Distribution of cytopathic and noncytopathic bovine viral diarrhea virus antigens in tissues of calves following acute experimental infection
Institute of Microbiology, Federal University of Rio de Janeiro, Brazil. The distribution of cytopathic and noncytopathic biotypes of bovine viral diarrhea virus (BVDV) in the tissues of colostrum-fed and colostrum-deprived calves was investigated. Colostrum-fed (group A) and colostrum-deprived (gro...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 1997-07, Vol.9 (3), p.287-297 |
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| creator | Spagnuolo-Weaver, M. (Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.) Allan, G.M Kennedy, S Foster, J.C Adair, B.M |
| description | Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.
The distribution of cytopathic and noncytopathic biotypes of bovine viral diarrhea virus (BVDV) in the tissues of colostrum-fed and colostrum-deprived calves was investigated. Colostrum-fed (group A) and colostrum-deprived (group B) calves were experimentally infected with the BVDV isolate 80/1, which contains both BVDV biotypes. Colostrum-deprived calves were also experimentally infected with a noncytopathic BVDV (group C) or with a cytopathic BVDV (group D) cloned from the 80/1 isolate. All calves were sequentially euthanized, and a wide range of tissue samples were processed for immunofluorescent and virus isolation studies. In group A, consistent immunofluorescent staining for BVDV was detected in vascular smooth muscle of numerous blood vessels in the tissues examined, mainly at 11 and 13 days postinoculation. A predominance of samples containing cytopathic BVDV was observed in the calves of this group, following virus isolation studies. Both cytopathic and noncytopathic BVDV were detected/recovered from a larger range of specimens in the calves in group B than from the calves in group A. In the calves in all the experimental groups, large amounts of BVDV antigen were detected mainly in tissue samples from the lymphoid and gastrointestinal systems, whereas only minimal amounts of BVDV were detected in the respiratory tract. Abundant noncytopathic BVDV antigen was also detected in pituitary gland and in Langerhans islets in pancreases of colostrum-deprived calves infected with the cloned noncytopathic BVDV. Noncytopathic BVDV was isolated from a wider range of tissues from calves in group C than in the colostrum-deprived calves infected with both BVDV biotypes. A cytopathic BVDV was isolated/detected in retropharyngeal, mesenteric, and abomasal lymph nodes and in thymus of 2 calves in group C. Cytopathic BVDV was detected/isolated mainly from mesenteric lymph nodes and Peyer's patches of the calves in group D. |
| doi_str_mv | 10.1177/104063879700900310 |
| format | Article |
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The distribution of cytopathic and noncytopathic biotypes of bovine viral diarrhea virus (BVDV) in the tissues of colostrum-fed and colostrum-deprived calves was investigated. Colostrum-fed (group A) and colostrum-deprived (group B) calves were experimentally infected with the BVDV isolate 80/1, which contains both BVDV biotypes. Colostrum-deprived calves were also experimentally infected with a noncytopathic BVDV (group C) or with a cytopathic BVDV (group D) cloned from the 80/1 isolate. All calves were sequentially euthanized, and a wide range of tissue samples were processed for immunofluorescent and virus isolation studies. In group A, consistent immunofluorescent staining for BVDV was detected in vascular smooth muscle of numerous blood vessels in the tissues examined, mainly at 11 and 13 days postinoculation. A predominance of samples containing cytopathic BVDV was observed in the calves of this group, following virus isolation studies. Both cytopathic and noncytopathic BVDV were detected/recovered from a larger range of specimens in the calves in group B than from the calves in group A. In the calves in all the experimental groups, large amounts of BVDV antigen were detected mainly in tissue samples from the lymphoid and gastrointestinal systems, whereas only minimal amounts of BVDV were detected in the respiratory tract. Abundant noncytopathic BVDV antigen was also detected in pituitary gland and in Langerhans islets in pancreases of colostrum-deprived calves infected with the cloned noncytopathic BVDV. Noncytopathic BVDV was isolated from a wider range of tissues from calves in group C than in the colostrum-deprived calves infected with both BVDV biotypes. A cytopathic BVDV was isolated/detected in retropharyngeal, mesenteric, and abomasal lymph nodes and in thymus of 2 calves in group C. Cytopathic BVDV was detected/isolated mainly from mesenteric lymph nodes and Peyer's patches of the calves in group D.