Electron microscopy of gold-labeled human and equine chromosomes
We present an immunochemical technique for the detection of 5-bromo-2'-deoxyuridine (BrdU) incorporated discontinuously into the chromosomal DNA. A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for el...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1989-09, Vol.37 (9), p.1443-1447 |
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| container_title | The journal of histochemistry and cytochemistry |
| container_volume | 37 |
| creator | Messier, PE Drouin, R Richer, CL |
| description | We present an immunochemical technique for the detection of 5-bromo-2'-deoxyuridine (BrdU) incorporated discontinuously into the chromosomal DNA. A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for electron microscopy (EM). Well-defined bands, symmetry of sister chromatids, concordance between homologues, and band patterns similar to those observed by light microscopy facilitate chromosome identification and karyotyping. From prophase to late metaphase, chromosomes condense and bands appear to fuse. The fusion appears to be owing to chromatin reorganization. Our results underline the value of using immunogold reagents, which are ideal probes for antigen localization on chromosomes. |
| doi_str_mv | 10.1177/37.9.2768813 |
| format | Article |
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A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for electron microscopy (EM). Well-defined bands, symmetry of sister chromatids, concordance between homologues, and band patterns similar to those observed by light microscopy facilitate chromosome identification and karyotyping. From prophase to late metaphase, chromosomes condense and bands appear to fuse. The fusion appears to be owing to chromatin reorganization. Our results underline the value of using immunogold reagents, which are ideal probes for antigen localization on chromosomes.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/37.9.2768813</identifier><identifier>PMID: 2768813</identifier><identifier>CODEN: JHCYAS</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Bromodeoxyuridine - metabolism ; Chromosome Banding - methods ; Chromosomes - metabolism ; Chromosomes - ultrastructure ; Chromosomes, Human - metabolism ; Chromosomes, Human - ultrastructure ; Cytogenetics ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. 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A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for electron microscopy (EM). Well-defined bands, symmetry of sister chromatids, concordance between homologues, and band patterns similar to those observed by light microscopy facilitate chromosome identification and karyotyping. From prophase to late metaphase, chromosomes condense and bands appear to fuse. The fusion appears to be owing to chromatin reorganization. Our results underline the value of using immunogold reagents, which are ideal probes for antigen localization on chromosomes.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Bromodeoxyuridine - metabolism</subject><subject>Chromosome Banding - methods</subject><subject>Chromosomes - metabolism</subject><subject>Chromosomes - ultrastructure</subject><subject>Chromosomes, Human - metabolism</subject><subject>Chromosomes, Human - ultrastructure</subject><subject>Cytogenetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Horses - genetics</subject><subject>Human</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Microscopy, Electron - methods</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1Lw0AQhhdRaq3evAo5iCCYup_dzU0p9QMKXvS8bDabJmWTrbsNof_erQ315GmYmYd3Zt4B4BrBKUKcPxI-zaaYz4RA5ASMEWMoZZDSUzCGEOM0Fug5uAhhDSGilIkRGA34GDwtrNFb79qkqbV3QbvNLnFlsnK2SK3KjTVFUnWNahPVFon57urWJLryrnHBNSZcgrNS2WCuhjgBXy-Lz_lbuvx4fZ8_L1NNkdimDHGdMwWRFqgoEM8EIcLMSgxxmQtIykKVnBGmMMsU4SZmGWYFIzzTOdeCTMDdQXfj3XdnwlY2ddDGWtUa1wXJsyiKKY3gwwHcnxO8KeXG143yO4mg3BsmCZeZHByI-M2g2-WNKY7wX_926KuglS29anUdjhgnnMwgjtj9AQtqZeTadb6Nbvw3cjilqldVX3sjQ6OsjQsg2ff9LxsfRcgPl62LWA</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Messier, PE</creator><creator>Drouin, R</creator><creator>Richer, CL</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890901</creationdate><title>Electron microscopy of gold-labeled human and equine chromosomes</title><author>Messier, PE ; Drouin, R ; Richer, CL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-517cb5a01c81dd1798338e6f202fb803fdaf7535a259a37eaf7925d5379cb7c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Bromodeoxyuridine - metabolism</topic><topic>Chromosome Banding - methods</topic><topic>Chromosomes - metabolism</topic><topic>Chromosomes - ultrastructure</topic><topic>Chromosomes, Human - metabolism</topic><topic>Chromosomes, Human - ultrastructure</topic><topic>Cytogenetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Horses - genetics</topic><topic>Human</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Microscopy, Electron - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Messier, PE</creatorcontrib><creatorcontrib>Drouin, R</creatorcontrib><creatorcontrib>Richer, CL</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Messier, PE</au><au>Drouin, R</au><au>Richer, CL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electron microscopy of gold-labeled human and equine chromosomes</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>37</volume><issue>9</issue><spage>1443</spage><epage>1447</epage><pages>1443-1447</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>We present an immunochemical technique for the detection of 5-bromo-2'-deoxyuridine (BrdU) incorporated discontinuously into the chromosomal DNA. 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| ispartof | The journal of histochemistry and cytochemistry, 1989-09, Vol.37 (9), p.1443-1447 |
| issn | 0022-1554 1551-5044 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79179244 |
| source | MEDLINE; SAGE Complete A-Z List; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies, Monoclonal Biological and medical sciences Bromodeoxyuridine - metabolism Chromosome Banding - methods Chromosomes - metabolism Chromosomes - ultrastructure Chromosomes, Human - metabolism Chromosomes, Human - ultrastructure Cytogenetics Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Horses - genetics Human Humans Immunohistochemistry - methods Microscopy, Electron - methods |
| title | Electron microscopy of gold-labeled human and equine chromosomes |
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