Bioluminescence spectral and fluorescence dynamics study of the interaction of lumazine protein with the intermediates of bacterial luciferase bioluminescence

The mechanism of the shifting of the bioluminescence spectrum from the reaction of bacterial luciferase by lumazine protein is investigated by methods of fluorescence dynamics. A metastable intermediate is produced on reaction of Vibrio harveyi luciferase with FMNH2 and O2. It has an absorption maxi...

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Veröffentlicht in:	Biochemistry (Easton) 1989-05, Vol.28 (10), p.4263-4271
Hauptverfasser:	Lee, John, O'Kane, Dennis J, Gibson, Bruce G
Format:	Artikel
Sprache:	eng
Schlagworte:	alkanal monooxygenase (FMN-linked) Bacterial Proteins - metabolism Carrier Proteins - metabolism Energy Transfer Luciferases - metabolism lumazine Luminescent Measurements Luminescent Proteins Photobacterium - metabolism Photobacterium phosphoreum Spectrometry, Fluorescence
Online-Zugang:	Volltext
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