Iris Pigment Epithelial Cells of Long Evans Rats Demonstrate Phagocytic Activity
The phagocytic activities of iris pigment epithelial (IPE) cells and retinal pigment epithelial (RPE) cells of Long Evans rats towards latex beads and rod outer segments (ROS) were compared in vitro. IPE and RPE cells of Long Evans rats were isolated and pure cultures obtained. The cultures were inc...
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| Veröffentlicht in: | Experimental eye research 1997-07, Vol.65 (1), p.23-29 |
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| container_title | Experimental eye research |
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| creator | REZAI, KOUROUS A LAPPAS, ALEXANDRA FARROKH-SIAR, LILI KOHEN, LEON WIEDEMANN, PETER HEIMANN, KLAUS |
| description | The phagocytic activities of iris pigment epithelial (IPE) cells and retinal pigment epithelial (RPE) cells of Long Evans rats towards latex beads and rod outer segments (ROS) were compared in vitro. IPE and RPE cells of Long Evans rats were isolated and pure cultures obtained. The cultures were incubated with latex beads, fixed, and analysed computer morphometrically, IPE and RPE cell cultures were also incubated with isolated ROS and examined using transmission electron microscopy. IPE cells were able to ingest latex beads. There was no significant difference between the number of latex particles phagocytized by IPE and RPE cells. After incubation with isolated ROS, IPE cells also recognized and ingested the ROS particles. However, the specific phagocytic capacity of IPE cells was 76% of that of RPE cells.
The autologous IPE cells might have the potential to be used as an alternative to RPE cells for transplantation in the subretinal space. |
| doi_str_mv | 10.1006/exer.1997.0307 |
| format | Article |
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The autologous IPE cells might have the potential to be used as an alternative to RPE cells for transplantation in the subretinal space.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1006/exer.1997.0307</identifier><identifier>PMID: 9237861</identifier><identifier>CODEN: EXERA6</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured - cytology ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Iris - cytology ; Iris - ultrastructure ; iris pigment epithelium ; latex ; Long Evans rats ; Microscopy, Electron ; morphometric analysis ; Phagocytosis ; Pigment Epithelium of Eye - cytology ; Rats ; retinal pigment epithelium ; Rod Cell Outer Segment ; rod outer segments ; Vertebrates: nervous system and sense organs</subject><ispartof>Experimental eye research, 1997-07, Vol.65 (1), p.23-29</ispartof><rights>1997 Academic Press</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-fa5235dc401733e17dce7a346e755678b8a08b5e19e875965fa5b9dab43fb1673</citedby><cites>FETCH-LOGICAL-c434t-fa5235dc401733e17dce7a346e755678b8a08b5e19e875965fa5b9dab43fb1673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/exer.1997.0307$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2811249$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9237861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>REZAI, KOUROUS A</creatorcontrib><creatorcontrib>LAPPAS, ALEXANDRA</creatorcontrib><creatorcontrib>FARROKH-SIAR, LILI</creatorcontrib><creatorcontrib>KOHEN, LEON</creatorcontrib><creatorcontrib>WIEDEMANN, PETER</creatorcontrib><creatorcontrib>HEIMANN, KLAUS</creatorcontrib><title>Iris Pigment Epithelial Cells of Long Evans Rats Demonstrate Phagocytic Activity</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The phagocytic activities of iris pigment epithelial (IPE) cells and retinal pigment epithelial (RPE) cells of Long Evans rats towards latex beads and rod outer segments (ROS) were compared in vitro. IPE and RPE cells of Long Evans rats were isolated and pure cultures obtained. The cultures were incubated with latex beads, fixed, and analysed computer morphometrically, IPE and RPE cell cultures were also incubated with isolated ROS and examined using transmission electron microscopy. IPE cells were able to ingest latex beads. There was no significant difference between the number of latex particles phagocytized by IPE and RPE cells. After incubation with isolated ROS, IPE cells also recognized and ingested the ROS particles. However, the specific phagocytic capacity of IPE cells was 76% of that of RPE cells.