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063879700900310</identifier><identifier>PMID: 9249168</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>ANIMAL TISSUES ; Animals ; Antibodies, Viral - blood ; ANTIGENE ; ANTIGENOS ; ANTIGENS ; Antigens, Viral - analysis ; BIOTIPOS ; BIOTYPE ; BIOTYPES ; BOVINE DIARRHOEA PESTIVIRUS ; Bovine Virus Diarrhea-Mucosal Disease - diagnosis ; Bovine Virus Diarrhea-Mucosal Disease - immunology ; Bovine Virus Diarrhea-Mucosal Disease - pathology ; CALOSTRO ; CALVES ; Cattle ; COLOSTRUM ; COWS ; Digestive System - pathology ; Digestive System - virology ; EXPERIMENTACION IN VIVO ; EXPERIMENTAL INFECTION ; EXPERIMENTATION IN VIVO ; Female ; Fluorescent Antibody Technique ; Immunohistochemistry ; IN VIVO EXPERIMENTATION ; INFECCION EXPERIMENTAL ; INFECTION EXPERIMENTALE ; Lymphoid Tissue - pathology ; Lymphoid Tissue - virology ; Muscle, Smooth, Vascular - virology ; Pestivirus - isolation & purification ; Pestivirus - pathogenicity ; PESTIVIRUS DE LA DIARREA BOVINA ; PESTIVIRUS MALADIE DES MUQUEUSES ; TEJIDOS ANIMALES ; TERNERO ; TISSU ANIMAL ; VACA ; VACHE ; VEAU ; VIRAL ANTIGENS</subject><ispartof>Journal of veterinary diagnostic investigation, 1997-07, Vol.9 (3), p.287-297</ispartof><rights>1997 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-fbf1e51a7b2c1062bffd1bc59f6b088b3504c0f281cc608bb3521e6f81b972833</citedby><cites>FETCH-LOGICAL-c431t-fbf1e51a7b2c1062bffd1bc59f6b088b3504c0f281cc608bb3521e6f81b972833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9249168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spagnuolo-Weaver, M. (Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.)</creatorcontrib><creatorcontrib>Allan, G.M</creatorcontrib><creatorcontrib>Kennedy, S</creatorcontrib><creatorcontrib>Foster, J.C</creatorcontrib><creatorcontrib>Adair, B.M</creatorcontrib><title>Distribution of cytopathic and noncytopathic bovine viral diarrhea virus antigens in tissues of calves following acute experimental infection</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.
The distribution of cytopathic and noncytopathic biotypes of bovine viral diarrhea virus (BVDV) in the tissues of colostrum-fed and colostrum-deprived calves was investigated. Colostrum-fed (group A) and colostrum-deprived (group B) calves were experimentally infected with the BVDV isolate 80/1, which contains both BVDV biotypes. Colostrum-deprived calves were also experimentally infected with a noncytopathic BVDV (group C) or with a cytopathic BVDV (group D) cloned from the 80/1 isolate. All calves were sequentially euthanized, and a wide range of tissue samples were processed for immunofluorescent and virus isolation studies. In group A, consistent immunofluorescent staining for BVDV was detected in vascular smooth muscle of numerous blood vessels in the tissues examined, mainly at 11 and 13 days postinoculation. A predominance of samples containing cytopathic BVDV was observed in the calves of this group, following virus isolation studies. Both cytopathic and noncytopathic BVDV were detected/recovered from a larger range of specimens in the calves in group B than from the calves in group A. In the calves in all the experimental groups, large amounts of BVDV antigen were detected mainly in tissue samples from the lymphoid and gastrointestinal systems, whereas only minimal amounts of BVDV were detected in the respiratory tract. Abundant noncytopathic BVDV antigen was also detected in pituitary gland and in Langerhans islets in pancreases of colostrum-deprived calves infected with the cloned noncytopathic BVDV. Noncytopathic BVDV was isolated from a wider range of tissues from calves in group C than in the colostrum-deprived calves infected with both BVDV biotypes. A cytopathic BVDV was isolated/detected in retropharyngeal, mesenteric, and abomasal lymph nodes and in thymus of 2 calves in group C. Cytopathic BVDV was detected/isolated mainly from mesenteric lymph nodes and Peyer's patches of the calves in group D.