Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study
A technique is described for the detection of HSV-DNA in very small volumes (5–10 μl) of cerebrospinal fluid (CSF). The method was evaluated in CSF samples of 4–6-week-old mice inoculated with HSV-1 via the corneal route. The sensitivity of the PCR assay was compared with results of spin-amplified v...
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| Veröffentlicht in: | Journal of virological methods 1989-08, Vol.25 (2), p.189-197 |
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| container_title | Journal of virological methods |
| container_volume | 25 |
| creator | Boerman, R.H. Arnoldus, E.P.J. Raap, A.K. Bloem, B.R. Verhey, M. van Gemert, G. Peters, A.C.B. van der Ploeg, M. |
| description | A technique is described for the detection of HSV-DNA in very small volumes (5–10 μl) of cerebrospinal fluid (CSF). The method was evaluated in CSF samples of 4–6-week-old mice inoculated with HSV-1 via the corneal route. The sensitivity of the PCR assay was compared with results of spin-amplified viral culture with immunofluorescent visualization (SAC/IF), routine viral culture (RVC) and radioactive dot-blot hybridization (DBA) in CSF samples obtained from other mice. The results show the PCR to be superior over the other techniques: infectious virus or viral DNA in CSF was demonstrated by PCR, SAC/IF, RVC and DBA in 68, 55, 20 and 2.5%, respectively. These results show the feasibility of the PCR for a rapid, non-invasive diagnosis of human HSV-encephalitis. |
| doi_str_mv | 10.1016/0166-0934(89)90032-3 |
| format | Article |
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The method was evaluated in CSF samples of 4–6-week-old mice inoculated with HSV-1 via the corneal route. The sensitivity of the PCR assay was compared with results of spin-amplified viral culture with immunofluorescent visualization (SAC/IF), routine viral culture (RVC) and radioactive dot-blot hybridization (DBA) in CSF samples obtained from other mice. The results show the PCR to be superior over the other techniques: infectious virus or viral DNA in CSF was demonstrated by PCR, SAC/IF, RVC and DBA in 68, 55, 20 and 2.5%, respectively. These results show the feasibility of the PCR for a rapid, non-invasive diagnosis of human HSV-encephalitis.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(89)90032-3</identifier><identifier>PMID: 2550503</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cerebrospinal fluid ; DNA, Viral - cerebrospinal fluid ; DNA-Directed DNA Polymerase - metabolism ; encephalitis ; Encephalitis - cerebrospinal fluid ; Encephalitis - diagnosis ; Experimental HSV encephalitis ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; Herpes Simplex - cerebrospinal fluid ; Herpes Simplex - diagnosis ; Herpes simplex virus type 1 ; Humans ; Immunoblotting ; Male ; Mice ; Microbiology ; Nucleic acid hybridization ; Polymerase chain reaction ; Simplexvirus - genetics ; Simplexvirus - isolation & purification ; Techniques used in virology ; Viral culture ; Virology</subject><ispartof>Journal of virological methods, 1989-08, Vol.25 (2), p.189-197</ispartof><rights>1989</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-c26e119f28d0a5618745ac85958c85f351660bef479cbcb806230d0f38a8ea0d3</citedby><cites>FETCH-LOGICAL-c417t-c26e119f28d0a5618745ac85958c85f351660bef479cbcb806230d0f38a8ea0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0166093489900323$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7353414$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2550503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boerman, R.H.</creatorcontrib><creatorcontrib>Arnoldus, E.P.J.</creatorcontrib><creatorcontrib>Raap, A.K.</creatorcontrib><creatorcontrib>Bloem, B.R.</creatorcontrib><creatorcontrib>Verhey, M.</creatorcontrib><creatorcontrib>van Gemert, G.</creatorcontrib><creatorcontrib>Peters, A.C.B.</creatorcontrib><creatorcontrib>van der Ploeg, M.</creatorcontrib><title>Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A technique is described for the detection of HSV-DNA in very small volumes (5–10 μl) of cerebrospinal fluid (CSF). The method was evaluated in CSF samples of 4–6-week-old mice inoculated with HSV-1 via the corneal route. The sensitivity of the PCR assay was compared with results of spin-amplified viral culture with immunofluorescent visualization (SAC/IF), routine viral culture (RVC) and radioactive dot-blot hybridization (DBA) in CSF samples obtained from other mice. The results show the PCR to be superior over the other techniques: infectious virus or viral DNA in CSF was demonstrated by PCR, SAC/IF, RVC and DBA in 68, 55, 20 and 2.5%, respectively. These results show the feasibility of the PCR for a rapid, non-invasive diagnosis of human HSV-encephalitis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cerebrospinal fluid</subject><subject>DNA, Viral - cerebrospinal fluid</subject><subject>DNA-Directed DNA Polymerase - metabolism</subject><subject>encephalitis</subject><subject>Encephalitis - cerebrospinal fluid</subject><subject>Encephalitis - diagnosis</subject><subject>Experimental HSV encephalitis</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Herpes