Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. [Erratum: Sept 15, 1997, v. 154 (2), p. 423-424.]

A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a lambda gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial...

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Veröffentlicht in:	FEMS microbiology letters 1997-07, Vol.152 (1), p.183-188
Hauptverfasser:	Arai, T, Yusa, S, Kirimura, K, Usami, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Base Sequence cDNA Cloning Cloning, Molecular DNA, Complementary - chemistry genbank/ab000260 Hybridization Lipase Lipase - chemistry Lipase - genetics Microbiology Molecular Sequence Data Oligonucleotides sequence alignment Trichosporon - enzymology Trichosporon - genetics Trichosporon fermentans Trimers
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