Infectious Barley stripe mosaic virus RNA transcribed in Vitro from full-length genomic cDNA clones

Full-length genomic cDNA clones of the Type and ND18 strains of barley stripe mosaic virus (BSMV) were transcribed in vitro using T7 RNA polymerase. The combination of RNAs α, β, and γ synthesized in the presence of 5′ cap analogs was infectious after inoculation onto barley plants, conclusively dem...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1989-08, Vol.171 (2), p.342-349
Hauptverfasser:	Petty, I.T.D., Hunter, B.G., Wei, N., Jackson, A.O.
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ARN Biological and medical sciences Blotting, Western CHENOPODIUM CHENOPODIUM AMARANTICOLOR CLONE CLONES Cloning, Molecular DNA DNA - genetics Edible Grain - microbiology EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO FENOTIPOS Fundamental and applied biological sciences. Psychology GENOMAS GENOME GENOMES Hordeum - microbiology HORDEUM VULGARE IN VITRO EXPERIMENTATION Microbiology Mosaic Viruses - genetics NICOTIANA PHENOTYPE PHENOTYPES Plant Diseases PLANT VIRUSES Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA RNA Caps RNA POLYMERASE RNA, Viral - genetics Transcription, Genetic TRANSFERASAS TRANSFERASE TRANSFERASES Viral Proteins - analysis Virology VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Virus Replication
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