjugués (TNP
2(A1,B1)-PI) et (TNP
3(A1,B1,B29)-PI) sont présentés de façon comparable sur cellules transfectées ou non. Ainsi seul le conjugué le plus hydrophobe (TNP
2(A1,B29)-PI) subit une présentation plus efficace par des cellules B spécifiques. L'efficacité de présentation est directement proportionnelle au taux de métabolisation. Nos résultats démontrent que la valence et l'hydrophobicité d'un antigène déterminent son interaction avec des IgM membranaires et l'efficacité de présentation par des lymphocytes B.</description><identifier>ISSN: 0923-2494</identifier><identifier>DOI: 10.1016/0923-2494(89)90120-X</identifier><identifier>PMID: 2675228</identifier><language>eng</language><publisher>France: Elsevier B.V</publisher><subject>Animals ; Antigen-Presenting Cells - immunology ; Antigens ; B lymphocyte, Valency, Hydrophobicity, Antigen binding, sIgM ; B-cell processing and presentation, T cell, Derivatization, Pork insulin, TNP conjugation ; B-Lymphocytes - immunology ; Genes, Immunoglobulin ; Haptens ; Insulin - immunology ; Lymphocyte B, Valence, Hydrophobicité, Liaison antigénique, sIgM ; Métabolisation et présentation des cellules B, Lymphocyte T, Dérivation, Insuline de porc, TNP ; Ovalbumin - immunology ; Transfection ; Trinitrobenzenes - immunology</subject><ispartof>Research in immunology (Paris), 1989-01, Vol.140 (5), p.563-579</ispartof><rights>1989</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-1a6365ab0a43676cf0802c0f71caaf55012e213de2c2bf8ab1467e5b885f3ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2675228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bernard, N.F</creatorcontrib><creatorcontrib>Naquet, P</creatorcontrib><creatorcontrib>Watanabe, M</creatorcontrib><creatorcontrib>Hozumi, N</creatorcontrib><creatorcontrib>Delovitch, T.L</creatorcontrib><title>Influence of the valency and hydrophobicity of an antigen on its efficiency of processing and presentation by antigen-specific B cells</title><title>Research in immunology (Paris)</title><addtitle>Res Immunol</addtitle><description>We examined the influence of antigen structure on the efficiency of antigen-specific B-cell processing and presentation. The ability of untransfected and anti-TNP surface IgM (sIgM) bearing transfected TA3 B-hybridoma cells to process and present various conjugates of 2,4,6-trinitrophenyl-pork insulin (TNP-PI) to PI/I-A
d-specific T cells was investigated. Similar antigen concentrations were required for presentation of underivatized antigen by untransfected and transfected cells (antigen-non-specific presentation). Transfected cells present TNP-PI at about a 50-fold lower concentration and TNP-ovalbumin (TNP-OVA) at a 600-fold lower concentration than do untransfected cells (antigen-specific presentation). PI was derivatized at 3 possible residues, A1, B1 and B29, and the different TNP-PI conjugates obtained were separated by hydrophobic interaction high-pressure liquid chromatography. All TNP
1-PI conjugates were presented equally by transfected and untransfected cells. Transfected TA3 cells presented a TNP
2-PI conjugate derivatized at A1 and B29, TNP
2(A1, B29)-PI, at about a 50-fold lower concentration than was required by untransfected cells. Another TNP
2-PI conjugate, derivatized at residues A1 and B1, TNP
2(A1, B1)-PI, and a TNP
3(A1,B1,B29)-PI conjugate were presented at about the same concentration by transfected
vs untransfected B cells. Of the various TNP conjugates tested, only TNP
2(A1,B29)-PI, which is more hydrophobic than any of the other TNP-PI conjugates, was processed more efficiently by transfected anti-TNP-specific TA3 cells
vs untransfected cells. The efficiency of presentation of these TNP-PI conjugates was directly proportional to their rate of processing. Our data demonstrate that the valency and relative hydrophobicity of an antigen control its binding to sIgM and the efficiency of processing and presentation by B cells.
