The bioenergetics of mitochondria after cryopreservation
The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me 2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay...
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| Veröffentlicht in: | Cryobiology 1989-08, Vol.26 (4), p.333-340 |
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| container_end_page | 340 |
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| container_title | Cryobiology |
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| creator | Fuller, Barry J. Rubinacci, Alessandro Geboes, Karel De Loecker, William |
| description | The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me
2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me
2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me
2SO effected some changes in mitochondrial function. |
| doi_str_mv | 10.1016/0011-2240(89)90057-6 |
| format | Article |
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2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me
2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me
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2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me
2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me
2SO effected some changes in mitochondrial function.</description><subject>Adenosine Triphosphate - biosynthesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell metabolism, cell oxidation</subject><subject>Cell physiology</subject><subject>Dimethyl Sulfoxide</subject><subject>Energy Metabolism</subject><subject>Freezing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Oxygen Consumption</subject><subject>Rats</subject><subject>Tissue Preservation</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcIeRPSwmmx283ERpPgFBS_1HLLJxEa2m5psC_33bu3So5eZwzzvy_AgdEnwPcGEPWBMSF4UJb4V8k5iXPGcHaExwRLnBZXFMRofkFN0ltI3xphxWo7QqOCMcUHGSMwXkNU-QAvxCzpvUhZctvRdMIvQ2uh1pl0HMTNxG1YREsSN7nxoz9GJ002Ci2FP0OfL83z6ls8-Xt-nT7PcUMG6XBtNeV05UhtLCRWVrCQUxJSOgK04ZaICYnXF-1X0s7bScSG5qy3vCyidoJt97yqGnzWkTi19MtA0uoWwTopLwgSRogfLPWhiSCmCU6volzpuFcFqJ0ztbKidDSWk-hOmWB-7GvrX9RLsITQY6u_Xw10noxsXdWt8OmBMMorLssce9xj0LjYeokrGQ2vA-gimUzb4___4BYb_hkE</recordid><startdate>19890801</startdate><enddate>19890801</enddate><creator>Fuller, Barry J.</creator><creator>Rubinacci, Alessandro</creator><creator>Geboes, Karel</creator><creator>De Loecker, William</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890801</creationdate><title>The bioenergetics of mitochondria after cryopreservation</title><author>Fuller, Barry J. ; Rubinacci, Alessandro ; Geboes, Karel ; De Loecker, William</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-aca37b5f1bcd31385959e21c4f1ed573685e1da575e12575bd9f7897fbd738633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adenosine Triphosphate - biosynthesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell metabolism, cell oxidation</topic><topic>Cell physiology</topic><topic>Dimethyl Sulfoxide</topic><topic>Energy Metabolism</topic><topic>Freezing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Oxygen Consumption</topic><topic>Rats</topic><topic>Tissue Preservation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fuller, Barry J.</creatorcontrib><creatorcontrib>Rubinacci, Alessandro</creatorcontrib><creatorcontrib>Geboes, Karel</creatorcontrib><creatorcontrib>De Loecker, William</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fuller, Barry J.</au><au>Rubinacci, Alessandro</au><au>Geboes, Karel</au><au>De Loecker, William</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The bioenergetics of mitochondria after cryopreservation</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>1989-08-01</date><risdate>1989</risdate><volume>26</volume><issue>4</issue><spage>333</spage><epage>340</epage><pages>333-340</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><coden>CRYBAS</coden><abstract>The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me
2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me
2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me
2SO effected some changes in mitochondrial function.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2766781</pmid><doi>10.1016/0011-2240(89)90057-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | Cryobiology, 1989-08, Vol.26 (4), p.333-340 |
| issn | 0011-2240 1090-2392 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - biosynthesis Animals Biological and medical sciences Cell metabolism, cell oxidation Cell physiology Dimethyl Sulfoxide Energy Metabolism Freezing Fundamental and applied biological sciences. Psychology In Vitro Techniques Mitochondria, Liver - metabolism Molecular and cellular biology Oxygen Consumption Rats Tissue Preservation |
| title | The bioenergetics of mitochondria after cryopreservation |
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