Genetic and Immunologic Characterization of Viruses Infecting MN-rgp120-Vaccinated Volunteers
Proviral sequences were determined and immunologic characterization was carried out for envelope glycoproteins from 7 vaccinees who became infected with human immunodeficiency virus type 1 (HIV-1), through high-risk behavior, while participating in clinical trials of MN-rgp120, a candidate HIV-1 vac...
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| Veröffentlicht in: | The Journal of infectious diseases 1997-08, Vol.176 (2), p.384-397 |
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| container_title | The Journal of infectious diseases |
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| creator | Berman, Phillip W. Gray, Alane M. Wrin, Terri Vennari, Joann C. Eastman, Donna J. Nakamura, Gerald R. Francis, Donald P. Gorse, Geoffrey Schwartz, David H. |
| description | Proviral sequences were determined and immunologic characterization was carried out for envelope glycoproteins from 7 vaccinees who became infected with human immunodeficiency virus type 1 (HIV-1), through high-risk behavior, while participating in clinical trials of MN-rgp120, a candidate HIV-1 vaccine. All 7 infections resulted from subtype B viruses; however, only 3 of the viruses possessed the MN serotype-defining V3 domain sequence, IGPGRAF, prevalent in 60%–70% of US infections. Six of the 7 viruses differed from MN-rgp120 at a neutralizing epitope in the C4 domain, and all 7 differed from MN-rgp120 at a neutralizing epitope in the V2 domain. Recombinant gp120 was prepared from each breakthrough specimen and tested for binding to a panel of neutralizing monoclonal antibodies. The results suggest that 6 of 7 breakthrough infections may be related to incomplete immunization or to infection with viruses that differed from the vaccine immunogen at important virus-neutralizing epitopes. |
| doi_str_mv | 10.1086/514055 |
| format | Article |
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All 7 infections resulted from subtype B viruses; however, only 3 of the viruses possessed the MN serotype-defining V3 domain sequence, IGPGRAF, prevalent in 60%–70% of US infections. Six of the 7 viruses differed from MN-rgp120 at a neutralizing epitope in the C4 domain, and all 7 differed from MN-rgp120 at a neutralizing epitope in the V2 domain. Recombinant gp120 was prepared from each breakthrough specimen and tested for binding to a panel of neutralizing monoclonal antibodies. The results suggest that 6 of 7 breakthrough infections may be related to incomplete immunization or to infection with viruses that differed from the vaccine immunogen at important virus-neutralizing epitopes.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/514055</identifier><identifier>PMID: 9237703</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>AIDS Vaccines - genetics ; AIDS Vaccines - immunology ; AIDS/HIV ; Antibodies, Monoclonal ; Biological and medical sciences ; CD4-Positive T-Lymphocytes - virology ; Clinical Trials, Phase I as Topic ; Clinical Trials, Phase II as Topic ; Cloning, Molecular ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; HIV Antibodies - blood ; HIV Envelope Protein gp120 - genetics ; HIV Envelope Protein gp120 - immunology ; HIV Infections - immunology ; HIV Infections - virology ; HIV-1 - genetics ; HIV-1 - immunology ; Humans ; Male ; Microbiology ; Molecular Sequence Data ; Neutralization Tests ; Peptide Fragments - genetics ; Peptide Fragments - immunology ; Polymorphism, Genetic ; Recombinant Fusion Proteins ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, Synthetic - immunology ; Virology</subject><ispartof>The Journal of infectious diseases, 1997-08, Vol.176 (2), p.384-397</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Aug 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-223e69eeaaded2148349270b4abf506fa8a83169710b05fce766987b712849e13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2766359$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9237703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Berman, Phillip W.</creatorcontrib><creatorcontrib>Gray, Alane M.</creatorcontrib><creatorcontrib>Wrin, Terri</creatorcontrib><creatorcontrib>Vennari, Joann C.</creatorcontrib><creatorcontrib>Eastman, Donna J.</creatorcontrib><creatorcontrib>Nakamura, Gerald R.</creatorcontrib><creatorcontrib>Francis, Donald P.</creatorcontrib><creatorcontrib>Gorse, Geoffrey</creatorcontrib><creatorcontrib>Schwartz, David H.</creatorcontrib><title>Genetic and Immunologic Characterization of Viruses Infecting MN-rgp120-Vaccinated Volunteers</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Proviral sequences were determined and immunologic characterization was carried out for envelope glycoproteins from 7 vaccinees who became infected with human immunodeficiency virus type 1 (HIV-1), through high-risk behavior, while participating in clinical trials of MN-rgp120, a candidate HIV-1 vaccine. 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The results suggest that 6 of 7 breakthrough infections may be related to incomplete immunization or to infection with viruses that differed from the vaccine immunogen at important virus-neutralizing epitopes.