Nous avons évalué l'influence de la structure d'un antigène sur sa capacité d'être métabolisé et présenté par des lymphocytes B. Nous avons comparé la présentation de l'insuline de porc couplée au TNP (TNP-PI) à des hybridomes T spécifiques de PI dans le contexte I-A
d par des cellules TA3 parentales ou transfectées avec le gène codant pour une IgM membranaire spécifique du TNP. Alors que les concentrations antigéniques requises pour activer les cellules T étaient comparables en l'absence de TNP (présentation non spécifique de l'antigène), il devenait possible de les diminuer de l'ordr de 50 fois pour TNP-PI et 600 fois pour TNP-OVA en utilisant les cellules les B transfectées (présentation spécique de l'antigène). Les conjugués TNP-PI sont potentiellement modifies sur 3 résidus, A1, B1 et B29, et peuvent être séparés par chromatographie hydrophobe. La présentation par les cellules B spécifiques est inchangée pour le dérivé TNP
1-PI alors qu'elle est 50 fois amplifiée pour le dérivé TNP
2-PI en A1 et B29 (TNP
2(A1,B29)-PI); par contre, les conjugués (TNP
2(A1,B1)-PI) et (TNP
3(A1,B1,B29)-PI) sont présentés de façon comparable sur cellules transfectées ou non. Ainsi seul le conjugué le plus hydrophobe (TNP
2(A1,B29)-PI) subit une présentation plus efficace par des cellules B spécifiques. L'efficacité de présentation est directement proportionnelle au taux de métabolisation. Nos résultats démontrent que la valence et l'hydrophobicité d'un antigène déterminent son interaction avec des IgM membranaires et l'efficacité de présentation par des lymphocytes B.</description><subject>Animals</subject><subject>Antigen-Presenting Cells - immunology</subject><subject>Antigens</subject><subject>B lymphocyte, Valency, Hydrophobicity, Antigen binding, sIgM</subject><subject>B-cell processing and presentation, T cell, Derivatization, Pork insulin, TNP conjugation</subject><subject>B-Lymphocytes - immunology</subject><subject>Genes, Immunoglobulin</subject><subject>Haptens</subject><subject>Insulin - immunology</subject><subject>Lymphocyte B, Valence, Hydrophobicité, Liaison antigénique, sIgM</subject><subject>Métabolisation et présentation des cellules B, Lymphocyte T, Dérivation, Insuline de porc, TNP</subject><subject>Ovalbumin - immunology</subject><subject>Transfection</subject><subject>Trinitrobenzenes - immunology</subject><issn>0923-2494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEYhbNQaq2-gUJWoovRJHPfCFq8FApuuuguZDJ_2sg0MyZpYV7A5zbTVpdCICTnnP_yIXRFyT0lNHsgJYsjlpTJbVHelYQyEi1P0Pjv-wydO_dJCM1pzkZoxLI8ZawYo--ZUc0WjATcKuzXgHeiCc8eC1PjdV_btlu3lZba94NDmHC8XoHBrcHaOwxKBXUfCXpnWwnOabPaF-gsODBeeB3cVf-bjVwHUoccfsYSmsZdoFMlGgeXx3uCFq8vi-l7NP94m02f5pGMSeYjKrI4S0VFRBJneSYVKQiTROVUCqHSNCwOjMY1MMkqVYiKJlkOaVUUqYqliCfo5lA2jPm1Bef5RrthAGGg3Tqel4wmeZkEY3IwSts6Z0HxzuqNsD2nhA_E-YCWD2h5UfI9cb4Msetj_W21gfovdMQd9MeDDmHHnQbL3R4d1NqC9Lxu9f8NfgATxJSg</recordid><startdate>19890101</startdate><enddate>19890101</enddate><creator>Bernard, N.F</creator><creator>Naquet, P</creator><creator>Watanabe, M</creator><creator>Hozumi, N</creator><creator>Delovitch, T.L</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890101</creationdate><title>Influence of the valency and hydrophobicity of an antigen on its efficiency of processing and presentation by antigen-specific B cells</title><author>Bernard, N.F ; Naquet, P ; Watanabe, M ; Hozumi, N ; Delovitch, T.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-1a6365ab0a43676cf0802c0f71caaf55012e213de2c2bf8ab1467e5b885f3ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Antigen-Presenting Cells - immunology</topic><topic>Antigens</topic><topic>B lymphocyte, Valency, Hydrophobicity, Antigen binding, sIgM</topic><topic>B-cell processing and presentation, T cell, Derivatization, Pork insulin, TNP conjugation</topic><topic>B-Lymphocytes - immunology</topic><topic>Genes, Immunoglobulin</topic><topic>Haptens</topic><topic>Insulin - immunology</topic><topic>Lymphocyte B, Valence, Hydrophobicité, Liaison antigénique, sIgM</topic><topic>Métabolisation et présentation des cellules B, Lymphocyte T, Dérivation, Insuline de porc, TNP</topic><topic>Ovalbumin - immunology</topic><topic>Transfection</topic><topic>Trinitrobenzenes - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>Bernard, N.F</creatorcontrib><creatorcontrib>Naquet, P</creatorcontrib><creatorcontrib>Watanabe, M</creatorcontrib><creatorcontrib>Hozumi, N</creatorcontrib><creatorcontrib>Delovitch, T.L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Research in immunology (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bernard, N.F</au><au>Naquet, P</au><au>Watanabe, M</au><au>Hozumi, N</au><au>Delovitch, T.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of the valency and hydrophobicity of an antigen on its efficiency of processing and presentation by antigen-specific B cells</atitle><jtitle>Research in immunology (Paris)</jtitle><addtitle>Res Immunol</addtitle><date>1989-01-01</date><risdate>1989</risdate><volume>140</volume><issue>5</issue><spage>563</spage><epage>579</epage><pages>563-579</pages><issn>0923-2494</issn><abstract>We examined the influence of antigen structure on the efficiency of antigen-specific B-cell processing and presentation. The ability of untransfected and anti-TNP surface IgM (sIgM) bearing transfected TA3 B-hybridoma cells to process and present various conjugates of 2,4,6-trinitrophenyl-pork insulin (TNP-PI) to PI/I-A
