Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms
Serial flow cytometric nuclear DNA content analyses were performed from normal human spleen, thyroid, liver, and pancreas removed from ten patients at autopsy and stored for up to 8 d without any preservative to study the effect of autolysis on DNA histograms. Fine needle aspiration biopsy (FNAB) sa...
Gespeichert in:
| Veröffentlicht in: | Cytometry (New York, N.Y.) N.Y.), 1989-07, Vol.10 (4), p.417-425 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 425 |
|---|---|
| container_issue | 4 |
| container_start_page | 417 |
| container_title | Cytometry (New York, N.Y.) |
| container_volume | 10 |
| creator | Alanen, Kalle A. Joensuu, Heikki Klemi, Pekka J. |
| description | Serial flow cytometric nuclear DNA content analyses were performed from normal human spleen, thyroid, liver, and pancreas removed from ten patients at autopsy and stored for up to 8 d without any preservative to study the effect of autolysis on DNA histograms. Fine needle aspiration biopsy (FNAB) samples were taken in diluted ethanol and tissue biopsies from the same area in formalin for embedding into paraffin at the time of autopsy and serially thereafter. Histograms obtained from samples taken within 10 h after death had a symmetrical G1 peak with a small coefficient of variance (CV) except histograms produced from paraffin‐embedded pancreatic tissue, but bimodal distributions similar to those seen in aneuploid tumors were obtained from many samples stored longer than for 20 h. The DNA indices of the bimodal histograms were usually |
| doi_str_mv | 10.1002/cyto.990100409 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79172083</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79172083</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5369-9c49b6fef47ba823a5e1f6cfad4cc777a5953276bddcb614b7ca482082cbc6aa3</originalsourceid><addsrcrecordid>eNqFkM1LxDAQxYMoun5cvQm56K1r0qZJc1zWTxC96EEvZZomGk03NWmR_e_NuosehcAQ5jdv3jyEjimZUkLyc7Uc_FRKkj6MyC00oUSKjBQ52UYTQiXPmODFHtqP8Z0QIjkrdtFuLjivqmqCXmbj4N0y2ojTA9z7QS8GCw5HPwalsTfYgIsaw0KPvfO2xb2Gj4QvsHH-C68MdHoIVuGL-xl-s3HwrwG6eIh2fiaPNvUAPV1dPs5vsruH69v57C5TZcFlJhWTDTfaMNFAlRdQamq4MtAypYQQUMqySH6btlUNp6wRCliVkypXjeIAxQE6W-v2wX-OOg51Z6PSziXHfoy1kFQkvPgXpCVnXOQygdM1qIKPMWhT98F2EJY1JfUq9Xp1dP2beho42SiPTafbX3wTc-qfbvoQFTgTYKFs_FOVrKo4o4mTa-7LOr38Z2s9f358-PPwDdI7nUQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15646729</pqid></control><display><type>article</type><title>Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Alanen, Kalle A. ; Joensuu, Heikki ; Klemi, Pekka J.</creator><creatorcontrib>Alanen, Kalle A. ; Joensuu, Heikki ; Klemi, Pekka J.</creatorcontrib><description>Serial flow cytometric nuclear DNA content analyses were performed from normal human spleen, thyroid, liver, and pancreas removed from ten patients at autopsy and stored for up to 8 d without any preservative to study the effect of autolysis on DNA histograms. Fine needle aspiration biopsy (FNAB) samples were taken in diluted ethanol and tissue biopsies from the same area in formalin for embedding into paraffin at the time of autopsy and serially thereafter. Histograms obtained from samples taken within 10 h after death had a symmetrical G1 peak with a small coefficient of variance (CV) except histograms produced from paraffin‐embedded pancreatic tissue, but bimodal distributions similar to those seen in aneuploid tumors were obtained from many samples stored longer than for 20 h. The DNA indices of the bimodal histograms were usually <1.3. The false peaks were more prominent in FNAB samples than in paraffin‐embedded samples. The time of appearance of the false aneuploid peaks varied individually, and they were usually first seen in samples taken from the pancreas, followed by the liver, the thyroid and the spleen. Because neoplasms may become necrotic in vivo and fixation of fresh surgical samples may be slow and incomplete, increased DNA staining caused by autolysis may be a source of false aneuploid peaks in DNA content analysis.