Optimization of the Chiral Inversion of 2-Phenylpropionic Acid by Verticillium lecanii
Previous studies have demonstrated that Verticillium lecanii might be used as a microbial model of the inversion of 2‐arylpropionic acids in man. This paper describes the optimization of the inversion process in respect of culture medium, pH, cell density and substrate concentration. The study demon...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 1997-03, Vol.49 (3), p.263-269 |
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| container_title | Journal of pharmacy and pharmacology |
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| creator | THOMASON, MICHAEL J. RHYS-WILLIAMS, WILLIAM LLOYD, ANDREW W. HANLON, GEOFFREY W. |
| description | Previous studies have demonstrated that Verticillium lecanii might be used as a microbial model of the inversion of 2‐arylpropionic acids in man. This paper describes the optimization of the inversion process in respect of culture medium, pH, cell density and substrate concentration.
The study demonstrates that optimum inversion occurs in Sørensen's phosphate buffer at pH 5.5. The extent and rate of inversion were also shown to be dependent on substrate concentration and cell density.
This study will form the basis of the development of a microbial model of the metabolism of 2‐arylpropionic acids which might be suitable for the in‐vitro screening of new compounds in this class. |
| doi_str_mv | 10.1111/j.2042-7158.1997.tb06792.x |
| format | Article |
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The study demonstrates that optimum inversion occurs in Sørensen's phosphate buffer at pH 5.5. The extent and rate of inversion were also shown to be dependent on substrate concentration and cell density.
This study will form the basis of the development of a microbial model of the metabolism of 2‐arylpropionic acids which might be suitable for the in‐vitro screening of new compounds in this class.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/j.2042-7158.1997.tb06792.x</identifier><identifier>PMID: 9231342</identifier><identifier>CODEN: JPPMAB</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Bones, joints and connective tissue. Antiinflammatory agents ; Buffers ; Culture Media ; Hydrogen-Ion Concentration ; Medical sciences ; Mitosporic Fungi - growth & development ; Mitosporic Fungi - metabolism ; Pharmacology. Drug treatments ; Phenylpropionates - chemistry ; Phenylpropionates - metabolism ; Stereoisomerism ; Verticillium lecanii</subject><ispartof>Journal of pharmacy and pharmacology, 1997-03, Vol.49 (3), p.263-269</ispartof><rights>1997 Royal Pharmaceutical Society of Great Britain</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5212-89c02e50310eba3d727553d1a2c0520cf0236d51c5305b1ce3d3bf997420faa53</citedby><cites>FETCH-LOGICAL-c5212-89c02e50310eba3d727553d1a2c0520cf0236d51c5305b1ce3d3bf997420faa53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.2042-7158.1997.tb06792.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.2042-7158.1997.tb06792.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2625634$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9231342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>THOMASON, MICHAEL J.</creatorcontrib><creatorcontrib>RHYS-WILLIAMS, WILLIAM</creatorcontrib><creatorcontrib>LLOYD, ANDREW W.</creatorcontrib><creatorcontrib>HANLON, GEOFFREY W.</creatorcontrib><title>Optimization of the Chiral Inversion of 2-Phenylpropionic Acid by Verticillium lecanii</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Previous studies have demonstrated that Verticillium lecanii might be used as a microbial model of the inversion of 2‐arylpropionic acids in man. This paper describes the optimization of the inversion process in respect of culture medium, pH, cell density and substrate concentration.
The study demonstrates that optimum inversion occurs in Sørensen's phosphate buffer at pH 5.5. The extent and rate of inversion were also shown to be dependent on substrate concentration and cell density.
