Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257
Summary Trimethoprim resistance mediated by the Staphylococcus aureus multi‐resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn4003. Nucleotide sequence analysis of Tn4003 revealed it to be 4717 bp in length and to contain thre...
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| Veröffentlicht in: | Molecular microbiology 1989, Vol.3 (2), p.161-175 |
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| creator | Rouch, D. A. Messerotti, L. J. Loo, L. S. L. Jackson, C. A. Skurray, R. A. |
| description | Summary
Trimethoprim resistance mediated by the Staphylococcus aureus multi‐resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn4003. Nucleotide sequence analysis of Tn4003 revealed it to be 4717 bp in length and to contain three copies of the insertion element IS257 (789‐790 bp), the outside two of which are flanked by directly repeated 8‐bp target sequences. IS257 has imperfect terminal inverted repeats of 27‐28 bp and encodes for a putative transposase with two potential α‐helix‐turn‐α‐helix DNA recognition motifs. IS257 shares sequence similarities with members of the IS15 family of insertion sequences from Gram‐negative bacteria and with ISS 1 from Streptococcus lactis. The central region of the transposon contains the dfrA gene that specifies the S1 dihydrofolate reductase (DHFR) responsible for trimethoprim resistance. The S1 enzyme shows sequence homology with type I and V trimethoprim‐resistant DHFRs from Gram‐negative bacteria and with chromosomally encoded DHFRs from Gram‐positive and Gram‐negative bacteria. 5’to dfrA is a thymidylate synthetase gene, designated thyE. |
| doi_str_mv | 10.1111/j.1365-2958.1989.tb01805.x |
| format | Article |
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Trimethoprim resistance mediated by the Staphylococcus aureus multi‐resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn4003. Nucleotide sequence analysis of Tn4003 revealed it to be 4717 bp in length and to contain three copies of the insertion element IS257 (789‐790 bp), the outside two of which are flanked by directly repeated 8‐bp target sequences. IS257 has imperfect terminal inverted repeats of 27‐28 bp and encodes for a putative transposase with two potential α‐helix‐turn‐α‐helix DNA recognition motifs. IS257 shares sequence similarities with members of the IS15 family of insertion sequences from Gram‐negative bacteria and with ISS 1 from Streptococcus lactis. The central region of the transposon contains the dfrA gene that specifies the S1 dihydrofolate reductase (DHFR) responsible for trimethoprim resistance. The S1 enzyme shows sequence homology with type I and V trimethoprim‐resistant DHFRs from Gram‐negative bacteria and with chromosomally encoded DHFRs from Gram‐positive and Gram‐negative bacteria. 5’to dfrA is a thymidylate synthetase gene, designated thyE.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1989.tb01805.x</identifier><identifier>PMID: 2548057</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biological Evolution ; DNA Transposable Elements ; DNA, Bacterial ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; Gram-Negative Bacteria - genetics ; Microbiology ; Molecular Sequence Data ; Nucleotidyltransferases - genetics ; R Factors - genetics ; Repetitive Sequences, Nucleic Acid ; Restriction Mapping ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - enzymology ; Staphylococcus aureus - genetics ; Tetrahydrofolate Dehydrogenase - genetics ; Thymidylate Synthase - genetics ; Transposases ; Trimethoprim Resistance - genetics</subject><ispartof>Molecular microbiology, 1989, Vol.3 (2), p.161-175</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1989.tb01805.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1989.tb01805.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,4025,27927,27928,27929,45578,45579</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7272145$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2548057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rouch, D. A.</creatorcontrib><creatorcontrib>Messerotti, L. J.</creatorcontrib><creatorcontrib>Loo, L. S. L.</creatorcontrib><creatorcontrib>Jackson, C. A.</creatorcontrib><creatorcontrib>Skurray, R. A.</creatorcontrib><title>Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Trimethoprim resistance mediated by the Staphylococcus aureus multi‐resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn4003. Nucleotide sequence analysis of Tn4003 revealed it to be 4717 bp in length and to contain three copies of the insertion element IS257 (789‐790 bp), the outside two of which are flanked by directly repeated 8‐bp target sequences. IS257 has imperfect terminal inverted repeats of 27‐28 bp and encodes for a putative transposase with two potential α‐helix‐turn‐α‐helix DNA recognition motifs. IS257 shares sequence similarities with members of the IS15 family of insertion sequences from Gram‐negative bacteria and with ISS 1 from Streptococcus lactis. The central region of the transposon contains the dfrA gene that specifies the S1 dihydrofolate reductase (DHFR) responsible for trimethoprim resistance. The S1 enzyme shows sequence homology with type I and V trimethoprim‐resistant DHFRs from Gram‐negative bacteria and with chromosomally encoded DHFRs from Gram‐positive and Gram‐negative bacteria. 