The oligosaccharide chains of cobra venom factor are required for complement activation
To examine the function of the carbohydrate chains of cobra venom factor (CVF), the molecule was enzymatically deglycosylated under non-denaturing conditions with N-glycanase (peptide- N 4-( N-acetyl-β-glucosaminyl) asparagine amidase). The deglycosylation of CVF chains seems to proceed independentl...
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| Veröffentlicht in: | Molecular immunology 1989-06, Vol.26 (6), p.563-574 |
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| container_title | Molecular immunology |
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| creator | Grier, Alice H. Vogel, Carl Wilhelm |
| description | To examine the function of the carbohydrate chains of cobra venom factor (CVF), the molecule was enzymatically deglycosylated under non-denaturing conditions with
N-glycanase (peptide-
N
4-(
N-acetyl-β-glucosaminyl) asparagine amidase). The deglycosylation of CVF chains seems to proceed independently of each other, leading to partially deglycosylated intermediates. Complete deglyeosylation of CVF was found to abolish the activity of CVF. The deglycosylated molecule is unable to activate the alternative pathway of complement. Deglycosylated CVF no longer consumes the serum complement activity, it does not induce C3 activation in serum, nor does it induce complement-mediated hemolysis. These results indicate that the carbohydrate moieties of CVF are essential for its role in complement activation. |
| doi_str_mv | 10.1016/0161-5890(89)90008-4 |
| format | Article |
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N-glycanase (peptide-
N
4-(
N-acetyl-β-glucosaminyl) asparagine amidase). The deglycosylation of CVF chains seems to proceed independently of each other, leading to partially deglycosylated intermediates. Complete deglyeosylation of CVF was found to abolish the activity of CVF. The deglycosylated molecule is unable to activate the alternative pathway of complement. Deglycosylated CVF no longer consumes the serum complement activity, it does not induce C3 activation in serum, nor does it induce complement-mediated hemolysis. These results indicate that the carbohydrate moieties of CVF are essential for its role in complement activation.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/0161-5890(89)90008-4</identifier><identifier>PMID: 2770749</identifier><identifier>CODEN: MOIMD5</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Complement ; Complement Activation - drug effects ; Elapid Venoms - pharmacology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glycoside Hydrolases - pharmacology ; Glycosylation ; Guinea Pigs ; Humans ; Molecular immunology ; Oligosaccharides - pharmacology</subject><ispartof>Molecular immunology, 1989-06, Vol.26 (6), p.563-574</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-e1f5d03f010276632b27aaab2b00a12a0547e0ab65e2b06b96f1cabadb6c62e23</citedby><cites>FETCH-LOGICAL-c432t-e1f5d03f010276632b27aaab2b00a12a0547e0ab65e2b06b96f1cabadb6c62e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0161589089900084$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6958890$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2770749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grier, Alice H.</creatorcontrib><creatorcontrib>Vogel, Carl Wilhelm</creatorcontrib><title>The oligosaccharide chains of cobra venom factor are required for complement activation</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>To examine the function of the carbohydrate chains of cobra venom factor (CVF), the molecule was enzymatically deglycosylated under non-denaturing conditions with
N-glycanase (peptide-
N
4-(
N-acetyl-β-glucosaminyl) asparagine amidase). The deglycosylation of CVF chains seems to proceed independently of each other, leading to partially deglycosylated intermediates. Complete deglyeosylation of CVF was found to abolish the activity of CVF. The deglycosylated molecule is unable to activate the alternative pathway of complement. Deglycosylated CVF no longer consumes the serum complement activity, it does not induce C3 activation in serum, nor does it induce complement-mediated hemolysis. These results indicate that the carbohydrate moieties of CVF are essential for its role in complement activation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Complement</subject><subject>Complement Activation - drug effects</subject><subject>Elapid Venoms - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoside Hydrolases - pharmacology</subject><subject>Glycosylation</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>Molecular immunology</subject><subject>Oligosaccharides - pharmacology</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rHDEMhk1pSDdp_0ELPpSSHiaVvTP2-BIooR-BQC8JPRrZIzcuM-ONPbvQf19vd9ljD0IgPXoRD2NvBVwLEOpTLdF0vYGr3nw0ANA37Qu2Er2WjRGtfMlWJ-QVuyjld2UUqO6cnUutQbdmxX4-PBFPY_yVCnr_hDkOxGuPc-EpcJ9cRr6jOU08oF9S5piJZ3rexkwDD3Xg07QZaaJ54ZWIO1ximl-zs4BjoTfHfskev355uP3e3P_4dnf7-b7x7VouDYnQDbAOIEBqpdbSSY2ITjoAFBKhazUBOtVRHSlnVBAeHQ5OeSVJri_Zh0PuJqfnLZXFTrF4GkecKW2L1Ub0IHtdwfYA-pxKyRTsJscJ8x8rwO592r0su5dle2P_-bRtPXt3zN-6iYbT0VFg3b8_7rF4HEPG2cdywpTp-ppYsZsDRtXFLlK2xUeaPQ1Vo1_skOL___gLexaR8Q</recordid><startdate>19890601</startdate><enddate>19890601</enddate><creator>Grier, Alice H.</creator><creator>Vogel, Carl Wilhelm</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890601</creationdate><title>The oligosaccharide chains of cobra venom factor are required for complement activation</title><author>Grier, Alice H. ; Vogel, Carl Wilhelm</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-e1f5d03f010276632b27aaab2b00a12a0547e0ab65e2b06b96f1cabadb6c62e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Complement</topic><topic>Complement Activation - drug effects</topic><topic>Elapid Venoms - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glycoside Hydrolases - pharmacology</topic><topic>Glycosylation</topic><topic>Guinea Pigs</topic><topic>Humans</topic><topic>Molecular immunology</topic><topic>Oligosaccharides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grier, Alice H.</creatorcontrib><creatorcontrib>Vogel, Carl Wilhelm</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grier, Alice H.</au><au>Vogel, Carl Wilhelm</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The oligosaccharide chains of cobra venom factor are required for complement activation</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>1989-06-01</date><risdate>1989</risdate><volume>26</volume><issue>6</issue><spage>563</spage><epage>574</epage><pages>563-574</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><coden>MOIMD5</coden><abstract>To examine the function of the carbohydrate chains of cobra venom factor (CVF), the molecule was enzymatically deglycosylated under non-denaturing conditions with
N-glycanase (peptide-
N
4-(
N-acetyl-β-glucosaminyl) asparagine amidase). The deglycosylation of CVF chains seems to proceed independently of each other, leading to partially deglycosylated intermediates. Complete deglyeosylation of CVF was found to abolish the activity of CVF. The deglycosylated molecule is unable to activate the alternative pathway of complement. Deglycosylated CVF no longer consumes the serum complement activity, it does not induce C3 activation in serum, nor does it induce complement-mediated hemolysis. These results indicate that the carbohydrate moieties of CVF are essential for its role in complement activation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2770749</pmid><doi>10.1016/0161-5890(89)90008-4</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0161-5890 |
| ispartof | Molecular immunology, 1989-06, Vol.26 (6), p.563-574 |
| issn | 0161-5890 1872-9142 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79180287 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Complement Complement Activation - drug effects Elapid Venoms - pharmacology Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoside Hydrolases - pharmacology Glycosylation Guinea Pigs Humans Molecular immunology Oligosaccharides - pharmacology |
| title | The oligosaccharide chains of cobra venom factor are required for complement activation |
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