</description><subject>ANIMAL TISSUES</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Antigens, Viral - analysis</subject><subject>BIOTIPOS</subject><subject>BIOTYPE</subject><subject>BIOTYPES</subject><subject>BOVINE DIARRHOEA PESTIVIRUS</subject><subject>Bovine Virus Diarrhea-Mucosal Disease - diagnosis</subject><subject>Bovine Virus Diarrhea-Mucosal Disease - immunology</subject><subject>Bovine Virus Diarrhea-Mucosal Disease - pathology</subject><subject>CALOSTRO</subject><subject>CALVES</subject><subject>Cattle</subject><subject>COLOSTRUM</subject><subject>COWS</subject><subject>Digestive System - pathology</subject><subject>Digestive System - virology</subject><subject>EXPERIMENTACION IN VIVO</subject><subject>EXPERIMENTAL INFECTION</subject><subject>EXPERIMENTATION IN VIVO</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Immunohistochemistry</subject><subject>IN VIVO EXPERIMENTATION</subject><subject>INFECCION EXPERIMENTAL</subject><subject>INFECTION EXPERIMENTALE</subject><subject>Lymphoid Tissue - pathology</subject><subject>Lymphoid Tissue - virology</subject><subject>Muscle, Smooth, Vascular - virology</subject><subject>Pestivirus - isolation & purification</subject><subject>Pestivirus - pathogenicity</subject><subject>PESTIVIRUS DE LA DIARREA BOVINA</subject><subject>PESTIVIRUS MALADIE DES MUQUEUSES</subject><subject>TEJIDOS ANIMALES</subject><subject>TERNERO</subject><subject>TISSU ANIMAL</subject><subject>VACA</subject><subject>VACHE</subject><subject>VEAU</subject><subject>VIRAL ANTIGENS</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kdFuFCEUhidGU9vqCzQx4UbvxnJgZoBLU7WaNPFCe02AgVk2s7ACs7UP4TvLOhtjYuIVh3O-_88Pp2muAL8FYOwacIcHyplgGAuMKeAnzTmIjradoMPTWlegPRLPm4uctxj3pGdw1pwJ0gkY-Hnz873PJXm9FB8Dig6ZxxL3qmy8QSqMKMTwV0fHgw8WHXxSMxq9Smlj1fG65EoXP9mQkQ-o-JwXm3_7qflQKxfnOT74MCFllmKR_bG3ye9sKNXJB2fNMcCL5plTc7YvT-dlc__xw7ebT-3dl9vPN-_uWtNRKK3TDmwPimliAA9EOzeCNr1wg8aca9rjzmBHOBgzYK5rg4AdHActGOGUXjZvVt99it9r0CJ3Phs7zyrYuGTJBLBOCF5BsoImxZyTdXJfU6v0KAHL4w7kvzuoolcn90Xv7PhHcvr0Or9e51lNVm7jkkJ97P8dX6-KjZ82Dz5ZmXdqnqs_kdvD6IWkknBWuauVcypKNSWf5f1XwboeeEd_ASDiqDU</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Spagnuolo-Weaver, M. (Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.)</creator><creator>Allan, G.M</creator><creator>Kennedy, S</creator><creator>Foster, J.C</creator><creator>Adair, B.M</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970701</creationdate><title>Distribution of cytopathic and noncytopathic bovine viral diarrhea virus antigens in tissues of calves following acute experimental infection</title><author>Spagnuolo-Weaver, M. (Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.) ; Allan, G.M ; Kennedy, S ; Foster, J.C ; Adair, B.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-fbf1e51a7b2c1062bffd1bc59f6b088b3504c0f281cc608bb3521e6f81b972833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ANIMAL TISSUES</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Antigens, Viral - analysis</topic><topic>BIOTIPOS</topic><topic>BIOTYPE</topic><topic>BIOTYPES</topic><topic>BOVINE DIARRHOEA PESTIVIRUS</topic><topic>Bovine Virus Diarrhea-Mucosal Disease - diagnosis</topic><topic>Bovine Virus Diarrhea-Mucosal Disease - immunology</topic><topic>Bovine Virus Diarrhea-Mucosal Disease - pathology</topic><topic>CALOSTRO</topic><topic>CALVES</topic><topic>Cattle</topic><topic>COLOSTRUM</topic><topic>COWS</topic><topic>Digestive System - pathology</topic><topic>Digestive System - virology</topic><topic>EXPERIMENTACION IN VIVO</topic><topic>EXPERIMENTAL INFECTION</topic><topic>EXPERIMENTATION IN VIVO</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Immunohistochemistry</topic><topic>IN VIVO EXPERIMENTATION</topic><topic>INFECCION EXPERIMENTAL</topic><topic>INFECTION EXPERIMENTALE</topic><topic>Lymphoid Tissue - pathology</topic><topic>Lymphoid Tissue - virology</topic><topic>Muscle, Smooth, Vascular - virology</topic><topic>Pestivirus - isolation & purification</topic><topic>Pestivirus - pathogenicity</topic><topic>PESTIVIRUS DE LA DIARREA BOVINA</topic><topic>PESTIVIRUS MALADIE DES MUQUEUSES</topic><topic>TEJIDOS ANIMALES</topic><topic>TERNERO</topic><topic>TISSU ANIMAL</topic><topic>VACA</topic><topic>VACHE</topic><topic>VEAU</topic><topic>VIRAL ANTIGENS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Spagnuolo-Weaver, M. (Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.)</creatorcontrib><creatorcontrib>Allan, G.M</creatorcontrib><creatorcontrib>Kennedy, S</creatorcontrib><creatorcontrib>Foster, J.C</creatorcontrib><creatorcontrib>Adair, B.M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Spagnuolo-Weaver, M. (Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.)</au><au>Allan, G.M</au><au>Kennedy, S</au><au>Foster, J.C</au><au>Adair, B.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of cytopathic and noncytopathic bovine viral diarrhea virus antigens in tissues of calves following acute experimental infection</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>9</volume><issue>3</issue><spage>287</spage><epage>297</epage><pages>287-297</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Institute of Microbiology, Federal University of Rio de Janeiro, Brazil.
