DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene
The immediate early (IE) proteins of herpesviruses are important regulatory factors which control the expression of genes the transcriptional level. We report the DNA sequence of the immediate arly gene of the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This sequence is shown to be extremely...
Gespeichert in:
| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1989-09, Vol.172 (1), p.223-236 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 236 |
|---|---|
| container_issue | 1 |
| container_start_page | 223 |
| container_title | Virology (New York, N.Y.) |
| container_volume | 172 |
| creator | Grundy, Frank J. Baumann, Raymond P. O'Callaghani, Dennis J. |
| description | The immediate early (IE) proteins of herpesviruses are important regulatory factors which control the expression of genes the transcriptional level. We report the DNA sequence of the immediate arly gene of the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This sequence is shown to be extremely rich in guanine and cytosine, resulting in a highly biased codon usage. The IE gene region possesses 38 open reading frames (ORFs) greater than 300 by in length, 11 of which have coding regions of at least 100 amino acids (aa) following potential translation initiator codons. The largest ORF consists of 1487 codons (4461 bp) starting with the first ATG and would encode a protein of MW 155,000. TATA and CCAAT sequences as well as several potential cis-acting elements lie upstream to the major ORF. The deduced amino acid sequence for the 155,000 protein has a high degree of homology to the herpes simplex virus type 1 (HSV-1) ICP4 protein and its varicella-zoster virus (VZV) homolog. The regions of the EHV-1 IE protein that are homologous with these proteins correspond to the previously determined pattern of homology between the HSV and VZVIE polypeptides. However, there are are a number of differences within these broadly defined regions. It is therefore expected that this comparative study will facilitate the identification of functionally important residues within the amino acid sequence of IE proteins. |
| doi_str_mv | 10.1016/0042-6822(89)90124-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79195025</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0042682289901244</els_id><sourcerecordid>79195025</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-35e3af1374dfbeee24aaf9e56f9b2d33319e90bca1e8ab94436792ab252184433</originalsourceid><addsrcrecordid>eNqFkUtr3DAURkVoSadp_kBoQYtS0oVTvWxZm0JI0weEdtFklYW4lq8SFb8qeQbm30fODLNsV-LjO_ciHRHylrMLznj1iTEliqoW4rw2Hw3jQhXqiKw4M1XBpOIvyOqAvCKvU_rDctaaHZNjUSqjOV-R-y8_L2nCv2scHFIYWurGfoIIc9gsGbptwkRHT-dHpJkLA9JHjBOmTYjrROfthJTT0PfYBpgzA7Hb0gcc8A156aFLeLo_T8jd1-vbq-_Fza9vP64ubwqnZDUXskQJnkutWt8golAA3mBZedOIVkrJDRrWOOBYQ2NUHtJGQCNKweuc5An5sNs7xTE_JM22D8lh18GA4zpZbbgpmSj_C_JS1oZpkUG1A10cU4ro7RRDD3FrObOLfLuYtYtZWxv7LN-qPPZuv3_dZB2Hob3t3L_f95AcdD7C4EI6YFqWmokqY2c7zMNo4SFm5O63YcxIU-fy867EbHQTMNrkwvJ5bYjoZtuO4d-XfAK0AqfX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15389072</pqid></control><display><type>article</type><title>DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Grundy, Frank J. ; Baumann, Raymond P. ; O'Callaghani, Dennis J.</creator><creatorcontrib>Grundy, Frank J. ; Baumann, Raymond P. ; O'Callaghani, Dennis J.</creatorcontrib><description>The immediate early (IE) proteins of herpesviruses are important regulatory factors which control the expression of genes the transcriptional level. We report the DNA sequence of the immediate arly gene of the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This sequence is shown to be extremely rich in guanine and cytosine, resulting in a highly biased codon usage. The IE gene region possesses 38 open reading frames (ORFs) greater than 300 by in length, 11 of which have coding regions of at least 100 amino acids (aa) following potential translation initiator codons. The largest ORF consists of 1487 codons (4461 bp) starting with the first ATG and would encode a protein of MW 155,000. TATA and CCAAT sequences as well as several potential cis-acting elements lie upstream to the major ORF. The deduced amino acid sequence for the 155,000 protein has a high degree of homology to the herpes simplex virus type 1 (HSV-1) ICP4 protein and its varicella-zoster virus (VZV) homolog. The regions of the EHV-1 IE protein that are homologous with these proteins correspond to the previously determined pattern of homology between the HSV and VZVIE polypeptides. However, there are are a number of differences within these broadly defined regions. It is therefore expected that this comparative study will facilitate the identification of functionally important residues within the amino acid sequence of IE proteins.