Overproduction of glycolytic enzymes in yeast

Eight different enzyymes for glycolysis and alcoholic fermentation were overproduced in a common Saccharomyces cerevisiae strain by placing their genes on multicopy vectors. The specific enzyme activities were increased between 3·7‐and 13·9‐fold above the wild‐type level. The overproduction of the d...

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Veröffentlicht in:	Yeast (Chichester, England) England), 1989-07, Vol.5 (4), p.285-290
Hauptverfasser:	Schaaff, Ine, Heinisch, Jürgen, Zimmermann, Friedrich K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Dehydrogenase - biosynthesis Alcohol Dehydrogenase - genetics Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Escherichia coli - genetics ethanol ethanol production Fundamental and applied biological sciences. Psychology Genetic Vectors Genetics Glycolysis Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hexokinase - biosynthesis Hexokinase - genetics metabolic flux Microbiology Mission oriented research Mycology Phosphofructokinase-1 - biosynthesis Phosphofructokinase-1 - genetics Plasmids Pyruvate Decarboxylase - biosynthesis Pyruvate Decarboxylase - genetics Pyruvate Kinase - biosynthesis Pyruvate Kinase - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Transformation, Genetic
Online-Zugang:	Volltext
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