The autologous IPE cells might have the potential to be used as an alternative to RPE cells for transplantation in the subretinal space.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured - cytology</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Iris - cytology</subject><subject>Iris - ultrastructure</subject><subject>iris pigment epithelium</subject><subject>latex</subject><subject>Long Evans rats</subject><subject>Microscopy, Electron</subject><subject>morphometric analysis</subject><subject>Phagocytosis</subject><subject>Pigment Epithelium of Eye - cytology</subject><subject>Rats</subject><subject>retinal pigment epithelium</subject><subject>Rod Cell Outer Segment</subject><subject>rod outer segments</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LAzEQhoMoWj-u3oQcxNvWpEk2m6PUqoWCRfQcstnZNrIfNUmL_femtPTmaWDmmXeGB6FbSoaUkPwRfsEPqVJySBiRJ2hAicozQog8RQNCKM94wcQFugzhO3UZl_wcnasRk0VOB2g-9S7guVu00EU8Wbm4hMaZBo-haQLuazzruwWebEwX8IeJAT9D23chehMBz5dm0dttdBY_2eg2Lm6v0VltmgA3h3qFvl4mn-O3bPb-Oh0_zTLLGY9ZbcSIicpyQiVjQGVlQRrGc5BC5LIoC0OKUgBVUEihcpEWSlWZkrO6pLlkV-hhn7vy_c8aQtStCzY9bTro10FLRVNQLhI43IPW9yF4qPXKu9b4raZE7xTqnUK9U6h3CtPC3SF5XbZQHfGDszS_P8xNsKapvemsC0dsVFA64iphxR6DZGHj0olgHXQWKufBRl317r8P_gCsFoxc</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>REZAI, KOUROUS A</creator><creator>LAPPAS, ALEXANDRA</creator><creator>FARROKH-SIAR, LILI</creator><creator>KOHEN, LEON</creator><creator>WIEDEMANN, PETER</creator><creator>HEIMANN, KLAUS</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970701</creationdate><title>Iris Pigment Epithelial Cells of Long Evans Rats Demonstrate Phagocytic Activity</title><author>REZAI, KOUROUS A ; LAPPAS, ALEXANDRA ; FARROKH-SIAR, LILI ; KOHEN, LEON ; WIEDEMANN, PETER ; HEIMANN, KLAUS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-fa5235dc401733e17dce7a346e755678b8a08b5e19e875965fa5b9dab43fb1673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured - cytology</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Iris - cytology</topic><topic>Iris - ultrastructure</topic><topic>iris pigment epithelium</topic><topic>latex</topic><topic>Long Evans rats</topic><topic>Microscopy, Electron</topic><topic>morphometric analysis</topic><topic>Phagocytosis</topic><topic>Pigment Epithelium of Eye - cytology</topic><topic>Rats</topic><topic>retinal pigment epithelium</topic><topic>Rod Cell Outer Segment</topic><topic>rod outer segments</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>REZAI, KOUROUS A</creatorcontrib><creatorcontrib>LAPPAS, ALEXANDRA</creatorcontrib><creatorcontrib>FARROKH-SIAR, LILI</creatorcontrib><creatorcontrib>KOHEN, LEON</creatorcontrib><creatorcontrib>WIEDEMANN, PETER</creatorcontrib><creatorcontrib>HEIMANN, KLAUS</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>REZAI, KOUROUS A</au><au>LAPPAS, ALEXANDRA</au><au>FARROKH-SIAR, LILI</au><au>KOHEN, LEON</au><au>WIEDEMANN, PETER</au><au>HEIMANN, KLAUS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Iris Pigment Epithelial Cells of Long Evans Rats Demonstrate Phagocytic Activity</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>65</volume><issue>1</issue><spage>23</spage><epage>29</epage><pages>23-29</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><coden>EXERA6</coden><abstract>The phagocytic activities of iris pigment epithelial (IPE) cells and retinal pigment epithelial (RPE) cells of Long Evans rats towards latex beads and rod outer segments (ROS) were compared in vitro. IPE and RPE cells of Long Evans rats were isolated and pure cultures obtained. The cultures were incubated with latex beads, fixed, and analysed computer morphometrically, IPE and RPE cell cultures were also incubated with isolated ROS and examined using transmission electron microscopy. IPE cells were able to ingest latex beads. There was no significant difference between the number of latex particles phagocytized by IPE and RPE cells. After incubation with isolated ROS, IPE cells also recognized and ingested the ROS particles. However, the specific phagocytic capacity of IPE cells was 76% of that of RPE cells.
The autologous IPE cells might have the potential to be used as an alternative to RPE cells for transplantation in the subretinal space.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>9237861</pmid><doi>10.1006/exer.1997.0307</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cells, Cultured - cytology Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Iris - cytology Iris - ultrastructure iris pigment epithelium latex Long Evans rats Microscopy, Electron morphometric analysis Phagocytosis Pigment Epithelium of Eye - cytology Rats retinal pigment epithelium Rod Cell Outer Segment rod outer segments Vertebrates: nervous system and sense organs |
| title | Iris Pigment Epithelial Cells of Long Evans Rats Demonstrate Phagocytic Activity |
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