Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy
The complete assignment of 1H and 13C chemical shifts for 99% uniformly 13C-labeled O-deacetylated glucuronoxylomannan (GXM) of Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determi...
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| Veröffentlicht in: | Carbohydrate research 1997-06, Vol.301 (1), p.33-40 |
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| creator | Sheng, Shuqun Cherniak, Robert |
| description | The complete assignment of
1H and
13C chemical shifts for 99% uniformly
13C-labeled
O-deacetylated glucuronoxylomannan (GXM) of
Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determined by a GHMBC experiment using 20% uniformly
13C-labeled GXM; GXM was prepared by a novel procedure that insured the virtual exclusion of adjacent
13C-labeled carbon atoms. For each residue in the GXM of 9759-Mu-1 we determined its linkage position, its anomeric configuration, and its position in the repeating sequence as follows:
▪
Using a
13C-labeled polysaccharide, the structure of GXM was determined by NMR spectroscopic methods to be |
| doi_str_mv | 10.1016/S0008-6215(97)00084-0 |
| format | Article |
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1H and
13C chemical shifts for 99% uniformly
13C-labeled
O-deacetylated glucuronoxylomannan (GXM) of
Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determined by a GHMBC experiment using 20% uniformly
13C-labeled GXM; GXM was prepared by a novel procedure that insured the virtual exclusion of adjacent
13C-labeled carbon atoms. For each residue in the GXM of 9759-Mu-1 we determined its linkage position, its anomeric configuration, and its position in the repeating sequence as follows:
▪
Using a
13C-labeled polysaccharide, the structure of GXM was determined by NMR spectroscopic methods to be</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>DOI: 10.1016/S0008-6215(97)00084-0</identifier><identifier>PMID: 9228737</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>13C Labeling, Structure ; 2D NMR spectroscopy ; Antigen ; Carbohydrate Conformation ; Carbohydrate Sequence ; Cryptococcus neoformans - chemistry ; Cryptococcus neoformans - classification ; GHMBC ; HCCH-COSY ; HCCH-TOCSY ; HSQC ; Magnetic Resonance Spectroscopy ; Molecular Sequence Data ; Monosaccharides - analysis ; Monosaccharides - chemistry ; Oligosaccharides - chemistry ; Polysaccharide ; Polysaccharides - chemistry ; Sequence Analysis ; Serotyping</subject><ispartof>Carbohydrate research, 1997-06, Vol.301 (1), p.33-40</ispartof><rights>1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2737-75441071084c0c9095cf024953d2b2e37c5e62f6177e6fa38ec377fa6ec0f74a3</citedby><cites>FETCH-LOGICAL-c2737-75441071084c0c9095cf024953d2b2e37c5e62f6177e6fa38ec377fa6ec0f74a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008621597000840$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9228737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sheng, Shuqun</creatorcontrib><creatorcontrib>Cherniak, Robert</creatorcontrib><title>Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>The complete assignment of
1H and
13C chemical shifts for 99% uniformly
13C-labeled
O-deacetylated glucuronoxylomannan (GXM) of
Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determined by a GHMBC experiment using 20% uniformly
13C-labeled GXM; GXM was prepared by a novel procedure that insured the virtual exclusion of adjacent
13C-labeled carbon atoms. For each residue in the GXM of 9759-Mu-1 we determined its linkage position, its anomeric configuration, and its position in the repeating sequence as follows:
▪
Using a
