Determination of HDL2 cholesterol by precipitation with dextran sulfate and magnesium chloride: Establishing optimal conditions for rat plasma

Optimal conditions for analyzing HDL2 cholesterol in small amounts of rat plasma have been studied using different concentrations of dextran sulfate and MgCl2 to precipitate lipoproteins containing apolipoprotein B and/or apo E. When the MgCl2 level was 91 mM, the supernate cholesterol was rather co...

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Veröffentlicht in:	Lipids 1989-06, Vol.24 (6), p.532-534
Hauptverfasser:	Sjoblom, Linnea, Eklund, Anders
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cholesterol, HDL - blood Cholesterol, HDL - isolation & purification Dextran Sulfate Dextrans Electrophoresis, Agar Gel Fractional Precipitation Fundamental and applied biological sciences. Psychology Hypercholesterolemia - blood Lipoproteins - blood Magnesium Magnesium Chloride Male Other biological molecules Rats Terpenes, steroids. Hormones
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