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(89)90124-4</identifier><identifier>PMID: 2549711</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; CABALLOS ; CHEVAL ; Codon ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Viral ; Genetics ; GENOMAS ; GENOME ; GENOMES ; Herpesviridae - genetics ; Herpesvirus 1, Equid - genetics ; HERPETOVIRIDAE ; HORSES ; Immediate-Early Proteins ; Microbiology ; Molecular Sequence Data ; PEPTIDE ; PEPTIDES ; PEPTIDOS ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; Poly A - genetics ; POLYPEPTIDES ; Regulatory Sequences, Nucleic Acid ; Viral Proteins - genetics ; Virology</subject><ispartof>Virology (New York, N.Y.), 1989-09, Vol.172 (1), p.223-236</ispartof><rights>1989</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-35e3af1374dfbeee24aaf9e56f9b2d33319e90bca1e8ab94436792ab252184433</citedby><cites>FETCH-LOGICAL-c436t-35e3af1374dfbeee24aaf9e56f9b2d33319e90bca1e8ab94436792ab252184433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682289901244$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7357026$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2549711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grundy, Frank J.</creatorcontrib><creatorcontrib>Baumann, Raymond P.</creatorcontrib><creatorcontrib>O'Callaghani, Dennis J.</creatorcontrib><title>DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The immediate early (IE) proteins of herpesviruses are important regulatory factors which control the expression of genes the transcriptional level. We report the DNA sequence of the immediate arly gene of the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This sequence is shown to be extremely rich in guanine and cytosine, resulting in a highly biased codon usage. The IE gene region possesses 38 open reading frames (ORFs) greater than 300 by in length, 11 of which have coding regions of at least 100 amino acids (aa) following potential translation initiator codons. The largest ORF consists of 1487 codons (4461 bp) starting with the first ATG and would encode a protein of MW 155,000. TATA and CCAAT sequences as well as several potential cis-acting elements lie upstream to the major ORF. The deduced amino acid sequence for the 155,000 protein has a high degree of homology to the herpes simplex virus type 1 (HSV-1) ICP4 protein and its varicella-zoster virus (VZV) homolog. The regions of the EHV-1 IE protein that are homologous with these proteins correspond to the previously determined pattern of homology between the HSV and VZVIE polypeptides. However, there are are a number of differences within these broadly defined regions. It is therefore expected that this comparative study will facilitate the identification of functionally important residues within the amino acid sequence of IE proteins.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CABALLOS</subject><subject>CHEVAL</subject><subject>Codon</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>GENOMAS</subject><subject>GENOME</subject><subject>GENOMES</subject><subject>Herpesviridae - genetics</subject><subject>Herpesvirus 1, Equid - genetics</subject><subject>HERPETOVIRIDAE</subject><subject>HORSES</subject><subject>Immediate-Early Proteins</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>PEPTIDE</subject><subject>PEPTIDES</subject><subject>PEPTIDOS</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>Poly A - genetics</subject><subject>POLYPEPTIDES</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3DAURkVoSadp_kBoQYtS0oVTvWxZm0JI0weEdtFklYW4lq8SFb8qeQbm30fODLNsV-LjO_ciHRHylrMLznj1iTEliqoW4rw2Hw3jQhXqiKw4M1XBpOIvyOqAvCKvU_rDctaaHZNjUSqjOV-R-y8_L2nCv2scHFIYWurGfoIIc9gsGbptwkRHT-dHpJkLA9JHjBOmTYjrROfthJTT0PfYBpgzA7Hb0gcc8A156aFLeLo_T8jd1-vbq-_Fza9vP64ubwqnZDUXskQJnkutWt8golAA3mBZedOIVkrJDRrWOOBYQ2NUHtJGQCNKweuc5An5sNs7xTE_JM22D8lh18GA4zpZbbgpmSj_C_JS1oZpkUG1A10cU4ro7RRDD3FrObOLfLuYtYtZWxv7LN-qPPZuv3_dZB2Hob3t3L_f95AcdD7C4EI6YFqWmokqY2c7zMNo4SFm5O63YcxIU-fy867EbHQTMNrkwvJ5bYjoZtuO4d-XfAK0AqfX</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Grundy, Frank J.