</description><identifier>ISSN: 0196-4763</identifier><identifier>EISSN: 1097-0320</identifier><identifier>DOI: 10.1002/cyto.990100409</identifier><identifier>PMID: 2766888</identifier><identifier>CODEN: CYTODQ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Biopsy, Needle ; Coefficient of variation ; DNA - analysis ; DNA index ; Dna, deoxyribonucleoproteins ; ethanol preservation ; False Positive Reactions ; Flow Cytometry - methods ; Fundamental and applied biological sciences. Psychology ; Humans ; Liver - analysis ; Liver - cytology ; Nucleic acids ; Pancreas - analysis ; Pancreas - cytology ; paraffin‐embedded samples ; Ploidies ; serial analysis ; Spleen - analysis ; Spleen - cytology ; Thyroid Gland - analysis ; Thyroid Gland - cytology</subject><ispartof>Cytometry (New York, N.Y.), 1989-07, Vol.10 (4), p.417-425</ispartof><rights>Copyright © 1989 Wiley‐Liss, Inc.</rights><rights>1991 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5369-9c49b6fef47ba823a5e1f6cfad4cc777a5953276bddcb614b7ca482082cbc6aa3</citedby><cites>FETCH-LOGICAL-c5369-9c49b6fef47ba823a5e1f6cfad4cc777a5953276bddcb614b7ca482082cbc6aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19488641$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2766888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alanen, Kalle A.</creatorcontrib><creatorcontrib>Joensuu, Heikki</creatorcontrib><creatorcontrib>Klemi, Pekka J.</creatorcontrib><title>Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms</title><title>Cytometry (New York, N.Y.)</title><addtitle>Cytometry</addtitle><description>Serial flow cytometric nuclear DNA content analyses were performed from normal human spleen, thyroid, liver, and pancreas removed from ten patients at autopsy and stored for up to 8 d without any preservative to study the effect of autolysis on DNA histograms. Fine needle aspiration biopsy (FNAB) samples were taken in diluted ethanol and tissue biopsies from the same area in formalin for embedding into paraffin at the time of autopsy and serially thereafter. Histograms obtained from samples taken within 10 h after death had a symmetrical G1 peak with a small coefficient of variance (CV) except histograms produced from paraffin‐embedded pancreatic tissue, but bimodal distributions similar to those seen in aneuploid tumors were obtained from many samples stored longer than for 20 h. The DNA indices of the bimodal histograms were usually <1.3. The false peaks were more prominent in FNAB samples than in paraffin‐embedded samples. The time of appearance of the false aneuploid peaks varied individually, and they were usually first seen in samples taken from the pancreas, followed by the liver, the thyroid and the spleen. Because neoplasms may become necrotic in vivo and fixation of fresh surgical samples may be slow and incomplete, increased DNA staining caused by autolysis may be a source of false aneuploid peaks in DNA content analysis.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biopsy, Needle</subject><subject>Coefficient of variation</subject><subject>DNA - analysis</subject><subject>DNA index</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>ethanol preservation</subject><subject>False Positive Reactions</subject><subject>Flow Cytometry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Liver - analysis</subject><subject>Liver - cytology</subject><subject>Nucleic acids</subject><subject>Pancreas - analysis</subject><subject>Pancreas - cytology</subject><subject>paraffin‐embedded samples</subject><subject>Ploidies</subject><subject>serial analysis</subject><subject>Spleen - analysis</subject><subject>Spleen - cytology</subject><subject>Thyroid Gland - analysis</subject><subject>Thyroid Gland - cytology</subject><issn>0196-4763</issn><issn>1097-0320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LxDAQxYMoun5cvQm56K1r0qZJc1zWTxC96EEvZZomGk03NWmR_e_NuosehcAQ5jdv3jyEjimZUkLyc7Uc_FRKkj6MyC00oUSKjBQ52UYTQiXPmODFHtqP8Z0QIjkrdtFuLjivqmqCXmbj4N0y2ojTA9z7QS8GCw5HPwalsTfYgIsaw0KPvfO2xb2Gj4QvsHH-C68MdHoIVuGL-xl-s3HwrwG6eIh2fiaPNvUAPV1dPs5vsruH69v57C5TZcFlJhWTDTfaMNFAlRdQamq4MtAypYQQUMqySH6btlUNp6wRCliVkypXjeIAxQE6W-v2wX-OOg51Z6PSziXHfoy1kFQkvPgXpCVnXOQygdM1qIKPMWhT98F2EJY1JfUq9Xp1dP2beho42SiPTafbX3wTc-qfbvoQFTgTYKFs_FOVrKo4o4mTa-7LOr38Z2s9f358-PPwDdI7nUQ</recordid><startdate>198907</startdate><enddate>198907</enddate><creator>Alanen, Kalle A.