Simplex - cerebrospinal fluid</subject><subject>Herpes Simplex - diagnosis</subject><subject>Herpes simplex virus type 1</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Male</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Nucleic acid hybridization</subject><subject>Polymerase chain reaction</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - isolation & purification</subject><subject>Techniques used in virology</subject><subject>Viral culture</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU2LFDEQDaKss6v_QCEHET20Jp1Od4IgyLK6woKCH9eQTqqZSLp7TNKD80f8vVY7wxz1kISqevVSrx4hTzh7xRlvX-NpK6ZF80Lpl5oxUVfiHtlw1WlMq-Y-2ZwhD8llzj8YY7IT4oJc1FIyycSG_P48x8MIyWagbmvDRBNYV8I8UTt5ug_JRuqWWJYEtIDbTuHnApmWmXrAuNDbL98ptuXRxkj3c1xGLM8DdZCgT3PehQkphrgE_wY5KfzaQQojTAXT47zgxzA52G1tDCVkmsviD4_Ig8HGDI9P7xX59v7m6_Vtdffpw8frd3eVa3hXKle3wLkeauWZlS1Kb6R1Smqp8B6ERP2sh6HptOtdr1hbC-bZIJRVYJkXV-T5kXeX5lVXMWPIDmK0E-BoptNcc8W6_wK5FErhUhHYHIEOtecEg9mhWpsOhjOz-mZWU8xqilHa_PXNCGx7euJf-hH8uelkFNafneo2OxuHZCcX8hnWCSka3iDs7REGuLR9gGSyC-t6fUholvFz-PccfwCf17Zi</recordid><startdate>19890801</startdate><enddate>19890801</enddate><creator>Boerman, R.H.</creator><creator>Arnoldus, E.P.J.</creator><creator>Raap, A.K.</creator><creator>Bloem, B.R.</creator><creator>Verhey, M.</creator><creator>van Gemert, G.</creator><creator>Peters, A.C.B.</creator><creator>van der Ploeg, M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890801</creationdate><title>Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study</title><author>Boerman, R.H. ; Arnoldus, E.P.J. ; Raap, A.K. ; Bloem, B.R. ; Verhey, M. ; van Gemert, G. ; Peters, A.C.B. ; van der Ploeg, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-c26e119f28d0a5618745ac85958c85f351660bef479cbcb806230d0f38a8ea0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cerebrospinal fluid</topic><topic>DNA, Viral - cerebrospinal fluid</topic><topic>DNA-Directed DNA Polymerase - metabolism</topic><topic>encephalitis</topic><topic>Encephalitis - cerebrospinal fluid</topic><topic>Encephalitis - diagnosis</topic><topic>Experimental HSV encephalitis</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>Herpes Simplex - cerebrospinal fluid</topic><topic>Herpes Simplex - diagnosis</topic><topic>Herpes simplex virus type 1</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Male</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Nucleic acid hybridization</topic><topic>Polymerase chain reaction</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - isolation & purification</topic><topic>Techniques used in virology</topic><topic>Viral culture</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boerman, R.H.</creatorcontrib><creatorcontrib>Arnoldus, E.P.J.</creatorcontrib><creatorcontrib>Raap, A.K.</creatorcontrib><creatorcontrib>Bloem, B.R.</creatorcontrib><creatorcontrib>Verhey, M.</creatorcontrib><creatorcontrib>van Gemert, G.</creatorcontrib><creatorcontrib>Peters, A.C.B.</creatorcontrib><creatorcontrib>van der Ploeg, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boerman, R.H.</au><au>Arnoldus, E.P.J.</au><au>Raap, A.K.</au><au>Bloem, B.R.</au><au>Verhey, M.</au><au>van Gemert, G.</au><au>Peters, A.C.B.</au><au>van der Ploeg, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1989-08-01</date><risdate>1989</risdate><volume>25</volume><issue>2</issue><spage>189</spage><epage>197</epage><pages>189-197</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>A technique is described for the detection of HSV-DNA in very small volumes (5–10 μl) of cerebrospinal fluid (CSF). The method was evaluated in CSF samples of 4–6-week-old mice inoculated with HSV-1 via the corneal route. The sensitivity of the PCR assay was compared with results of spin-amplified viral culture with immunofluorescent visualization (SAC/IF), routine viral culture (RVC) and radioactive dot-blot hybridization (DBA) in CSF samples obtained from other mice. The results show the PCR to be superior over the other techniques: infectious virus or viral DNA in CSF was demonstrated by PCR, SAC/IF, RVC and DBA in 68, 55, 20 and 2.5%, respectively. These results show the feasibility of the PCR for a rapid, non-invasive diagnosis of human HSV-encephalitis.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2550503</pmid><doi>10.1016/0166-0934(89)90032-3</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of virological methods, 1989-08, Vol.25 (2), p.189-197 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Cerebrospinal fluid DNA, Viral - cerebrospinal fluid DNA-Directed DNA Polymerase - metabolism encephalitis Encephalitis - cerebrospinal fluid Encephalitis - diagnosis Experimental HSV encephalitis Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Amplification Herpes Simplex - cerebrospinal fluid Herpes Simplex - diagnosis Herpes simplex virus type 1 Humans Immunoblotting Male Mice Microbiology Nucleic acid hybridization Polymerase chain reaction Simplexvirus - genetics Simplexvirus - isolation & purification Techniques used in virology Viral culture Virology |
| title | Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid; an experimental mouse encephalitis study |
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