Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex
BALB/c and C57BL/6 mice were injected intramuscularly with plasmid DNA encoding the three components of the immunodominant 30–32 kDa antigen 85 complex (Ag85A, Ag85B, and Ag85C) from Mycobacterium tuberculosis culture filtrate, in order to investigate the utility of nucleic acid vaccination for indu...
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| Veröffentlicht in: | Vaccine 1997-06, Vol.15 (8), p.830-833 |
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| creator | Lozes, Evelyne Huygen, Kris Content, Jean Denis, Olivier Montgomery, Donna L. Yawman, Anne M. Vandenbussche, Paul Van Vooren, Jean-Paul Drowart, Annie Ulmer, Jeffrey B. Liu, Margaret A. |
| description | BALB/c and C57BL/6 mice were injected intramuscularly with plasmid DNA encoding the three components of the immunodominant 30–32 kDa antigen 85 complex (Ag85A, Ag85B, and Ag85C) from
Mycobacterium tuberculosis culture filtrate, in order to investigate the utility of nucleic acid vaccination for induction of immune responses against mycobacterial antigens. Ag85A and Ag85B encoding plasmids induced a robust Th1-like response towards native Ag85, characterized by elevated levels of interleukin (IL)-2, interferon-γ, and TNF-a. Levels of IL-4, IL-6, and IL-10 were low or undetectable. Plasmid encoding Ag85C was not effective. Cytotoxic T cell activity was also generated in
in vitro restimulated splenocyte cultures from Ag85A and Ag85B DNA vaccinated mice. Finally, Ag85A and Ag85B DNA vaccination conferred significant protection against mycobacterial replication in lungs from B6 mice, subsequently challenged. Therefore, this technique may be useful for the definition of protective antigens of
M. tuberculosis and the development of a more effective tuberculosis vaccine. |
| doi_str_mv | 10.1016/S0264-410X(96)00274-5 |
| format | Article |
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Mycobacterium tuberculosis culture filtrate, in order to investigate the utility of nucleic acid vaccination for induction of immune responses against mycobacterial antigens. Ag85A and Ag85B encoding plasmids induced a robust Th1-like response towards native Ag85, characterized by elevated levels of interleukin (IL)-2, interferon-γ, and TNF-a. Levels of IL-4, IL-6, and IL-10 were low or undetectable. Plasmid encoding Ag85C was not effective. Cytotoxic T cell activity was also generated in
in vitro restimulated splenocyte cultures from Ag85A and Ag85B DNA vaccinated mice. Finally, Ag85A and Ag85B DNA vaccination conferred significant protection against mycobacterial replication in lungs from B6 mice, subsequently challenged. Therefore, this technique may be useful for the definition of protective antigens of
M. tuberculosis and the development of a more effective tuberculosis vaccine.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(96)00274-5</identifier><identifier>PMID: 9234526</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>AIDS/HIV ; Animals ; Antibodies, Bacterial - biosynthesis ; antigen 85 ; Antigens, Bacterial - genetics ; Antigens, Bacterial - immunology ; Applied microbiology ; Bacteriology ; BCG Vaccine - administration & dosage ; BCG Vaccine - genetics ; BCG Vaccine - immunology ; Biological and medical sciences ; Cell Division - drug effects ; Cytokines - biosynthesis ; DNA vaccine ; DNA, Bacterial - immunology ; Fundamental and applied biological sciences. Psychology ; Injections, Intramuscular ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Microbiology ; Mycobacterium bovis - cytology ; Mycobacterium bovis - immunology ; Mycobacterium tuberculosis ; T-Lymphocytes, Cytotoxic - immunology ; Th1 Cells - immunology ; tuberculosis ; Tuberculosis - immunology ; Tuberculosis - prevention & control ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology</subject><ispartof>Vaccine, 1997-06, Vol.15 (8), p.830-833</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-cff8241006980a2e162cae7f3ba6523477ab06ea51ef11adabde5b21212f72003</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0264-410X(96)00274-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3548,23929,23930,25139,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2755141$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9234526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lozes, Evelyne</creatorcontrib><creatorcontrib>Huygen, Kris</creatorcontrib><creatorcontrib>Content, Jean</creatorcontrib><creatorcontrib>Denis, Olivier</creatorcontrib><creatorcontrib>Montgomery, Donna L.