The development of porcine zona pellucida using monoclonal antibodies: II. Electron microscopy
Following our previous study on the immunohistochemistry of porcine zonae pellucidae (ZP), we undertook the present study to localize the components of the ZP with immunoelectron microscopy, using three types of anti-porcine-ZP monoclonal antibodies (Mabs), named STA-1, STA-2, and STA-3. Some organe...
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| Veröffentlicht in: | Biology of reproduction 1989-05, Vol.40 (5), p.1103-1108 |
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| container_title | Biology of reproduction |
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| creator | TAKAGI, J DOBASHI, M ARAKI, Y IMAI, Y HIROI, M TONOSAKI, A SENDO, F |
| description | Following our previous study on the immunohistochemistry of porcine zonae pellucidae (ZP), we undertook the present study
to localize the components of the ZP with immunoelectron microscopy, using three types of anti-porcine-ZP monoclonal antibodies
(Mabs), named STA-1, STA-2, and STA-3. Some organelles of the oocyte were seen to react with STA-2 and STA-3 prior to ZP formation.
As soon as a follicle began to mature, STA-2 and STA-3 reacted with the perinuclear space and the endoplasmic reticular membrane
of the oocyte. The follicle first reacted with STA-1 at the secondary follicle stage. At this stage, the positive reaction
involved the follicular cell layer as well as the oocyte and ZP. Positive reaction was scattered within and limited to the
interfollicular cell space and was never found in the cytoplasm of follicular cells. At the antral follicle stage, the oocyte
was surrounded by a thick, electron-dense ZP. A strong reaction was observed in the outer layer, but no significant reaction
occurred in the inner layer. The convex and ragged outer margin of the ZP was characterized by the strongest reaction. |
| doi_str_mv | 10.1095/biolreprod40.5.1103 |
| format | Article |
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to localize the components of the ZP with immunoelectron microscopy, using three types of anti-porcine-ZP monoclonal antibodies
(Mabs), named STA-1, STA-2, and STA-3. Some organelles of the oocyte were seen to react with STA-2 and STA-3 prior to ZP formation.
As soon as a follicle began to mature, STA-2 and STA-3 reacted with the perinuclear space and the endoplasmic reticular membrane
of the oocyte. The follicle first reacted with STA-1 at the secondary follicle stage. At this stage, the positive reaction
involved the follicular cell layer as well as the oocyte and ZP. Positive reaction was scattered within and limited to the
interfollicular cell space and was never found in the cytoplasm of follicular cells. At the antral follicle stage, the oocyte
was surrounded by a thick, electron-dense ZP. A strong reaction was observed in the outer layer, but no significant reaction
occurred in the inner layer. The convex and ragged outer margin of the ZP was characterized by the strongest reaction.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod40.5.1103</identifier><identifier>PMID: 2765613</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Mammalian female genital system ; Microscopy, Electron ; Morphology. Physiology ; Oocytes - physiology ; Ovary - physiology ; Ovum - physiology ; Swine ; Vertebrates: reproduction ; Zona Pellucida - immunology ; Zona Pellucida - physiology ; Zona Pellucida - ultrastructure</subject><ispartof>Biology of reproduction, 1989-05, Vol.40 (5), p.1103-1108</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3213-6bc62b30367063242b0091183df08687229f37f9f6b9e1c6489ae873f37acb7e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19292577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2765613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TAKAGI, J</creatorcontrib><creatorcontrib>DOBASHI, M</creatorcontrib><creatorcontrib>ARAKI, Y</creatorcontrib><creatorcontrib>IMAI, Y</creatorcontrib><creatorcontrib>HIROI, M</creatorcontrib><creatorcontrib>TONOSAKI, A</creatorcontrib><creatorcontrib>SENDO, F</creatorcontrib><title>The development of porcine zona pellucida using monoclonal antibodies: II. Electron microscopy</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Following our previous study on the immunohistochemistry of porcine zonae pellucidae (ZP), we undertook the present study
to localize the components of the ZP with immunoelectron microscopy, using three types of anti-porcine-ZP monoclonal antibodies
(Mabs), named STA-1, STA-2, and STA-3. Some organelles of the oocyte were seen to react with STA-2 and STA-3 prior to ZP formation.
