Degradation of phospholipids and triacylglycerol, and accumulation of fatty acids in anoxic myocardial tissue, disrupted by freeze-thawing
The degradation of lipids by endogenous hydrolytic activity has been studied in rat cardiac tissue deliberately damaged by freezing and thawing prior to storage under anoxic conditions. Aliquots of the freeze-thawed material were kept at 37 degrees C under an atmosphere of N2 up to 120 minutes. Tria...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1989-06, Vol.88 (1-2), p.83-90 |
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| container_title | Molecular and cellular biochemistry |
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| creator | van der Vusse, G J de Groot, M J Willemsen, P H van Bilsen, M Schrijvers, A H Reneman, R S |
| description | The degradation of lipids by endogenous hydrolytic activity has been studied in rat cardiac tissue deliberately damaged by freezing and thawing prior to storage under anoxic conditions. Aliquots of the freeze-thawed material were kept at 37 degrees C under an atmosphere of N2 up to 120 minutes. Triacylglycerol was hydrolyzed at a rate of 0.14 mumol fatty acids per minute per gram dry weight of tissue. Hydrolysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was associated with proportional production of lyso PC and lyso PE, respectively. This finding indicates that the activity of lysophospholipase is negligible in autolyzing cardiac tissue. The rate of hydrolysis of PC and PE was found to be 0.10 and 0.06 mumol per minute per gram dry weight of tissue. The observation that lyso PC and lyso PE mainly contained saturated and mono-unsaturated fatty acids indicates that phospholipase A2 rather than A1 is active in autolyzing cardiac tissue. The accumulation of fatty acids corresponded with the loss of triacylglycerol and phospholipids from the tissue during 120 minutes of autolysis. |
| doi_str_mv | 10.1007/BF00223428 |
| format | Article |
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Aliquots of the freeze-thawed material were kept at 37 degrees C under an atmosphere of N2 up to 120 minutes. Triacylglycerol was hydrolyzed at a rate of 0.14 mumol fatty acids per minute per gram dry weight of tissue. Hydrolysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was associated with proportional production of lyso PC and lyso PE, respectively. This finding indicates that the activity of lysophospholipase is negligible in autolyzing cardiac tissue. The rate of hydrolysis of PC and PE was found to be 0.10 and 0.06 mumol per minute per gram dry weight of tissue. The observation that lyso PC and lyso PE mainly contained saturated and mono-unsaturated fatty acids indicates that phospholipase A2 rather than A1 is active in autolyzing cardiac tissue. The accumulation of fatty acids corresponded with the loss of triacylglycerol and phospholipids from the tissue during 120 minutes of autolysis.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/BF00223428</identifier><identifier>PMID: 2789332</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; anoxia ; Autolysis ; Coronary Disease - metabolism ; Coronary Disease - pathology ; fatty acids ; Fatty Acids - metabolism ; Freezing ; HEPES ; Hydrogen-Ion Concentration ; Myocardium - metabolism ; Myocardium - pathology ; Myocardium - ultrastructure ; phospholipids ; Phospholipids - metabolism ; Rats ; Rats, Inbred Lew ; Triglycerides - metabolism</subject><ispartof>Molecular and cellular biochemistry, 1989-06, Vol.88 (1-2), p.83-90</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-8ad399ea35913a226af5f1db2211f7c1a2a2d9b086f7caa6957ca77f1aafc8313</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2789332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Vusse, G J</creatorcontrib><creatorcontrib>de Groot, M J</creatorcontrib><creatorcontrib>Willemsen, P H</creatorcontrib><creatorcontrib>van Bilsen, M</creatorcontrib><creatorcontrib>Schrijvers, A H</creatorcontrib><creatorcontrib>Reneman, R S</creatorcontrib><title>Degradation of phospholipids and triacylglycerol, and accumulation of fatty acids in anoxic myocardial tissue, disrupted by freeze-thawing</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>The degradation of lipids by endogenous hydrolytic activity has been studied in rat cardiac tissue deliberately damaged by freezing and thawing prior to storage under anoxic conditions. Aliquots of the freeze-thawed material were kept at 37 degrees C under an atmosphere of N2 up to 120 minutes. Triacylglycerol was hydrolyzed at a rate of 0.14 mumol fatty acids per minute per gram dry weight of tissue. Hydrolysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was associated with proportional production of lyso PC and lyso PE, respectively. This finding indicates that the activity of lysophospholipase is negligible in autolyzing cardiac tissue. The rate of hydrolysis of PC and PE was found to be 0.10 and 0.06 mumol per minute per gram dry weight of tissue. The observation that lyso PC and lyso PE mainly contained saturated and mono-unsaturated fatty acids indicates that phospholipase A2 rather than A1 is active in autolyzing cardiac tissue. The accumulation of fatty acids corresponded with the loss of triacylglycerol and phospholipids from the tissue during 120 minutes of autolysis.