13C-labeled polysaccharide, the structure of GXM was determined by NMR spectroscopic methods to be</description><subject>13C Labeling, Structure</subject><subject>2D NMR spectroscopy</subject><subject>Antigen</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Cryptococcus neoformans - chemistry</subject><subject>Cryptococcus neoformans - classification</subject><subject>GHMBC</subject><subject>HCCH-COSY</subject><subject>HCCH-TOCSY</subject><subject>HSQC</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Sequence Data</subject><subject>Monosaccharides - analysis</subject><subject>Monosaccharides - chemistry</subject><subject>Oligosaccharides - chemistry</subject><subject>Polysaccharide</subject><subject>Polysaccharides - chemistry</subject><subject>Sequence Analysis</subject><subject>Serotyping</subject><issn>0008-6215</issn><issn>1873-426X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUMFO3DAUtBCIbhc-AcknRA8ptpPYm1O1WrUFaWEloFJvlvfluQQlcWo7VfMB_W-87IprT0-jmXlv3hBywdlnzri8fmSMLTIpeHlVqU87UGTsiMz4QuVZIeTPYzJ7l3wgH0N4SZBJJU_JaSVEkqkZ-fcY_Qhx9EidpfEZKc9XGfa-gWes6Sar0QDGqTUxwV_tCKN3vfs7ta4zfW_6nW3lpyE6cABjoD0663wiAw3oXZwGpEtaY0TfNX1asp3o_d0DDQNC9C6AG6YzcmJNG_D8MOfkx7evT6ubbL35frtarjMQKW2myqLgTPH0KjCoWFWCZaKoyrwWW4G5ghKlsJIrhdKafIGQK2WNRGBWFSafk8v93sG73yOGqLsmALatSanHoFXFi1KpMgnLvRBSwuDR6sE3nfGT5kzv6tdv9etdt7pS-q1-zZLv4nBg3HZYv7sOfSf-y57H9OWfBr0O0GAPWDc-1aFr1_znwisz15ay</recordid><startdate>19970611</startdate><enddate>19970611</enddate><creator>Sheng, Shuqun</creator><creator>Cherniak, Robert</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970611</creationdate><title>Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy</title><author>Sheng, Shuqun ; Cherniak, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2737-75441071084c0c9095cf024953d2b2e37c5e62f6177e6fa38ec377fa6ec0f74a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>13C Labeling, Structure</topic><topic>2D NMR spectroscopy</topic><topic>Antigen</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Cryptococcus neoformans - chemistry</topic><topic>Cryptococcus neoformans - classification</topic><topic>GHMBC</topic><topic>HCCH-COSY</topic><topic>HCCH-TOCSY</topic><topic>HSQC</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Sequence Data</topic><topic>Monosaccharides - analysis</topic><topic>Monosaccharides - chemistry</topic><topic>Oligosaccharides - chemistry</topic><topic>Polysaccharide</topic><topic>Polysaccharides - chemistry</topic><topic>Sequence Analysis</topic><topic>Serotyping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sheng, Shuqun</creatorcontrib><creatorcontrib>Cherniak, Robert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sheng, Shuqun</au><au>Cherniak, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>1997-06-11</date><risdate>1997</risdate><volume>301</volume><issue>1</issue><spage>33</spage><epage>40</epage><pages>33-40</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><abstract>The complete assignment of
1H and
13C chemical shifts for 99% uniformly
13C-labeled
O-deacetylated glucuronoxylomannan (GXM) of
Cryptococcus neoformans serotype A isolate 9759-Mu-1 was accomplished by the analysis of HCCH-TOCSY and HCCH-COSY spectra. The sequence of the glycosyl residues was determined by a GHMBC experiment using 20% uniformly
13C-labeled GXM; GXM was prepared by a novel procedure that insured the virtual exclusion of adjacent
13C-labeled carbon atoms. For each residue in the GXM of 9759-Mu-1 we determined its linkage position, its anomeric configuration, and its position in the repeating sequence as follows:
▪
Using a
13C-labeled polysaccharide, the structure of GXM was determined by NMR spectroscopic methods to be</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>9228737</pmid><doi>10.1016/S0008-6215(97)00084-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Carbohydrate research, 1997-06, Vol.301 (1), p.33-40 |
| issn | 0008-6215 1873-426X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 13C Labeling, Structure 2D NMR spectroscopy Antigen Carbohydrate Conformation Carbohydrate Sequence Cryptococcus neoformans - chemistry Cryptococcus neoformans - classification GHMBC HCCH-COSY HCCH-TOCSY HSQC Magnetic Resonance Spectroscopy Molecular Sequence Data Monosaccharides - analysis Monosaccharides - chemistry Oligosaccharides - chemistry Polysaccharide Polysaccharides - chemistry Sequence Analysis Serotyping |
| title | Structure of the 13C-enriched O-deacetylated glucuronoxylomannan of Cryptococcus neoformans serotype A determined by NMR spectroscopy |
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