</creatorcontrib><creatorcontrib>Yawman, Anne M.</creatorcontrib><creatorcontrib>Vandenbussche, Paul</creatorcontrib><creatorcontrib>Van Vooren, Jean-Paul</creatorcontrib><creatorcontrib>Drowart, Annie</creatorcontrib><creatorcontrib>Ulmer, Jeffrey B.</creatorcontrib><creatorcontrib>Liu, Margaret A.</creatorcontrib><title>Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>BALB/c and C57BL/6 mice were injected intramuscularly with plasmid DNA encoding the three components of the immunodominant 30–32 kDa antigen 85 complex (Ag85A, Ag85B, and Ag85C) from
Mycobacterium tuberculosis culture filtrate, in order to investigate the utility of nucleic acid vaccination for induction of immune responses against mycobacterial antigens. Ag85A and Ag85B encoding plasmids induced a robust Th1-like response towards native Ag85, characterized by elevated levels of interleukin (IL)-2, interferon-γ, and TNF-a. Levels of IL-4, IL-6, and IL-10 were low or undetectable. Plasmid encoding Ag85C was not effective. Cytotoxic T cell activity was also generated in
in vitro restimulated splenocyte cultures from Ag85A and Ag85B DNA vaccinated mice. Finally, Ag85A and Ag85B DNA vaccination conferred significant protection against mycobacterial replication in lungs from B6 mice, subsequently challenged. Therefore, this technique may be useful for the definition of protective antigens of
M. tuberculosis and the development of a more effective tuberculosis vaccine.</description><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>antigen 85</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - immunology</subject><subject>Applied microbiology</subject><subject>Bacteriology</subject><subject>BCG Vaccine - administration & dosage</subject><subject>BCG Vaccine - genetics</subject><subject>BCG Vaccine - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cytokines - biosynthesis</subject><subject>DNA vaccine</subject><subject>DNA, Bacterial - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Injections, Intramuscular</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Mycobacterium bovis - cytology</subject><subject>Mycobacterium bovis - immunology</subject><subject>Mycobacterium tuberculosis</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Th1 Cells - immunology</subject><subject>tuberculosis</subject><subject>Tuberculosis - immunology</subject><subject>Tuberculosis - prevention & control</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v3CAQxVHUKtmk-QiROFRVe3AL2IB9qqK0-SNF6aGt1BvCeEiobNgCjrLfvnh3tdeIAxLze2-GeQhdUPKZEiq-_CRMNFVDyZ-PnfhECJNNxY_Qirayrhin7Ru0OiAn6DSlv4QQXtPuGB13rG44EyuU76Zp9uERvDMub7D2AwZrndFmg4PFGue5h2jmMSSX8LeHS_ysjXEeMHgTBucfcX4CbMK0Dh58TotqeUlgImQYimV2xR-3fEuN8PIOvbV6THC-v8_Q7-vvv65uq_sfN3dXl_eVaVqRK2Nty8r0RHQt0QyoYEaDtHWvBS8fkFL3RIDmFCyletD9ALxntBwrGSH1Gfqw813H8G-GlNXkkoFx1B7CnJTsqCBt3b0KFkzKRvIC8h1oYkgpglXr6CYdN4oStcSitrGoZeeqE2obi1p0F_sGcz_BcFDtcyj19_u6TkaPNmpvXDpgTHJOG1qwrzsMytaeHUSVjCs5wOAimKyG4F4Z5D-GoapC</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Lozes, Evelyne</creator><creator>Huygen, Kris</creator><creator>Content, Jean</creator><creator>Denis, Olivier</creator><creator>Montgomery, Donna L.</creator><creator>Yawman, Anne M.</creator><creator>Vandenbussche, Paul</creator><creator>Van Vooren, Jean-Paul</creator><creator>Drowart, Annie</creator><creator>Ulmer, Jeffrey B.</creator><creator>Liu, Margaret A.