</description><subject>AIDS Vaccines - genetics</subject><subject>AIDS Vaccines - immunology</subject><subject>AIDS/HIV</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Clinical Trials, Phase I as Topic</subject><subject>Clinical Trials, Phase II as Topic</subject><subject>Cloning, Molecular</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>HIV Antibodies - blood</subject><subject>HIV Envelope Protein gp120 - genetics</subject><subject>HIV Envelope Protein gp120 - immunology</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - virology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>Humans</subject><subject>Male</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Neutralization Tests</subject><subject>Peptide Fragments - genetics</subject><subject>Peptide Fragments - immunology</subject><subject>Polymorphism, Genetic</subject><subject>Recombinant Fusion Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Virology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoYzvqPxAKEXeleVSSylIap7thHpuxGQQJqdStNmNV0pOkYPTXm6GbXrhxdbicj8O5HITeEvyJ4FZ85qTBnD9DC8KZrIUg7DlaYExpTVqlXqJXKd1jjBsm5Bk6U5RJidkC_ViBh-xsZXxfbaZp9mEMu3Ivf5pobIbo_pjsgq_CUG1dnBOkauMHsNn5XXV1XcfdnlBcb421zpsMfbUN4-wzQEyv0YvBjAneHPUcfbv4ertc15c3q83yy2VtG0ZyTSkDoQCM6aGnpGlZo6jEXWO6gWMxmNa0jAglCe4wHyxIIVQrO0lo2ygg7Bx9POTuY3iYIWU9uWRhHI2HMCctFeGcUvVfkAjKBBaygO__Ae_DHH15Qpe2ijBW5JRmY0gpwqD30U0m_tYE66dV9GGVAr47ps3dBP0JO85Q_A9H3yRrxiEab106YbT8y_hT-_qAuZTh8WSb-EuXypLr9d13vZLL9d1Fe6sZ-wtDKKBI</recordid><startdate>19970801</startdate><enddate>19970801</enddate><creator>Berman, Phillip W.</creator><creator>Gray, Alane M.</creator><creator>Wrin, Terri</creator><creator>Vennari, Joann C.</creator><creator>Eastman, Donna J.</creator><creator>Nakamura, Gerald R.</creator><creator>Francis, Donald P.</creator><creator>Gorse, Geoffrey</creator><creator>Schwartz, David H.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970801</creationdate><title>Genetic and Immunologic Characterization of Viruses Infecting MN-rgp120-Vaccinated Volunteers</title><author>Berman, Phillip W. ; Gray, Alane M. ; Wrin, Terri ; Vennari, Joann C. ; Eastman, Donna J. ; Nakamura, Gerald R. ; Francis, Donald P. ; Gorse, Geoffrey ; Schwartz, David H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-223e69eeaaded2148349270b4abf506fa8a83169710b05fce766987b712849e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AIDS Vaccines - genetics</topic><topic>AIDS Vaccines - immunology</topic><topic>AIDS/HIV</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Clinical Trials, Phase I as Topic</topic><topic>Clinical Trials, Phase II as Topic</topic><topic>Cloning, Molecular</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>HIV Antibodies - blood</topic><topic>HIV Envelope Protein gp120 - genetics</topic><topic>HIV Envelope Protein gp120 - immunology</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - virology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>Humans</topic><topic>Male</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Neutralization Tests</topic><topic>Peptide Fragments - genetics</topic><topic>Peptide Fragments - immunology</topic><topic>Polymorphism, Genetic</topic><topic>Recombinant Fusion Proteins</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berman, Phillip W.</creatorcontrib><creatorcontrib>Gray, Alane M.</creatorcontrib><creatorcontrib>Wrin, Terri</creatorcontrib><creatorcontrib>Vennari, Joann C.</creatorcontrib><creatorcontrib>Eastman, Donna J.</creatorcontrib><creatorcontrib>Nakamura, Gerald R.</creatorcontrib><creatorcontrib>Francis, Donald P.</creatorcontrib><creatorcontrib>Gorse, Geoffrey</creatorcontrib><creatorcontrib>Schwartz, David H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Berman, Phillip W.</au><au>Gray, Alane M.</au><au>Wrin, Terri</au><au>Vennari, Joann C.</au><au>Eastman, Donna J.</au><au>Nakamura, Gerald R.</au><au>Francis, Donald P.</au><au>Gorse, Geoffrey</au><au>Schwartz, David H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and Immunologic Characterization of Viruses Infecting MN-rgp120-Vaccinated Volunteers</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1997-08-01</date><risdate>1997</risdate><volume>176</volume><issue>2</issue><spage>384</spage><epage>397</epage><pages>384-397</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Proviral sequences were determined and immunologic characterization was carried out for envelope glycoproteins from 7 vaccinees who became infected with human immunodeficiency virus type 1 (HIV-1), through high-risk behavior, while participating in clinical trials of MN-rgp120, a candidate HIV-1 vaccine. All 7 infections resulted from subtype B viruses; however, only 3 of the viruses possessed the MN serotype-defining V3 domain sequence, IGPGRAF, prevalent in 60%–70% of US infections. Six of the 7 viruses differed from MN-rgp120 at a neutralizing epitope in the C4 domain, and all 7 differed from MN-rgp120 at a neutralizing epitope in the V2 domain. Recombinant gp120 was prepared from each breakthrough specimen and tested for binding to a panel of neutralizing monoclonal antibodies. The results suggest that 6 of 7 breakthrough infections may be related to incomplete immunization or to infection with viruses that differed from the vaccine immunogen at important virus-neutralizing epitopes.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>9237703</pmid><doi>10.1086/514055</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 1997-08, Vol.176 (2), p.384-397 |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | AIDS Vaccines - genetics AIDS Vaccines - immunology AIDS/HIV Antibodies, Monoclonal Biological and medical sciences CD4-Positive T-Lymphocytes - virology Clinical Trials, Phase I as Topic Clinical Trials, Phase II as Topic Cloning, Molecular DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Genetic Variation HIV Antibodies - blood HIV Envelope Protein gp120 - genetics HIV Envelope Protein gp120 - immunology HIV Infections - immunology HIV Infections - virology HIV-1 - genetics HIV-1 - immunology Humans Male Microbiology Molecular Sequence Data Neutralization Tests Peptide Fragments - genetics Peptide Fragments - immunology Polymorphism, Genetic Recombinant Fusion Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - immunology Virology |
| title | Genetic and Immunologic Characterization of Viruses Infecting MN-rgp120-Vaccinated Volunteers |
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