</creator><creator>Baumann, Raymond P.</creator><creator>O'Callaghani, Dennis J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890901</creationdate><title>DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene</title><author>Grundy, Frank J. ; Baumann, Raymond P. ; O'Callaghani, Dennis J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-35e3af1374dfbeee24aaf9e56f9b2d33319e90bca1e8ab94436792ab252184433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CABALLOS</topic><topic>CHEVAL</topic><topic>Codon</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Viral</topic><topic>Genetics</topic><topic>GENOMAS</topic><topic>GENOME</topic><topic>GENOMES</topic><topic>Herpesviridae - genetics</topic><topic>Herpesvirus 1, Equid - genetics</topic><topic>HERPETOVIRIDAE</topic><topic>HORSES</topic><topic>Immediate-Early Proteins</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>PEPTIDE</topic><topic>PEPTIDES</topic><topic>PEPTIDOS</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>Poly A - genetics</topic><topic>POLYPEPTIDES</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grundy, Frank J.</creatorcontrib><creatorcontrib>Baumann, Raymond P.</creatorcontrib><creatorcontrib>O'Callaghani, Dennis J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grundy, Frank J.</au><au>Baumann, Raymond P.</au><au>O'Callaghani, Dennis J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>172</volume><issue>1</issue><spage>223</spage><epage>236</epage><pages>223-236</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The immediate early (IE) proteins of herpesviruses are important regulatory factors which control the expression of genes the transcriptional level. We report the DNA sequence of the immediate arly gene of the alphaherpesvirus equine herpesvirus type 1 (EHV-1). This sequence is shown to be extremely rich in guanine and cytosine, resulting in a highly biased codon usage. The IE gene region possesses 38 open reading frames (ORFs) greater than 300 by in length, 11 of which have coding regions of at least 100 amino acids (aa) following potential translation initiator codons. The largest ORF consists of 1487 codons (4461 bp) starting with the first ATG and would encode a protein of MW 155,000. TATA and CCAAT sequences as well as several potential cis-acting elements lie upstream to the major ORF. The deduced amino acid sequence for the 155,000 protein has a high degree of homology to the herpes simplex virus type 1 (HSV-1) ICP4 protein and its varicella-zoster virus (VZV) homolog. The regions of the EHV-1 IE protein that are homologous with these proteins correspond to the previously determined pattern of homology between the HSV and VZVIE polypeptides. However, there are are a number of differences within these broadly defined regions. It is therefore expected that this comparative study will facilitate the identification of functionally important residues within the amino acid sequence of IE proteins.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2549711</pmid><doi>10.1016/0042-6822(89)90124-4</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1989-09, Vol.172 (1), p.223-236 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79195025 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences CABALLOS CHEVAL Codon DNA, Viral - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genes, Viral Genetics GENOMAS GENOME GENOMES Herpesviridae - genetics Herpesvirus 1, Equid - genetics HERPETOVIRIDAE HORSES Immediate-Early Proteins Microbiology Molecular Sequence Data PEPTIDE PEPTIDES PEPTIDOS PLASMIDE PLASMIDIOS PLASMIDS Poly A - genetics POLYPEPTIDES Regulatory Sequences, Nucleic Acid Viral Proteins - genetics Virology |
| title | DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T06%3A49%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20sequence%20and%20comparative%20analyses%20of%20the%20equine%20herpesvirus%20type%201%20immediate%20early%20gene&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Grundy,%20Frank%20J.&rft.date=1989-09-01&rft.volume=172&rft.issue=1&rft.spage=223&rft.epage=236&rft.pages=223-236&rft.issn=0042-6822&rft.eissn=1096-0341&rft.coden=VIRLAX&rft_id=info:doi/10.1016/0042-6822(89)90124-4&rft_dat=%3Cproquest_cross%3E79195025%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15389072&rft_id=info:pmid/2549711&rft_els_id=0042682289901244&rfr_iscdi=true |