Protective immunity induced by rotavirus DNA vaccines
It is estimated that Group A rotavirus diarrhea causes as many as one million deaths per year in children worldwide, and effective vaccines will be essential for their control. Plasmid DNA vaccines encoding murine rotaviral proteins VP4, VP6, or VP7 were tested in adult BALB/c mice for their ability...
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| Veröffentlicht in: | Vaccine 1997-06, Vol.15 (8), p.899-902 |
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| container_title | Vaccine |
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| creator | Chen, Shing C. Fynan, Ellen F. Robinson, Harriet L. Lu, Shan Greenberg, Harry B. Santoro, Joseph C. Herrmann, John E. |
| description | It is estimated that Group A rotavirus diarrhea causes as many as one million deaths per year in children worldwide, and effective vaccines will be essential for their control. Plasmid DNA vaccines encoding murine rotaviral proteins VP4, VP6, or VP7 were tested in adult BALB/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. The vaccines were administered by inoculation into cells of the epidermis with an Accell gene gun (Auragen, Inc., Middleton, WI, USA). Each vaccine elicited rotavirus-specific serum antibodies as measured by ELISA. Virus neutralizing antibodies were detected in mice receiving plasmid DNAs encoding for outer capsid proteins VP4 and VP7, but not for VP6, an inner capsid protein, and all of the vaccines generated virus-specific CTL responses. Each vaccine was effective in protecting mice against infection after homotypic rotavirus (100 ID
50) challenge, showing reductions (
P |
| doi_str_mv | 10.1016/S0264-410X(96)00272-1 |
| format | Article |
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50) challenge, showing reductions (
P<0.0002) in viral excretion measured over a 9 day period. Increased rotavirus-specific intestinal IgA antibodies were seen in vaccinated mice after rotavirus challenge, particularly in mice that received the VP6 DNA vaccine. This suggests that intracellular IgA-mediated neutralization may be involved in protective immunity induced by the VP6 DNA vaccine, and may represent a new mechanism for protection by DNA vaccines.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(96)00272-1</identifier><identifier>PMID: 9234543</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Viral - biosynthesis ; Antigens, Viral ; Applied microbiology ; Biolistics ; Biological and medical sciences ; Capsid - genetics ; Capsid - immunology ; Capsid Proteins ; DNA vaccine ; DNA, Viral - immunology ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Hemagglutinins, Viral - genetics ; Hemagglutinins, Viral - immunology ; Immunity ; Mice ; Mice, Inbred BALB C ; Microbiology ; Plasmids - genetics ; Promoter Regions, Genetic ; rotavirus ; Rotavirus - genetics ; Rotavirus - immunology ; Rotavirus Infections - immunology ; Rotavirus Infections - prevention & control ; rotaviruses ; T-Lymphocytes, Cytotoxic - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Virology</subject><ispartof>Vaccine, 1997-06, Vol.15 (8), p.899-902</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-fc967964c961c983148a06d124ff9bea45220de77678a4d89b2b7f6c5d2e48023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X96002721$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2755163$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9234543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Shing C.</creatorcontrib><creatorcontrib>Fynan, Ellen F.</creatorcontrib><creatorcontrib>Robinson, Harriet L.</creatorcontrib><creatorcontrib>Lu, Shan</creatorcontrib><creatorcontrib>Greenberg, Harry B.</creatorcontrib><creatorcontrib>Santoro, Joseph C.</creatorcontrib><creatorcontrib>Herrmann, John E.</creatorcontrib><title>Protective immunity induced by rotavirus DNA vaccines</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>It is estimated that Group A rotavirus diarrhea causes as many as one million deaths per year in children worldwide, and effective vaccines will be essential for their control. Plasmid DNA vaccines encoding murine rotaviral proteins VP4, VP6, or VP7 were tested in adult BALB/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. The vaccines were administered by inoculation into cells of the epidermis with an Accell gene gun (Auragen, Inc., Middleton, WI, USA). Each vaccine elicited rotavirus-specific serum antibodies as measured by ELISA. Virus neutralizing antibodies were detected in mice receiving plasmid DNAs encoding for outer capsid proteins VP4 and VP7, but not for VP6, an inner capsid protein, and all of the vaccines generated virus-specific CTL responses. Each vaccine was effective in protecting mice against infection after homotypic rotavirus (100 ID
50) challenge, showing reductions (
P<0.0002) in viral excretion measured over a 9 day period. Increased rotavirus-specific intestinal IgA antibodies were seen in vaccinated mice after rotavirus challenge, particularly in mice that received the VP6 DNA vaccine. This suggests that intracellular IgA-mediated neutralization may be involved in protective immunity induced by the VP6 DNA vaccine, and may represent a new mechanism for protection by DNA vaccines.