Growth-promoting effects of acidic and basic fibroblast growth factor on rabbit articular chondrocytes aging in culture
Rabbit articular chondrocytes have a limited growth potential in vitro. After four passages in culture, chondrocytes have accomplished more than 50% of their life span. At this stage of culture, they are considered to be senescent-like, since a dramatic decrease in proliferative capacity and enhance...
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| Veröffentlicht in: | Experimental cell research 1989-08, Vol.183 (2), p.388-398 |
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| creator | Froger-Gaillard, B. Charrier, A.M. Thenet, S. Ronot, X. Adolphe, M. |
| description | Rabbit articular chondrocytes have a limited growth potential
in vitro. After four passages in culture, chondrocytes have accomplished more than 50% of their life span. At this stage of culture, they are considered to be senescent-like, since a dramatic decrease in proliferative capacity and enhanced cell size and protein content are observed. These aged cells are, however, still able to respond to fibroblast growth factor (FGF). The addition of either acidic or basic FGF (10 ng/ml) to culture medium permitted an enhanced proliferation. The attenuation of FGF mitogenic activity during aging was not observed for both fractions. Moreover, when treated with acidic or basic FGF, aged chondrocytes had a smaller size and a lower protein content. The acidic FGF was less potent than the basic FGF in delaying the evolution of aged chondrocytes to senescence. |
| doi_str_mv | 10.1016/0014-4827(89)90399-6 |
| format | Article |
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The acidic FGF was less potent than the basic FGF in delaying the evolution of aged chondrocytes to senescence.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Culture Techniques - methods</subject><subject>DNA - biosynthesis</subject><subject>DNA Replication - drug effects</subject><subject>Fibroblast Growth Factors - isolation & purification</subject><subject>Fibroblast Growth Factors - pharmacology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. 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| subjects | Animals Biological and medical sciences Cartilage, Articular - cytology Cartilage, Articular - drug effects Cell Division - drug effects Cell physiology Cells, Cultured Culture Techniques - methods DNA - biosynthesis DNA Replication - drug effects Fibroblast Growth Factors - isolation & purification Fibroblast Growth Factors - pharmacology Flow Cytometry Fundamental and applied biological sciences. Psychology Molecular and cellular biology Rabbits Responses to growth factors, tumor promotors, other factors Thymidine - metabolism |
| title | Growth-promoting effects of acidic and basic fibroblast growth factor on rabbit articular chondrocytes aging in culture |
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