LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin- embedded tissues
We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal de...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1989-09, Vol.37 (9), p.1363-1370 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Stathopoulos, E Naeve, GS Taylor, CR Epstein, AL |
| description | We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer. |
| doi_str_mv | 10.1177/37.9.2671152 |
| format | Article |
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Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/37.9.2671152</identifier><identifier>PMID: 2671152</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Carcinoma - diagnosis ; Carcinoma - metabolism ; Carcinoma - pathology ; Fluorescent Antibody Technique ; Formaldehyde ; Histological Techniques ; Humans ; Immunoenzyme Techniques ; Lymphoma - diagnosis ; Lymphoma - metabolism ; Lymphoma - pathology ; Mesoderm - cytology ; Mesoderm - metabolism ; Mesoderm - ultrastructure ; Mice ; Mice, Inbred BALB C ; Paraffin ; Precipitin Tests ; Soft Tissue Neoplasms - diagnosis ; Soft Tissue Neoplasms - metabolism ; Soft Tissue Neoplasms - pathology ; Vimentin - immunology ; Vimentin - metabolism</subject><ispartof>The journal of histochemistry and cytochemistry, 1989-09, Vol.37 (9), p.1363-1370</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-a676ef26a97f26bc83bb823abf8fd7c47f666918e20e4c625a828dfc5d20b7d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/37.9.2671152$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/37.9.2671152$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2671152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stathopoulos, E</creatorcontrib><creatorcontrib>Naeve, GS</creatorcontrib><creatorcontrib>Taylor, CR</creatorcontrib><creatorcontrib>Epstein, AL</creatorcontrib><title>LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin- embedded tissues</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Carcinoma - diagnosis</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - pathology</subject><subject>Fluorescent Antibody Technique</subject><subject>Formaldehyde</subject><subject>Histological Techniques</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Lymphoma - diagnosis</subject><subject>Lymphoma - metabolism</subject><subject>Lymphoma - pathology</subject><subject>Mesoderm - cytology</subject><subject>Mesoderm - metabolism</subject><subject>Mesoderm - ultrastructure</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Paraffin</subject><subject>Precipitin Tests</subject><subject>Soft Tissue Neoplasms - diagnosis</subject><subject>Soft Tissue Neoplasms - metabolism</subject><subject>Soft Tissue Neoplasms - pathology</subject><subject>Vimentin - immunology</subject><subject>Vimentin - metabolism</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU2PFCEQhonRrOPqzasJJxOTZQS6G7q9rRu_kole9ExoKHbYNE0L3Ts7_8afKuN09OQFUm-99RRUIfSS0S1jUr6t5LbbciEZa_gjtGFNw0hD6_ox2lDKOSlC_RQ9y_mOUlbXTXuBLlb7Bv3afSXiHdY4xDGaIY56wHqcfR_tEc8R3_sAJRwxPEwJcgaLSzDGkewh6DlO0cPsDQ6QYTT7Yyj1BoYhF4rFFpK_LyXzEmI6Swm0mYt4wrxviPMPYK_wpJN2zo8EQ-jB2lONz3mB_Bw9cXrI8GK9L9GPjx--33wmu2-fvtxc74ip2m4mWkgBjgvdyXL2pq36vuWV7l3rrDS1dEKIjrXAKdRG8Ea3vLXONJbTXlpWXaLXZ-6U4s_Sd1bB59NP9AhxyUp2TApZ02K8OhtNijkncGpKPuh0VIyq00JUJVWn1gkX-6uVu_QB7F_zv_ybcz7rW1B3cUllBfl_rPWNe3-7P_gEKpd5D4XM1OFw-ONllaiq38X7ohg</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Stathopoulos, E</creator><creator>Naeve, GS</creator><creator>Taylor, CR</creator><creator>Epstein, AL</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890901</creationdate><title>LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin- embedded tissues</title><author>Stathopoulos, E ; Naeve, GS ; Taylor, CR ; Epstein, AL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-a676ef26a97f26bc83bb823abf8fd7c47f666918e20e4c625a828dfc5d20b7d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Carcinoma - diagnosis</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - pathology</topic><topic>Fluorescent Antibody Technique</topic><topic>Formaldehyde</topic><topic>Histological Techniques</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Lymphoma - diagnosis</topic><topic>Lymphoma - metabolism</topic><topic>Lymphoma - pathology</topic><topic>Mesoderm - cytology</topic><topic>Mesoderm - metabolism</topic><topic>Mesoderm - ultrastructure</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Paraffin</topic><topic>Precipitin Tests</topic><topic>Soft Tissue Neoplasms - diagnosis</topic><topic>Soft Tissue Neoplasms - metabolism</topic><topic>Soft Tissue Neoplasms - pathology</topic><topic>Vimentin - immunology</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stathopoulos, E</creatorcontrib><creatorcontrib>Naeve, GS</creatorcontrib><creatorcontrib>Taylor, CR</creatorcontrib><creatorcontrib>Epstein, AL</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stathopoulos, E</au><au>Naeve, GS</au><au>Taylor, CR</au><au>Epstein, AL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin- embedded tissues</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>37</volume><issue>9</issue><spage>1363</spage><epage>1370</epage><pages>1363-1370</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>We generated a monoclonal antibody (MAb), designated LN-6, directed against human vimentin, which retains its immunoreactivity in B5-fixed, paraffin-embedded tissues. Like other anti-vimentin MAb, LN-6 was found to be reactive with a wide spectrum of human sarcomas and normal cells of mesenchymal derivation. However, unlike other similar reagents, LN-6 was unreactive with normal and malignant human lymphoid cells and therefore displays a more restricted immunoreactivity. Because of its ability to stain routinely processed pathological tissues and its marked reactivity with human sarcomas, LN-6 is a unique reagent for the immunohistochemical diagnosis of human cancer.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>2671152</pmid><doi>10.1177/37.9.2671152</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1554 |
| ispartof | The journal of histochemistry and cytochemistry, 1989-09, Vol.37 (9), p.1363-1370 |
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| source | Access via SAGE; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies, Monoclonal - immunology Carcinoma - diagnosis Carcinoma - metabolism Carcinoma - pathology Fluorescent Antibody Technique Formaldehyde Histological Techniques Humans Immunoenzyme Techniques Lymphoma - diagnosis Lymphoma - metabolism Lymphoma - pathology Mesoderm - cytology Mesoderm - metabolism Mesoderm - ultrastructure Mice Mice, Inbred BALB C Paraffin Precipitin Tests Soft Tissue Neoplasms - diagnosis Soft Tissue Neoplasms - metabolism Soft Tissue Neoplasms - pathology Vimentin - immunology Vimentin - metabolism |
| title | LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin- embedded tissues |
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