</description><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antigens, Viral</subject><subject>Applied microbiology</subject><subject>Biolistics</subject><subject>Biological and medical sciences</subject><subject>Capsid - genetics</subject><subject>Capsid - immunology</subject><subject>Capsid Proteins</subject><subject>DNA vaccine</subject><subject>DNA, Viral - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutinins, Viral - genetics</subject><subject>Hemagglutinins, Viral - immunology</subject><subject>Immunity</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Plasmids - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>rotavirus</subject><subject>Rotavirus - genetics</subject><subject>Rotavirus - immunology</subject><subject>Rotavirus Infections - immunology</subject><subject>Rotavirus Infections - prevention & control</subject><subject>rotaviruses</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LwzAYx4Moc04_wqAHET1UkzSvJxnzFYYKKngLaZpCZG1n0hb27c22sutOz-H_e174PQBMEbxFELG7T4gZSQmCP9eS3UCIOU7RERgjwbMUUySOwXiPnIKzEH4hhDRDcgRGEmeEkmwM6IdvWmta19vEVVVXu3aduLrojC2SfJ3EVPfOdyF5eJslvTbG1Tacg5NSL4O9GOoEfD89fs1f0sX78-t8tkgNRbRNSyMZl4zEgowUGSJCQ1YgTMpS5lYTijEsLOeMC00KIXOc85IZWmBLBMTZBFzt5q5889fZ0KrKBWOXS13bpguKS8SgZPAgGLHIUhFBugONb0LwtlQr7yrt1wpBtfGqtl7VRpqSTG29KhT7psOCLq9sse8aRMb8csh1MHpZel0bF_YY5pQitsHud5iN1npnvQrG2TrKdj5-QRWNO3DIP7Rskts</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Chen, Shing C.</creator><creator>Fynan, Ellen F.</creator><creator>Robinson, Harriet L.</creator><creator>Lu, Shan</creator><creator>Greenberg, Harry B.</creator><creator>Santoro, Joseph C.</creator><creator>Herrmann, John E.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970601</creationdate><title>Protective immunity induced by rotavirus DNA vaccines</title><author>Chen, Shing C. ; Fynan, Ellen F. ; Robinson, Harriet L. ; Lu, Shan ; Greenberg, Harry B. ; Santoro, Joseph C. ; Herrmann, John E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-fc967964c961c983148a06d124ff9bea45220de77678a4d89b2b7f6c5d2e48023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antigens, Viral</topic><topic>Applied microbiology</topic><topic>Biolistics</topic><topic>Biological and medical sciences</topic><topic>Capsid - genetics</topic><topic>Capsid - immunology</topic><topic>Capsid Proteins</topic><topic>DNA vaccine</topic><topic>DNA, Viral - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemagglutinins, Viral - genetics</topic><topic>Hemagglutinins, Viral - immunology</topic><topic>Immunity</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Plasmids - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>rotavirus</topic><topic>Rotavirus - genetics</topic><topic>Rotavirus - immunology</topic><topic>Rotavirus Infections - immunology</topic><topic>Rotavirus Infections - prevention & control</topic><topic>rotaviruses</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shing C.</creatorcontrib><creatorcontrib>Fynan, Ellen F.</creatorcontrib><creatorcontrib>Robinson, Harriet L.</creatorcontrib><creatorcontrib>Lu, Shan</creatorcontrib><creatorcontrib>Greenberg, Harry B.</creatorcontrib><creatorcontrib>Santoro, Joseph C.</creatorcontrib><creatorcontrib>Herrmann, John E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shing C.</au><au>Fynan, Ellen F.</au><au>Robinson, Harriet L.</au><au>Lu, Shan</au><au>Greenberg, Harry B.</au><au>Santoro, Joseph C.</au><au>Herrmann, John E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective immunity induced by rotavirus DNA vaccines</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>15</volume><issue>8</issue><spage>899</spage><epage>902</epage><pages>899-902</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>It is estimated that Group A rotavirus diarrhea causes as many as one million deaths per year in children worldwide, and effective vaccines will be essential for their control. Plasmid DNA vaccines encoding murine rotaviral proteins VP4, VP6, or VP7 were tested in adult BALB/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. The vaccines were administered by inoculation into cells of the epidermis with an Accell gene gun (Auragen, Inc., Middleton, WI, USA). Each vaccine elicited rotavirus-specific serum antibodies as measured by ELISA. Virus neutralizing antibodies were detected in mice receiving plasmid DNAs encoding for outer capsid proteins VP4 and VP7, but not for VP6, an inner capsid protein, and all of the vaccines generated virus-specific CTL responses. Each vaccine was effective in protecting mice against infection after homotypic rotavirus (100 ID
50) challenge, showing reductions (
P<0.0002) in viral excretion measured over a 9 day period. Increased rotavirus-specific intestinal IgA antibodies were seen in vaccinated mice after rotavirus challenge, particularly in mice that received the VP6 DNA vaccine. This suggests that intracellular IgA-mediated neutralization may be involved in protective immunity induced by the VP6 DNA vaccine, and may represent a new mechanism for protection by DNA vaccines.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9234543</pmid><doi>10.1016/S0264-410X(96)00272-1</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 1997-06, Vol.15 (8), p.899-902 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antibodies, Viral - biosynthesis Antigens, Viral Applied microbiology Biolistics Biological and medical sciences Capsid - genetics Capsid - immunology Capsid Proteins DNA vaccine DNA, Viral - immunology Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Hemagglutinins, Viral - genetics Hemagglutinins, Viral - immunology Immunity Mice Mice, Inbred BALB C Microbiology Plasmids - genetics Promoter Regions, Genetic rotavirus Rotavirus - genetics Rotavirus - immunology Rotavirus Infections - immunology Rotavirus Infections - prevention & control rotaviruses T-Lymphocytes, Cytotoxic - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - administration & dosage Vaccines, DNA - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology Virology |
| title | Protective immunity induced by rotavirus DNA vaccines |
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