5’to dfrA is a thymidylate synthetase gene, designated thyE.</description><subject>Amino Acid Sequence</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleotidyltransferases - genetics</subject><subject>R Factors - genetics</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Restriction Mapping</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - enzymology</subject><subject>Staphylococcus aureus - genetics</subject><subject>Tetrahydrofolate Dehydrogenase - genetics</subject><subject>Thymidylate Synthase - genetics</subject><subject>Transposases</subject><subject>Trimethoprim Resistance - genetics</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQxiMEKkvhEZAshLgl-G8SX5BQBWWlVhy6SNwsxx6TLEkc7EQ078RD1mlXe8WH-Q7fT59HM5Nl7wguSHofjwVhpcipFHVBZC2LucGkxqK4f5btztbzbIelwDmr6c-X2asYjxgThkt2kV1QwRNf7bJ_h9ANMLd-SooCxC7OejSA5qDHOPnoR3QYOcYMueAHdDfrqV17b7wxS0R6CZAERuMtRPQLxlSdD0gj27WrDd75Xs-Qku1iZh0B6dGiuV2Hzq6PTlzHuYVHy_V6_A0WNWsiAgAyfupSoHdof0dF9Tp74XQf4c1JL7MfX78crr7lN9-v91efb_IjY4TkkpXALKFOWldKTXWjCdfWaYIbY0gjDKe4ohUwI5qGcSmINawSlXQcKK7ZZfbhKXcK_s8CcVZDFw30qT3wS1SVJBzLWvwXJIJVnHKSwLcncGkGsGqbtg6rOu0h-e9Pvo5G9y4N33TxjKVmKeHbf5-esL9dD-vZJlhtd6GOalu-2pavtrtQp7tQ9-r2dk9Kwh4AW3ywAQ</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Rouch, D. A.</creator><creator>Messerotti, L. J.</creator><creator>Loo, L. S. L.</creator><creator>Jackson, C. A.</creator><creator>Skurray, R. A.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257</title><author>Rouch, D. A. ; Messerotti, L. J. ; Loo, L. S. L. ; Jackson, C. A. ; Skurray, R. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3311-936e3d12f9df69a2aba14adfa10bcc1b5c420727e3c5bb34951dc37579f4e2083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleotidyltransferases - genetics</topic><topic>R Factors - genetics</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Restriction Mapping</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - enzymology</topic><topic>Staphylococcus aureus - genetics</topic><topic>Tetrahydrofolate Dehydrogenase - genetics</topic><topic>Thymidylate Synthase - genetics</topic><topic>Transposases</topic><topic>Trimethoprim Resistance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rouch, D. A.</creatorcontrib><creatorcontrib>Messerotti, L. J.</creatorcontrib><creatorcontrib>Loo, L. S. L.</creatorcontrib><creatorcontrib>Jackson, C. A.</creatorcontrib><creatorcontrib>Skurray, R. A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rouch, D. A.</au><au>Messerotti, L. J.</au><au>Loo, L. S. L.</au><au>Jackson, C. A.</au><au>Skurray, R. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1989</date><risdate>1989</risdate><volume>3</volume><issue>2</issue><spage>161</spage><epage>175</epage><pages>161-175</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Trimethoprim resistance mediated by the Staphylococcus aureus multi‐resistance plasmid pSK1 is encoded by a structure with characteristics of a composite transposon which we have designated Tn4003. Nucleotide sequence analysis of Tn4003 revealed it to be 4717 bp in length and to contain three copies of the insertion element IS257 (789‐790 bp), the outside two of which are flanked by directly repeated 8‐bp target sequences. IS257 has imperfect terminal inverted repeats of 27‐28 bp and encodes for a putative transposase with two potential α‐helix‐turn‐α‐helix DNA recognition motifs. IS257 shares sequence similarities with members of the IS15 family of insertion sequences from Gram‐negative bacteria and with ISS 1 from Streptococcus lactis. The central region of the transposon contains the dfrA gene that specifies the S1 dihydrofolate reductase (DHFR) responsible for trimethoprim resistance. The S1 enzyme shows sequence homology with type I and V trimethoprim‐resistant DHFRs from Gram‐negative bacteria and with chromosomally encoded DHFRs from Gram‐positive and Gram‐negative bacteria. 5’to dfrA is a thymidylate synthetase gene, designated thyE.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2548057</pmid><doi>10.1111/j.1365-2958.1989.tb01805.x</doi><tpages>15</tpages></addata></record> |
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| ispartof | Molecular microbiology, 1989, Vol.3 (2), p.161-175 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Biological Evolution DNA Transposable Elements DNA, Bacterial Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Gram-Negative Bacteria - genetics Microbiology Molecular Sequence Data Nucleotidyltransferases - genetics R Factors - genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Staphylococcus aureus - drug effects Staphylococcus aureus - enzymology Staphylococcus aureus - genetics Tetrahydrofolate Dehydrogenase - genetics Thymidylate Synthase - genetics Transposases Trimethoprim Resistance - genetics |
| title | Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257 |
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