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19970601</creationdate><title>Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex</title><author>Lozes, Evelyne ; Huygen, Kris ; Content, Jean ; Denis, Olivier ; Montgomery, Donna L. ; Yawman, Anne M. ; Vandenbussche, Paul ; Van Vooren, Jean-Paul ; Drowart, Annie ; Ulmer, Jeffrey B. ; Liu, Margaret A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-cff8241006980a2e162cae7f3ba6523477ab06ea51ef11adabde5b21212f72003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Antibodies, Bacterial - biosynthesis</topic><topic>antigen 85</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Bacterial - immunology</topic><topic>Applied microbiology</topic><topic>Bacteriology</topic><topic>BCG Vaccine - administration & dosage</topic><topic>BCG Vaccine - genetics</topic><topic>BCG Vaccine - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cytokines - biosynthesis</topic><topic>DNA vaccine</topic><topic>DNA, Bacterial - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Injections, Intramuscular</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Mycobacterium bovis - cytology</topic><topic>Mycobacterium bovis - immunology</topic><topic>Mycobacterium tuberculosis</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Th1 Cells - immunology</topic><topic>tuberculosis</topic><topic>Tuberculosis - immunology</topic><topic>Tuberculosis - prevention & control</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lozes, Evelyne</creatorcontrib><creatorcontrib>Huygen, Kris</creatorcontrib><creatorcontrib>Content, Jean</creatorcontrib><creatorcontrib>Denis, Olivier</creatorcontrib><creatorcontrib>Montgomery, Donna L.</creatorcontrib><creatorcontrib>Yawman, Anne M.</creatorcontrib><creatorcontrib>Vandenbussche, Paul</creatorcontrib><creatorcontrib>Van Vooren, Jean-Paul</creatorcontrib><creatorcontrib>Drowart, Annie</creatorcontrib><creatorcontrib>Ulmer, Jeffrey B.</creatorcontrib><creatorcontrib>Liu, Margaret A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lozes, Evelyne</au><au>Huygen, Kris</au><au>Content, Jean</au><au>Denis, Olivier</au><au>Montgomery, Donna L.</au><au>Yawman, Anne M.</au><au>Vandenbussche, Paul</au><au>Van Vooren, Jean-Paul</au><au>Drowart, Annie</au><au>Ulmer, Jeffrey B.</au><au>Liu, Margaret A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>15</volume><issue>8</issue><spage>830</spage><epage>833</epage><pages>830-833</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>BALB/c and C57BL/6 mice were injected intramuscularly with plasmid DNA encoding the three components of the immunodominant 30–32 kDa antigen 85 complex (Ag85A, Ag85B, and Ag85C) from
Mycobacterium tuberculosis culture filtrate, in order to investigate the utility of nucleic acid vaccination for induction of immune responses against mycobacterial antigens. Ag85A and Ag85B encoding plasmids induced a robust Th1-like response towards native Ag85, characterized by elevated levels of interleukin (IL)-2, interferon-γ, and TNF-a. Levels of IL-4, IL-6, and IL-10 were low or undetectable. Plasmid encoding Ag85C was not effective. Cytotoxic T cell activity was also generated in
in vitro restimulated splenocyte cultures from Ag85A and Ag85B DNA vaccinated mice. Finally, Ag85A and Ag85B DNA vaccination conferred significant protection against mycobacterial replication in lungs from B6 mice, subsequently challenged. Therefore, this technique may be useful for the definition of protective antigens of
M. tuberculosis and the development of a more effective tuberculosis vaccine.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9234526</pmid><doi>10.1016/S0264-410X(96)00274-5</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 1997-06, Vol.15 (8), p.830-833 |
| issn | 0264-410X 1873-2518 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | AIDS/HIV Animals Antibodies, Bacterial - biosynthesis antigen 85 Antigens, Bacterial - genetics Antigens, Bacterial - immunology Applied microbiology Bacteriology BCG Vaccine - administration & dosage BCG Vaccine - genetics BCG Vaccine - immunology Biological and medical sciences Cell Division - drug effects Cytokines - biosynthesis DNA vaccine DNA, Bacterial - immunology Fundamental and applied biological sciences. Psychology Injections, Intramuscular Mice Mice, Inbred BALB C Mice, Inbred C57BL Microbiology Mycobacterium bovis - cytology Mycobacterium bovis - immunology Mycobacterium tuberculosis T-Lymphocytes, Cytotoxic - immunology Th1 Cells - immunology tuberculosis Tuberculosis - immunology Tuberculosis - prevention & control Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology |
| title | Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex |
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