Plasmid instability kinetics in continuous culture of a recombinant Saccharomyces cerevisiae in airlift bioreactor

Plasmid instability of a recombinant Saccharomyces cerevisiae C468/pGAC9 (ATCC 20690) was examined during continuous culture in a nonselective medium in an airlift bioreactor. The recombinant strain contained a 2- μm based shuttle vector pGAC9 and expresses Aspergillus awamori glucoamylase gene unde...

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Veröffentlicht in:	Journal of biotechnology 1997-05, Vol.55 (1), p.31-41
Hauptverfasser:	Zhang, Zhigen, Scharer, Jeno M, Moo-Young, Murray
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biological Sciences Bioreactors Biotechnology Dilution rate Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism Gene Dosage Gene Expression Regulation, Fungal Genetic engineering Genetic technics Glucan 1,4-alpha-Glucosidase - biosynthesis Glucan 1,4-alpha-Glucosidase - genetics Glucoamylase Kinetics Methods. Procedures. Technologies Models, Genetic plant breeding plant genetics Plasmid stability Plasmids Recombinant Proteins - biosynthesis Recombinant yeast Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Vectors (cloning, transfer, expression). Insertion sequences and transposons
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