</creator><creator>Joensuu, Heikki</creator><creator>Klemi, Pekka J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>198907</creationdate><title>Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms</title><author>Alanen, Kalle A. ; Joensuu, Heikki ; Klemi, Pekka J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5369-9c49b6fef47ba823a5e1f6cfad4cc777a5953276bddcb614b7ca482082cbc6aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biopsy, Needle</topic><topic>Coefficient of variation</topic><topic>DNA - analysis</topic><topic>DNA index</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>ethanol preservation</topic><topic>False Positive Reactions</topic><topic>Flow Cytometry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Liver - analysis</topic><topic>Liver - cytology</topic><topic>Nucleic acids</topic><topic>Pancreas - analysis</topic><topic>Pancreas - cytology</topic><topic>paraffin‐embedded samples</topic><topic>Ploidies</topic><topic>serial analysis</topic><topic>Spleen - analysis</topic><topic>Spleen - cytology</topic><topic>Thyroid Gland - analysis</topic><topic>Thyroid Gland - cytology</topic><toplevel>online_resources</toplevel><creatorcontrib>Alanen, Kalle A.</creatorcontrib><creatorcontrib>Joensuu, Heikki</creatorcontrib><creatorcontrib>Klemi, Pekka J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alanen, Kalle A.</au><au>Joensuu, Heikki</au><au>Klemi, Pekka J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms</atitle><jtitle>Cytometry (New York, N.Y.)</jtitle><addtitle>Cytometry</addtitle><date>1989-07</date><risdate>1989</risdate><volume>10</volume><issue>4</issue><spage>417</spage><epage>425</epage><pages>417-425</pages><issn>0196-4763</issn><eissn>1097-0320</eissn><coden>CYTODQ</coden><abstract>Serial flow cytometric nuclear DNA content analyses were performed from normal human spleen, thyroid, liver, and pancreas removed from ten patients at autopsy and stored for up to 8 d without any preservative to study the effect of autolysis on DNA histograms. Fine needle aspiration biopsy (FNAB) samples were taken in diluted ethanol and tissue biopsies from the same area in formalin for embedding into paraffin at the time of autopsy and serially thereafter. Histograms obtained from samples taken within 10 h after death had a symmetrical G1 peak with a small coefficient of variance (CV) except histograms produced from paraffin‐embedded pancreatic tissue, but bimodal distributions similar to those seen in aneuploid tumors were obtained from many samples stored longer than for 20 h. The DNA indices of the bimodal histograms were usually <1.3. The false peaks were more prominent in FNAB samples than in paraffin‐embedded samples. The time of appearance of the false aneuploid peaks varied individually, and they were usually first seen in samples taken from the pancreas, followed by the liver, the thyroid and the spleen. Because neoplasms may become necrotic in vivo and fixation of fresh surgical samples may be slow and incomplete, increased DNA staining caused by autolysis may be a source of false aneuploid peaks in DNA content analysis.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2766888</pmid><doi>10.1002/cyto.990100409</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-4763 |
| ispartof | Cytometry (New York, N.Y.), 1989-07, Vol.10 (4), p.417-425 |
| issn | 0196-4763 1097-0320 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79172083 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Biopsy, Needle Coefficient of variation DNA - analysis DNA index Dna, deoxyribonucleoproteins ethanol preservation False Positive Reactions Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Humans Liver - analysis Liver - cytology Nucleic acids Pancreas - analysis Pancreas - cytology paraffin‐embedded samples Ploidies serial analysis Spleen - analysis Spleen - cytology Thyroid Gland - analysis Thyroid Gland - cytology |
| title | Autolysis is a potential source of false aneuploid peaks in flow cytometric DNA histograms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T19%3A07%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autolysis%20is%20a%20potential%20source%20of%20false%20aneuploid%20peaks%20in%20flow%20cytometric%20DNA%20histograms&rft.jtitle=Cytometry%20(New%20York,%20N.Y.)&rft.au=Alanen,%20Kalle%20A.&rft.date=1989-07&rft.volume=10&rft.issue=4&rft.spage=417&rft.epage=425&rft.pages=417-425&rft.issn=0196-4763&rft.eissn=1097-0320&rft.coden=CYTODQ&rft_id=info:doi/10.1002/cyto.990100409&rft_dat=%3Cproquest_cross%3E79172083%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15646729&rft_id=info:pmid/2766888&rfr_iscdi=true |