As soon as a follicle began to mature, STA-2 and STA-3 reacted with the perinuclear space and the endoplasmic reticular membrane
of the oocyte. The follicle first reacted with STA-1 at the secondary follicle stage. At this stage, the positive reaction
involved the follicular cell layer as well as the oocyte and ZP. Positive reaction was scattered within and limited to the
interfollicular cell space and was never found in the cytoplasm of follicular cells. At the antral follicle stage, the oocyte
was surrounded by a thick, electron-dense ZP. A strong reaction was observed in the outer layer, but no significant reaction
occurred in the inner layer. The convex and ragged outer margin of the ZP was characterized by the strongest reaction.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Mammalian female genital system</subject><subject>Microscopy, Electron</subject><subject>Morphology. Physiology</subject><subject>Oocytes - physiology</subject><subject>Ovary - physiology</subject><subject>Ovum - physiology</subject><subject>Swine</subject><subject>Vertebrates: reproduction</subject><subject>Zona Pellucida - immunology</subject><subject>Zona Pellucida - physiology</subject><subject>Zona Pellucida - ultrastructure</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEFr3DAQhUVpSDdpf0Ep6NLevJE0tmT1VkLaLAR6Sa4VsjzOqsiSK62zpL8-XrJ0exqY9-bN4yPkI2drznRz1fkUMk459TVbN2vOGbwhK94IXSkh27dkxRiTFYCEd-SilN-M8RoEnJNzoWQjOazIr_st0h6fMKRpxLijaaBTys5HpH9TtHTCEGbne0vn4uMjHVNMLixKoDbufJd6j-Ur3WzW9Cag2-UU6ehdTsWl6fk9ORtsKPjhOC_Jw_eb--vb6u7nj831t7vKgeBQyc5J0QEDqZgEUYuOMc15C_3AWtkqIfQAatCD7DRyJ-tWW2wVLEvrOoVwSb685i40_sxYdmb0xS3VbcQ0F6M0l6yt1WKEV-OhYck4mCn70eZnw5k5UDX_UzWNOVBdrj4d4-duxP7fzRHjon8-6rY4G4Zso_PlFK2FFo1Sp5pb_7jd-4ymjDaEJRXMfr8_PXwBfWCQCQ</recordid><startdate>198905</startdate><enddate>198905</enddate><creator>TAKAGI, J</creator><creator>DOBASHI, M</creator><creator>ARAKI, Y</creator><creator>IMAI, Y</creator><creator>HIROI, M</creator><creator>TONOSAKI, A</creator><creator>SENDO, F</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198905</creationdate><title>The development of porcine zona pellucida using monoclonal antibodies: II. Electron microscopy</title><author>TAKAGI, J ; DOBASHI, M ; ARAKI, Y ; IMAI, Y ; HIROI, M ; TONOSAKI, A ; SENDO, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3213-6bc62b30367063242b0091183df08687229f37f9f6b9e1c6489ae873f37acb7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Mammalian female genital system</topic><topic>Microscopy, Electron</topic><topic>Morphology. Physiology</topic><topic>Oocytes - physiology</topic><topic>Ovary - physiology</topic><topic>Ovum - physiology</topic><topic>Swine</topic><topic>Vertebrates: reproduction</topic><topic>Zona Pellucida - immunology</topic><topic>Zona Pellucida - physiology</topic><topic>Zona Pellucida - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAKAGI, J</creatorcontrib><creatorcontrib>DOBASHI, M</creatorcontrib><creatorcontrib>ARAKI, Y</creatorcontrib><creatorcontrib>IMAI, Y</creatorcontrib><creatorcontrib>HIROI, M</creatorcontrib><creatorcontrib>TONOSAKI, A</creatorcontrib><creatorcontrib>SENDO, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAKAGI, J</au><au>DOBASHI, M</au><au>ARAKI, Y</au><au>IMAI, Y</au><au>HIROI, M</au><au>TONOSAKI, A</au><au>SENDO, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The development of porcine zona pellucida using monoclonal antibodies: II. Electron microscopy</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1989-05</date><risdate>1989</risdate><volume>40</volume><issue>5</issue><spage>1103</spage><epage>1108</epage><pages>1103-1108</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Following our previous study on the immunohistochemistry of porcine zonae pellucidae (ZP), we undertook the present study
to localize the components of the ZP with immunoelectron microscopy, using three types of anti-porcine-ZP monoclonal antibodies
(Mabs), named STA-1, STA-2, and STA-3. Some organelles of the oocyte were seen to react with STA-2 and STA-3 prior to ZP formation.
As soon as a follicle began to mature, STA-2 and STA-3 reacted with the perinuclear space and the endoplasmic reticular membrane
of the oocyte. The follicle first reacted with STA-1 at the secondary follicle stage. At this stage, the positive reaction
involved the follicular cell layer as well as the oocyte and ZP. Positive reaction was scattered within and limited to the
interfollicular cell space and was never found in the cytoplasm of follicular cells. At the antral follicle stage, the oocyte
was surrounded by a thick, electron-dense ZP. A strong reaction was observed in the outer layer, but no significant reaction
occurred in the inner layer. The convex and ragged outer margin of the ZP was characterized by the strongest reaction.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>2765613</pmid><doi>10.1095/biolreprod40.5.1103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biology of reproduction, 1989-05, Vol.40 (5), p.1103-1108 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79160847 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antibodies, Monoclonal Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Immunohistochemistry Mammalian female genital system Microscopy, Electron Morphology. Physiology Oocytes - physiology Ovary - physiology Ovum - physiology Swine Vertebrates: reproduction Zona Pellucida - immunology Zona Pellucida - physiology Zona Pellucida - ultrastructure |
| title | The development of porcine zona pellucida using monoclonal antibodies: II. Electron microscopy |
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