Improved methods for in situ enzymatic amplification and detection of low copy number genes in bacteria

Abstract We present alternative and improved protocols for in situ analysis of single copy genes in prokaryotes. Primed in situ amplification (PRINS) and cycle PRINS were used to detect, via the incorporation of a fluorescein labelled nucleotide, the presence of specific genes carried on both high a...

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Veröffentlicht in:	FEMS microbiology letters 1997-07, Vol.152 (1), p.65-73
Hauptverfasser:	Jacobs, Daniel, Angles, Mark L, Goodman, Amanda E, Neilan, Brett A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Bacteria Coliforms Copy number E coli Escherichia coli Fluorescein Fluorescence Gene Gene Dosage Genes Genes, Bacterial In situ Microbiology Microorganisms Nucleic acids Nucleotides Plasmid Plasmids Polymerase Chain Reaction Primed in situ amplification Prokaryotes
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