d-specific T cells was investigated. Similar antigen concentrations were required for presentation of underivatized antigen by untransfected and transfected cells (antigen-non-specific presentation). Transfected cells present TNP-PI at about a 50-fold lower concentration and TNP-ovalbumin (TNP-OVA) at a 600-fold lower concentration than do untransfected cells (antigen-specific presentation). PI was derivatized at 3 possible residues, A1, B1 and B29, and the different TNP-PI conjugates obtained were separated by hydrophobic interaction high-pressure liquid chromatography. All TNP
1-PI conjugates were presented equally by transfected and untransfected cells. Transfected TA3 cells presented a TNP
2-PI conjugate derivatized at A1 and B29, TNP
2(A1, B29)-PI, at about a 50-fold lower concentration than was required by untransfected cells. Another TNP
2-PI conjugate, derivatized at residues A1 and B1, TNP
2(A1, B1)-PI, and a TNP
3(A1,B1,B29)-PI conjugate were presented at about the same concentration by transfected
vs untransfected B cells. Of the various TNP conjugates tested, only TNP
2(A1,B29)-PI, which is more hydrophobic than any of the other TNP-PI conjugates, was processed more efficiently by transfected anti-TNP-specific TA3 cells
vs untransfected cells. The efficiency of presentation of these TNP-PI conjugates was directly proportional to their rate of processing. Our data demonstrate that the valency and relative hydrophobicity of an antigen control its binding to sIgM and the efficiency of processing and presentation by B cells.
Nous avons évalué l'influence de la structure d'un antigène sur sa capacité d'être métabolisé et présenté par des lymphocytes B. Nous avons comparé la présentation de l'insuline de porc couplée au TNP (TNP-PI) à des hybridomes T spécifiques de PI dans le contexte I-A
d par des cellules TA3 parentales ou transfectées avec le gène codant pour une IgM membranaire spécifique du TNP. Alors que les concentrations antigéniques requises pour activer les cellules T étaient comparables en l'absence de TNP (présentation non spécifique de l'antigène), il devenait possible de les diminuer de l'ordr de 50 fois pour TNP-PI et 600 fois pour TNP-OVA en utilisant les cellules les B transfectées (présentation spécique de l'antigène). Les conjugués TNP-PI sont potentiellement modifies sur 3 résidus, A1, B1 et B29, et peuvent être séparés par chromatographie hydrophobe. La présentation par les cellules B spécifiques est inchangée pour le dérivé TNP
1-PI alors qu'elle est 50 fois amplifiée pour le dérivé TNP
2-PI en A1 et B29 (TNP
2(A1,B29)-PI); par contre, les conjugués (TNP
2(A1,B1)-PI) et (TNP
3(A1,B1,B29)-PI) sont présentés de façon comparable sur cellules transfectées ou non. Ainsi seul le conjugué le plus hydrophobe (TNP
2(A1,B29)-PI) subit une présentation plus efficace par des cellules B spécifiques. L'efficacité de présentation est directement proportionnelle au taux de métabolisation. Nos résultats démontrent que la valence et l'hydrophobicité d'un antigène déterminent son interaction avec des IgM membranaires et l'efficacité de présentation par des lymphocytes B.</abstract><cop>France</cop><pub>Elsevier B.V</pub><pmid>2675228</pmid><doi>10.1016/0923-2494(89)90120-X</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Research in immunology (Paris), 1989-01, Vol.140 (5), p.563-579 |
| issn | 0923-2494 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigen-Presenting Cells - immunology Antigens B lymphocyte, Valency, Hydrophobicity, Antigen binding, sIgM B-cell processing and presentation, T cell, Derivatization, Pork insulin, TNP conjugation B-Lymphocytes - immunology Genes, Immunoglobulin Haptens Insulin - immunology Lymphocyte B, Valence, Hydrophobicité, Liaison antigénique, sIgM Métabolisation et présentation des cellules B, Lymphocyte T, Dérivation, Insuline de porc, TNP Ovalbumin - immunology Transfection Trinitrobenzenes - immunology |
| title | Influence of the valency and hydrophobicity of an antigen on its efficiency of processing and presentation by antigen-specific B cells |
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