</description><subject>Animals</subject><subject>anoxia</subject><subject>Autolysis</subject><subject>Coronary Disease - metabolism</subject><subject>Coronary Disease - pathology</subject><subject>fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Freezing</subject><subject>HEPES</subject><subject>Hydrogen-Ion Concentration</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - pathology</subject><subject>Myocardium - ultrastructure</subject><subject>phospholipids</subject><subject>Phospholipids - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Triglycerides - metabolism</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1PHDEQxS2UCC5AQx_JVQrEJh779rxb8hEgElIaqFdz_jgcedeL7VXY_An5q_GFE5QpRk_z9JtXzCPkBNhXYEx-u7hmjHOx5M0eWUAtRbVsof1AFkwwVjUg5QH5lNIvxgoOsE_2uWxaIfiC_L0ym4gaswsDDZaOjyGV8W50OlEcNM3RoZr9xs_KxODP_pmo1NRP_u3MYs5zcbdHbihIeHaK9nNQGLVDT7NLaTJnVLsUpzEbTdcztdGYP6bKj_jbDZsj8tGiT-Z4p4fk4fr7_eVtdffz5sfl-V2lBIhcNahF2xoUdQsCOV-hrS3oNecAVipAjly3a9asyoa4ausiUlpAtKopEYfky2vuGMPTZFLuepeU8R4HE6bUyfK7hi_Ff0GolzWrYZt4-gqqGFKKxnZjdD3GuQPWbRvq3hsq8Odd6rTujX5Dd5WIF5ggjhM</recordid><startdate>19890627</startdate><enddate>19890627</enddate><creator>van der Vusse, G J</creator><creator>de Groot, M J</creator><creator>Willemsen, P H</creator><creator>van Bilsen, M</creator><creator>Schrijvers, A H</creator><creator>Reneman, R S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890627</creationdate><title>Degradation of phospholipids and triacylglycerol, and accumulation of fatty acids in anoxic myocardial tissue, disrupted by freeze-thawing</title><author>van der Vusse, G J ; de Groot, M J ; Willemsen, P H ; van Bilsen, M ; Schrijvers, A H ; Reneman, R S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-8ad399ea35913a226af5f1db2211f7c1a2a2d9b086f7caa6957ca77f1aafc8313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>anoxia</topic><topic>Autolysis</topic><topic>Coronary Disease - metabolism</topic><topic>Coronary Disease - pathology</topic><topic>fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Freezing</topic><topic>HEPES</topic><topic>Hydrogen-Ion Concentration</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - pathology</topic><topic>Myocardium - ultrastructure</topic><topic>phospholipids</topic><topic>Phospholipids - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Vusse, G J</creatorcontrib><creatorcontrib>de Groot, M J</creatorcontrib><creatorcontrib>Willemsen, P H</creatorcontrib><creatorcontrib>van Bilsen, M</creatorcontrib><creatorcontrib>Schrijvers, A H</creatorcontrib><creatorcontrib>Reneman, R S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Vusse, G J</au><au>de Groot, M J</au><au>Willemsen, P H</au><au>van Bilsen, M</au><au>Schrijvers, A H</au><au>Reneman, R S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradation of phospholipids and triacylglycerol, and accumulation of fatty acids in anoxic myocardial tissue, disrupted by freeze-thawing</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1989-06-27</date><risdate>1989</risdate><volume>88</volume><issue>1-2</issue><spage>83</spage><epage>90</epage><pages>83-90</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>The degradation of lipids by endogenous hydrolytic activity has been studied in rat cardiac tissue deliberately damaged by freezing and thawing prior to storage under anoxic conditions. Aliquots of the freeze-thawed material were kept at 37 degrees C under an atmosphere of N2 up to 120 minutes. Triacylglycerol was hydrolyzed at a rate of 0.14 mumol fatty acids per minute per gram dry weight of tissue. Hydrolysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was associated with proportional production of lyso PC and lyso PE, respectively. This finding indicates that the activity of lysophospholipase is negligible in autolyzing cardiac tissue. The rate of hydrolysis of PC and PE was found to be 0.10 and 0.06 mumol per minute per gram dry weight of tissue. The observation that lyso PC and lyso PE mainly contained saturated and mono-unsaturated fatty acids indicates that phospholipase A2 rather than A1 is active in autolyzing cardiac tissue. The accumulation of fatty acids corresponded with the loss of triacylglycerol and phospholipids from the tissue during 120 minutes of autolysis.</abstract><cop>Netherlands</cop><pmid>2789332</pmid><doi>10.1007/BF00223428</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 1989-06, Vol.88 (1-2), p.83-90 |
| issn | 0300-8177 1573-4919 |
| language | eng |
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| subjects | Animals anoxia Autolysis Coronary Disease - metabolism Coronary Disease - pathology fatty acids Fatty Acids - metabolism Freezing HEPES Hydrogen-Ion Concentration Myocardium - metabolism Myocardium - pathology Myocardium - ultrastructure phospholipids Phospholipids - metabolism Rats Rats, Inbred Lew Triglycerides - metabolism |
| title | Degradation of phospholipids and triacylglycerol, and accumulation of fatty acids in anoxic myocardial tissue, disrupted by freeze-thawing |
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