The distribution of cytopathic and noncytopathic biotypes of bovine viral diarrhea virus (BVDV) in the tissues of colostrum-fed and colostrum-deprived calves was investigated. Colostrum-fed (group A) and colostrum-deprived (group B) calves were experimentally infected with the BVDV isolate 80/1, which contains both BVDV biotypes. Colostrum-deprived calves were also experimentally infected with a noncytopathic BVDV (group C) or with a cytopathic BVDV (group D) cloned from the 80/1 isolate. All calves were sequentially euthanized, and a wide range of tissue samples were processed for immunofluorescent and virus isolation studies. In group A, consistent immunofluorescent staining for BVDV was detected in vascular smooth muscle of numerous blood vessels in the tissues examined, mainly at 11 and 13 days postinoculation. A predominance of samples containing cytopathic BVDV was observed in the calves of this group, following virus isolation studies. Both cytopathic and noncytopathic BVDV were detected/recovered from a larger range of specimens in the calves in group B than from the calves in group A. In the calves in all the experimental groups, large amounts of BVDV antigen were detected mainly in tissue samples from the lymphoid and gastrointestinal systems, whereas only minimal amounts of BVDV were detected in the respiratory tract. Abundant noncytopathic BVDV antigen was also detected in pituitary gland and in Langerhans islets in pancreases of colostrum-deprived calves infected with the cloned noncytopathic BVDV. Noncytopathic BVDV was isolated from a wider range of tissues from calves in group C than in the colostrum-deprived calves infected with both BVDV biotypes. A cytopathic BVDV was isolated/detected in retropharyngeal, mesenteric, and abomasal lymph nodes and in thymus of 2 calves in group C. Cytopathic BVDV was detected/isolated mainly from mesenteric lymph nodes and Peyer's patches of the calves in group D.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>9249168</pmid><doi>10.1177/104063879700900310</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of veterinary diagnostic investigation, 1997-07, Vol.9 (3), p.287-297 |
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| language | eng |
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| subjects | ANIMAL TISSUES Animals Antibodies, Viral - blood ANTIGENE ANTIGENOS ANTIGENS Antigens, Viral - analysis BIOTIPOS BIOTYPE BIOTYPES BOVINE DIARRHOEA PESTIVIRUS Bovine Virus Diarrhea-Mucosal Disease - diagnosis Bovine Virus Diarrhea-Mucosal Disease - immunology Bovine Virus Diarrhea-Mucosal Disease - pathology CALOSTRO CALVES Cattle COLOSTRUM COWS Digestive System - pathology Digestive System - virology EXPERIMENTACION IN VIVO EXPERIMENTAL INFECTION EXPERIMENTATION IN VIVO Female Fluorescent Antibody Technique Immunohistochemistry IN VIVO EXPERIMENTATION INFECCION EXPERIMENTAL INFECTION EXPERIMENTALE Lymphoid Tissue - pathology Lymphoid Tissue - virology Muscle, Smooth, Vascular - virology Pestivirus - isolation & purification Pestivirus - pathogenicity PESTIVIRUS DE LA DIARREA BOVINA PESTIVIRUS MALADIE DES MUQUEUSES TEJIDOS ANIMALES TERNERO TISSU ANIMAL VACA VACHE VEAU VIRAL ANTIGENS |
| title | Distribution of cytopathic and noncytopathic bovine viral diarrhea virus antigens in tissues of calves following acute experimental infection |
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