Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study

A technique is described for the detection of HSV-DNA in very small volumes (5–10 μl) of cerebrospinal fluid (CSF). The method was evaluated in CSF samples of 4–6-week-old mice inoculated with HSV-1 via the corneal route. The sensitivity of the PCR assay was compared with results of spin-amplified v...

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Veröffentlicht in:	Journal of virological methods 1989-08, Vol.25 (2), p.189-197
Hauptverfasser:	Boerman, R.H., Arnoldus, E.P.J., Raap, A.K., Bloem, B.R., Verhey, M., van Gemert, G., Peters, A.C.B., van der Ploeg, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cerebrospinal fluid DNA, Viral - cerebrospinal fluid DNA-Directed DNA Polymerase - metabolism encephalitis Encephalitis - cerebrospinal fluid Encephalitis - diagnosis Experimental HSV encephalitis Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Amplification Herpes Simplex - cerebrospinal fluid Herpes Simplex - diagnosis Herpes simplex virus type 1 Humans Immunoblotting Male Mice Microbiology Nucleic acid hybridization Polymerase chain reaction Simplexvirus - genetics Simplexvirus - isolation & purification Techniques used in virology Viral culture Virology
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