Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1
We previously reported two oncogenic point mutations present in the RB (retinoblastoma) gene promoter region, found at consensus Sp1 and ATF sites, respectively, and in two separate hereditary RB families. However, Sp1 protein was shown not to bind to the Sp1 site; this indicated that the Sp1 consen...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1997-08, Vol.57 (15), p.3145-3148 |
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| creator | SOWA, Y SHIIO, Y HANDA, H SAKAI, T FUJITA, T MATSUMOTO, T OKUYAMA, Y KATO, D INOUE, J.-I SAWADA, J.-I GOTO, M WATANABE, H |
| description | We previously reported two oncogenic point mutations present in the RB (retinoblastoma) gene promoter region, found at consensus Sp1 and ATF sites, respectively, and in two separate hereditary RB families. However, Sp1 protein was shown not to bind to the Sp1 site; this indicated that the Sp1 consensus site mutation was blocking the action of an alternative transcription factor, which we called RBF-1 (retinoblastoma binding factor 1). Subsequent purification of RBF-1 revealed it to be hGABP/E4TF1, a transactivator from the adenovirus early-region 4 promoter. In this study, we directly examined the effects of hGABP/E4TF1 on transactivation of the RB gene promoter through the RBF-1 site. As expected, hGABP/E4TF1 enhanced the core RB promoter activity, whereas it did not stimulate a mutant RBF-1 site. We therefore conclude that the most essential transcription factor in the human RB gene is likely to be hGABP/E4TF1. |
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However, Sp1 protein was shown not to bind to the Sp1 site; this indicated that the Sp1 consensus site mutation was blocking the action of an alternative transcription factor, which we called RBF-1 (retinoblastoma binding factor 1). Subsequent purification of RBF-1 revealed it to be hGABP/E4TF1, a transactivator from the adenovirus early-region 4 promoter. In this study, we directly examined the effects of hGABP/E4TF1 on transactivation of the RB gene promoter through the RBF-1 site. As expected, hGABP/E4TF1 enhanced the core RB promoter activity, whereas it did not stimulate a mutant RBF-1 site. We therefore conclude that the most essential transcription factor in the human RB gene is likely to be hGABP/E4TF1.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 9242441</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Biological and medical sciences ; Carrier Proteins - genetics ; DNA-Binding Proteins - metabolism ; Drosophila ; Fundamental and applied biological sciences. Psychology ; GA-Binding Protein Transcription Factor ; Genes, Reporter ; Genes, Retinoblastoma - genetics ; Humans ; Molecular and cellular biology ; Molecular genetics ; Point Mutation ; Promoter Regions, Genetic - physiology ; Transcription Factors - metabolism ; Transcription. Transcription factor. Splicing. Rna processing ; Transfection</subject><ispartof>Cancer research (Chicago, Ill.), 1997-08, Vol.57 (15), p.3145-3148</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2794737$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9242441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOWA, Y</creatorcontrib><creatorcontrib>SHIIO, Y</creatorcontrib><creatorcontrib>HANDA, H</creatorcontrib><creatorcontrib>SAKAI, T</creatorcontrib><creatorcontrib>FUJITA, T</creatorcontrib><creatorcontrib>MATSUMOTO, T</creatorcontrib><creatorcontrib>OKUYAMA, Y</creatorcontrib><creatorcontrib>KATO, D</creatorcontrib><creatorcontrib>INOUE, J.-I</creatorcontrib><creatorcontrib>SAWADA, J.-I</creatorcontrib><creatorcontrib>GOTO, M</creatorcontrib><creatorcontrib>WATANABE, H</creatorcontrib><title>Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>We previously reported two oncogenic point mutations present in the RB (retinoblastoma) gene promoter region, found at consensus Sp1 and ATF sites, respectively, and in two separate hereditary RB families. However, Sp1 protein was shown not to bind to the Sp1 site; this indicated that the Sp1 consensus site mutation was blocking the action of an alternative transcription factor, which we called RBF-1 (retinoblastoma binding factor 1). Subsequent purification of RBF-1 revealed it to be hGABP/E4TF1, a transactivator from the adenovirus early-region 4 promoter. In this study, we directly examined the effects of hGABP/E4TF1 on transactivation of the RB gene promoter through the RBF-1 site. As expected, hGABP/E4TF1 enhanced the core RB promoter activity, whereas it did not stimulate a mutant RBF-1 site. We therefore conclude that the most essential transcription factor in the human RB gene is likely to be hGABP/E4TF1.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Drosophila</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GA-Binding Protein Transcription Factor</subject><subject>Genes, Reporter</subject><subject>Genes, Retinoblastoma - genetics</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Point Mutation</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>GA-Binding Protein Transcription Factor</topic><topic>Genes, Reporter</topic><topic>Genes, Retinoblastoma - genetics</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Point Mutation</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription. Transcription factor. Splicing. 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However, Sp1 protein was shown not to bind to the Sp1 site; this indicated that the Sp1 consensus site mutation was blocking the action of an alternative transcription factor, which we called RBF-1 (retinoblastoma binding factor 1). Subsequent purification of RBF-1 revealed it to be hGABP/E4TF1, a transactivator from the adenovirus early-region 4 promoter. In this study, we directly examined the effects of hGABP/E4TF1 on transactivation of the RB gene promoter through the RBF-1 site. As expected, hGABP/E4TF1 enhanced the core RB promoter activity, whereas it did not stimulate a mutant RBF-1 site. We therefore conclude that the most essential transcription factor in the human RB gene is likely to be hGABP/E4TF1.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>9242441</pmid><tpages>4</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Carrier Proteins - genetics DNA-Binding Proteins - metabolism Drosophila Fundamental and applied biological sciences. Psychology GA-Binding Protein Transcription Factor Genes, Reporter Genes, Retinoblastoma - genetics Humans Molecular and cellular biology Molecular genetics Point Mutation Promoter Regions, Genetic - physiology Transcription Factors - metabolism Transcription. Transcription factor. Splicing. Rna processing Transfection |
| title | Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1 |
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