Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody
The product of Pseudomonas aeruginosa regA gene acts as a positive regulator of exotoxin A expression. The protein, RegA, was overproduced in E. coli transformed with an expression vector containing the regA gene. The overproduced RegA accumulated in E. coli in the form of inclusion bodies. The latt...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1989-09, Vol.163 (3), p.1312-1318 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Zimniak, Ludwika Dayn, Andrey Iglewski, Barbara H. |
| description | The product of
Pseudomonas aeruginosa regA gene acts as a positive regulator of exotoxin A expression. The protein, RegA, was overproduced in
E. coli transformed with an expression vector containing the regA gene. The overproduced RegA accumulated in
E. coli in the form of inclusion bodies. The latter were isolated and served as a source of antigen for raising polyclonal antibodies. The antibodies reacted specifically with a
P. aerugtnosa protein whose molecular weight corresponded to that predicted for RegA from its known DNA sequence, and whose response to modulating factors matched that expected for RegA. The immunodetectable RegA was localized in the membrane fraction of
P. aeruginosa strain PA103. |
| doi_str_mv | 10.1016/0006-291X(89)91121-2 |
| format | Article |
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Pseudomonas aeruginosa regA gene acts as a positive regulator of exotoxin A expression. The protein, RegA, was overproduced in
E. coli transformed with an expression vector containing the regA gene. The overproduced RegA accumulated in
E. coli in the form of inclusion bodies. The latter were isolated and served as a source of antigen for raising polyclonal antibodies. The antibodies reacted specifically with a
P. aerugtnosa protein whose molecular weight corresponded to that predicted for RegA from its known DNA sequence, and whose response to modulating factors matched that expected for RegA. The immunodetectable RegA was localized in the membrane fraction of
P. aeruginosa strain PA103.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(89)91121-2</identifier><identifier>PMID: 2506862</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ADP Ribose Transferases ; Antigen-Antibody Complex - analysis ; Bacterial Toxins ; Escherichia coli - genetics ; Exotoxins - genetics ; Genes ; Genes, Bacterial ; Genes, Regulator ; Genetic Vectors ; Immune Sera ; Molecular Weight ; Oligonucleotide Probes ; Plasmids ; Pseudomonas aeruginosa - genetics ; Pseudomonas aeruginosa Exotoxin A ; RegA protein ; Virulence Factors</subject><ispartof>Biochemical and biophysical research communications, 1989-09, Vol.163 (3), p.1312-1318</ispartof><rights>1989</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-e7d3218e9c333a8313cfb714afe5926c17e815e22871b0c1faf53689c928dbc83</citedby><cites>FETCH-LOGICAL-c434t-e7d3218e9c333a8313cfb714afe5926c17e815e22871b0c1faf53689c928dbc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X89911212$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2506862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zimniak, Ludwika</creatorcontrib><creatorcontrib>Dayn, Andrey</creatorcontrib><creatorcontrib>Iglewski, Barbara H.</creatorcontrib><title>Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The product of
Pseudomonas aeruginosa regA gene acts as a positive regulator of exotoxin A expression. The protein, RegA, was overproduced in
E. coli transformed with an expression vector containing the regA gene. The overproduced RegA accumulated in
E. coli in the form of inclusion bodies. The latter were isolated and served as a source of antigen for raising polyclonal antibodies. The antibodies reacted specifically with a
P. aerugtnosa protein whose molecular weight corresponded to that predicted for RegA from its known DNA sequence, and whose response to modulating factors matched that expected for RegA. The immunodetectable RegA was localized in the membrane fraction of
P. aeruginosa strain PA103.</description><subject>ADP Ribose Transferases</subject><subject>Antigen-Antibody Complex - analysis</subject><subject>Bacterial Toxins</subject><subject>Escherichia coli - genetics</subject><subject>Exotoxins - genetics</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Genetic Vectors</subject><subject>Immune Sera</subject><subject>Molecular Weight</subject><subject>Oligonucleotide Probes</subject><subject>Plasmids</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas aeruginosa Exotoxin A</subject><subject>RegA protein</subject><subject>Virulence Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQx4MotVa_gcKeRA-rmWQfyUUoxUdBUHyAt5DNTkqku6nJruC3d2uLRz3NwP8xw4-QY6AXQKG4pJQWKZPwdibkuQRgkLIdMgYqacqAZrtk_GvZJwcxvlMKkBVyREYsp4Uo2Jg8z2tsO2ed0Z3zbeJt8oSLabIKvkPXJjb4JnmM2Ne-8a2OicbQL1zro0766NpFood4-pNZb5Wvvw7JntXLiEfbOSGvN9cvs7v0_uF2PpvepybjWZdiWXMGAqXhnGvBgRtblZBpi7lkhYESBeTImCihogastjkvhDSSiboygk_I6aZ3ePajx9ipxkWDy6Vu0fdRlZJBlrPiXyPkPAch6GDMNkYTfIwBrVoF1-jwpYCqNXS1JqrWRJWQ6ge6YkPsZNvfVw3Wv6Et5UG_2ug40Ph0GFQ0DluDtQtoOlV79_eBb_6kkMU</recordid><startdate>19890929</startdate><enddate>19890929</enddate><creator>Zimniak, Ludwika</creator><creator>Dayn, Andrey</creator><creator>Iglewski, Barbara H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890929</creationdate><title>Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody</title><author>Zimniak, Ludwika ; Dayn, Andrey ; Iglewski, Barbara H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-e7d3218e9c333a8313cfb714afe5926c17e815e22871b0c1faf53689c928dbc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ADP Ribose Transferases</topic><topic>Antigen-Antibody Complex - analysis</topic><topic>Bacterial Toxins</topic><topic>Escherichia coli - genetics</topic><topic>Exotoxins - genetics</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Genetic Vectors</topic><topic>Immune Sera</topic><topic>Molecular Weight</topic><topic>Oligonucleotide Probes</topic><topic>Plasmids</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas aeruginosa Exotoxin A</topic><topic>RegA protein</topic><topic>Virulence Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zimniak, Ludwika</creatorcontrib><creatorcontrib>Dayn, Andrey</creatorcontrib><creatorcontrib>Iglewski, Barbara H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zimniak, Ludwika</au><au>Dayn, Andrey</au><au>Iglewski, Barbara H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1989-09-29</date><risdate>1989</risdate><volume>163</volume><issue>3</issue><spage>1312</spage><epage>1318</epage><pages>1312-1318</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The product of
Pseudomonas aeruginosa regA gene acts as a positive regulator of exotoxin A expression. The protein, RegA, was overproduced in
E. coli transformed with an expression vector containing the regA gene. The overproduced RegA accumulated in
E. coli in the form of inclusion bodies. The latter were isolated and served as a source of antigen for raising polyclonal antibodies. The antibodies reacted specifically with a
P. aerugtnosa protein whose molecular weight corresponded to that predicted for RegA from its known DNA sequence, and whose response to modulating factors matched that expected for RegA. The immunodetectable RegA was localized in the membrane fraction of
P. aeruginosa strain PA103.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2506862</pmid><doi>10.1016/0006-291X(89)91121-2</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1989-09, Vol.163 (3), p.1312-1318 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ADP Ribose Transferases Antigen-Antibody Complex - analysis Bacterial Toxins Escherichia coli - genetics Exotoxins - genetics Genes Genes, Bacterial Genes, Regulator Genetic Vectors Immune Sera Molecular Weight Oligonucleotide Probes Plasmids Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa Exotoxin A RegA protein Virulence Factors |
| title | Identification of RegA protein from Pseudomonas aeruginosa using anti-RegA antibody |
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