This study will form the basis of the development of a microbial model of the metabolism of 2‐arylpropionic acids which might be suitable for the in‐vitro screening of new compounds in this class.</description><subject>Biological and medical sciences</subject><subject>Bones, joints and connective tissue. Antiinflammatory agents</subject><subject>Buffers</subject><subject>Culture Media</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Mitosporic Fungi - growth & development</subject><subject>Mitosporic Fungi - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenylpropionates - chemistry</subject><subject>Phenylpropionates - metabolism</subject><subject>Stereoisomerism</subject><subject>Verticillium lecanii</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEGP0zAQhS0EWsrCT0CKEOKWMB7HdsKJVQXbRSu2ByjcLMdxVBcnKXYKLb-eRI16Rfhia96bN-OPkFcUMjqet7sMIcdUUl5ktCxlNlQgZInZ8RFZXKTHZAGAmDIu2VPyLMYdAEghxBW5KpFRluOCbB72g2vdHz24vkv6Jhm2NlluXdA-uet-2RDnOqbrre1Ofh_6_VhyJrkxrk6qU7KxYXDGee8ObeKt0Z1zz8mTRvtoX8z3Nfn68cOX5Sq9f7i9W97cp4YjxbQoDaDlwCjYSrNaouSc1VSjAY5gGkAmak4NZ8AraiyrWdWMP84RGq05uyZvzrnjWj8PNg6qddFY73Vn-0NUsqQ5lxT-aaQCCsbFlPjubDShjzHYRu2Da3U4KQpqoq92akKsJsRqoq9m-uo4Nr-cpxyq1taX1hn3qL-edR2N9k3QnXHxYkOBXLB8tL0_2347b0__sYD6tF6tp-cYkZ4jXBzs8RKhww8lJJNcfft8qzhdb_LvxUoV7C_xU7Af</recordid><startdate>199703</startdate><enddate>199703</enddate><creator>THOMASON, MICHAEL J.</creator><creator>RHYS-WILLIAMS, WILLIAM</creator><creator>LLOYD, ANDREW W.</creator><creator>HANLON, GEOFFREY W.</creator><general>Blackwell Publishing Ltd</general><general>Pharmaceutical Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199703</creationdate><title>Optimization of the Chiral Inversion of 2-Phenylpropionic Acid by Verticillium lecanii</title><author>THOMASON, MICHAEL J. ; RHYS-WILLIAMS, WILLIAM ; LLOYD, ANDREW W. ; HANLON, GEOFFREY W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5212-89c02e50310eba3d727553d1a2c0520cf0236d51c5305b1ce3d3bf997420faa53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Bones, joints and connective tissue. Antiinflammatory agents</topic><topic>Buffers</topic><topic>Culture Media</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Mitosporic Fungi - growth & development</topic><topic>Mitosporic Fungi - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenylpropionates - chemistry</topic><topic>Phenylpropionates - metabolism</topic><topic>Stereoisomerism</topic><topic>Verticillium lecanii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THOMASON, MICHAEL J.</creatorcontrib><creatorcontrib>RHYS-WILLIAMS, WILLIAM</creatorcontrib><creatorcontrib>LLOYD, ANDREW W.</creatorcontrib><creatorcontrib>HANLON, GEOFFREY W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>THOMASON, MICHAEL J.</au><au>RHYS-WILLIAMS, WILLIAM</au><au>LLOYD, ANDREW W.</au><au>HANLON, GEOFFREY W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of the Chiral Inversion of 2-Phenylpropionic Acid by Verticillium lecanii</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>1997-03</date><risdate>1997</risdate><volume>49</volume><issue>3</issue><spage>263</spage><epage>269</epage><pages>263-269</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><coden>JPPMAB</coden><abstract>Previous studies have demonstrated that Verticillium lecanii might be used as a microbial model of the inversion of 2‐arylpropionic acids in man. This paper describes the optimization of the inversion process in respect of culture medium, pH, cell density and substrate concentration.
The study demonstrates that optimum inversion occurs in Sørensen's phosphate buffer at pH 5.5. The extent and rate of inversion were also shown to be dependent on substrate concentration and cell density.
This study will form the basis of the development of a microbial model of the metabolism of 2‐arylpropionic acids which might be suitable for the in‐vitro screening of new compounds in this class.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9231342</pmid><doi>10.1111/j.2042-7158.1997.tb06792.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of pharmacy and pharmacology, 1997-03, Vol.49 (3), p.263-269 |
| issn | 0022-3573 2042-7158 |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological and medical sciences Bones, joints and connective tissue. Antiinflammatory agents Buffers Culture Media Hydrogen-Ion Concentration Medical sciences Mitosporic Fungi - growth & development Mitosporic Fungi - metabolism Pharmacology. Drug treatments Phenylpropionates - chemistry Phenylpropionates - metabolism Stereoisomerism Verticillium lecanii |
| title | Optimization of the Chiral Inversion of 2-Phenylpropionic Acid by Verticillium lecanii |
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