Normal pattern of labeling of cerebral cortical corticotropin-releasing factor (CRF) receptors in alzheimer's disease: Evidence from chemical cross-linking studies
Recent data have demonstrated that in Alzheimer's disease, the concentrations of corticotropin-releasing factor (CRF) were reduced and that there were reciprocal increases in CRF receptors in affected cerebrocortical areas. In order to determine whether the increases in CRF receptors in Alzheim...
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| Veröffentlicht in: | Neuropharmacology 1989-07, Vol.28 (7), p.761-764 |
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| container_title | Neuropharmacology |
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| creator | Grigoriadis, D.E. Struble, B.G. Prica, D.L. De Sauza, B.B. |
| description | Recent data have demonstrated that in Alzheimer's disease, the concentrations of corticotropin-releasing factor (CRF) were reduced and that there were reciprocal increases in CRF receptors in affected cerebrocortical areas. In order to determine whether the increases in CRF receptors in Alzheimer's disease were due to altered molecular composition of the binding protein, we compared the labeling pattern of
125I-Tyr
0ovine CRF in temporal neocortex of Alzheimer's patients and age-matched controls using chemical cross-linking techniques. A similar pattern of
125I-Tyr
0ovine CBF labeling was seen in Alzheimer's and control brains, with the major CRF binding protein corresponding to an apparent molecular weight of 58,000 Da. These data indicate that the increased CRF receptor population in cerebral cortex in Alzheimer's disease comprises bona fide CRF receptor binding subunits with no apparent change in the molecular structure. |
| doi_str_mv | 10.1016/0028-3908(89)90164-0 |
| format | Article |
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125I-Tyr
0ovine CRF in temporal neocortex of Alzheimer's patients and age-matched controls using chemical cross-linking techniques. A similar pattern of
125I-Tyr
0ovine CBF labeling was seen in Alzheimer's and control brains, with the major CRF binding protein corresponding to an apparent molecular weight of 58,000 Da. These data indicate that the increased CRF receptor population in cerebral cortex in Alzheimer's disease comprises bona fide CRF receptor binding subunits with no apparent change in the molecular structure.</description><identifier>ISSN: 0028-3908</identifier><identifier>EISSN: 1873-7064</identifier><identifier>DOI: 10.1016/0028-3908(89)90164-0</identifier><identifier>PMID: 2548115</identifier><identifier>CODEN: NEPHBW</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>affinity cross-linking ; Aged ; Aged, 80 and over ; Alzheimer Disease - metabolism ; Alzheimer's Disease ; Autoradiography ; Biological and medical sciences ; Brain Chemistry - drug effects ; Cerebral Cortex - metabolism ; Choline O-Acetyltransferase - metabolism ; Corticotropin-Releasing Factor (CRF) ; Cross-Linking Reagents ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Female ; Humans ; Iodine Radioisotopes ; Male ; Medical sciences ; Membrane Proteins - metabolism ; Middle Aged ; Molecular Weight ; Neurology ; Receptors ; Receptors, Corticotropin-Releasing Hormone ; Receptors, Neurotransmitter - metabolism</subject><ispartof>Neuropharmacology, 1989-07, Vol.28 (7), p.761-764</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-3baa79020a8c469d15e04c8485d74f9726b5cf380f0fbb5e606d743c8cbd09f93</citedby><cites>FETCH-LOGICAL-c386t-3baa79020a8c469d15e04c8485d74f9726b5cf380f0fbb5e606d743c8cbd09f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0028-3908(89)90164-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6769150$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2548115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grigoriadis, D.E.</creatorcontrib><creatorcontrib>Struble, B.G.</creatorcontrib><creatorcontrib>Prica, D.L.</creatorcontrib><creatorcontrib>De Sauza, B.B.</creatorcontrib><title>Normal pattern of labeling of cerebral cortical corticotropin-releasing factor (CRF) receptors in alzheimer's disease: Evidence from chemical cross-linking studies</title><title>Neuropharmacology</title><addtitle>Neuropharmacology</addtitle><description>Recent data have demonstrated that in Alzheimer's disease, the concentrations of corticotropin-releasing factor (CRF) were reduced and that there were reciprocal increases in CRF receptors in affected cerebrocortical areas. In order to determine whether the increases in CRF receptors in Alzheimer's disease were due to altered molecular composition of the binding protein, we compared the labeling pattern of
125I-Tyr
0ovine CRF in temporal neocortex of Alzheimer's patients and age-matched controls using chemical cross-linking techniques. A similar pattern of
125I-Tyr
0ovine CBF labeling was seen in Alzheimer's and control brains, with the major CRF binding protein corresponding to an apparent molecular weight of 58,000 Da. These data indicate that the increased CRF receptor population in cerebral cortex in Alzheimer's disease comprises bona fide CRF receptor binding subunits with no apparent change in the molecular structure.</description><subject>affinity cross-linking</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alzheimer Disease - metabolism</subject><subject>Alzheimer's Disease</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Brain Chemistry - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Choline O-Acetyltransferase - metabolism</subject><subject>Corticotropin-Releasing Factor (CRF)</subject><subject>Cross-Linking Reagents</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Female</subject><subject>Humans</subject><subject>Iodine Radioisotopes</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Proteins - metabolism</subject><subject>Middle Aged</subject><subject>Molecular Weight</subject><subject>Neurology</subject><subject>Receptors</subject><subject>Receptors, Corticotropin-Releasing Hormone</subject><subject>Receptors, Neurotransmitter - metabolism</subject><issn>0028-3908</issn><issn>1873-7064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFu1DAQtRBVWRb-ACQfUGkPgfEmcWwOldCqpUgVSAjOlmOPqSGJg-2tBL_Dj-J0V3vk5Od5b96M3hDygsEbBoy_BdiIqpYgzoW8kKXSVPCIrJjo6qoD3jwmq6PkCXma0g8AaAQTp-R00xbA2hX5-ynEUQ901jljnGhwdNA9Dn76vmCDEftYeBNi9uYIQo5h9lMVcUCdFrHTJodIz7dfri9oRINz-SbqJ6qHP3foR4yvE7U-FT2-o1f33uJkkLoYRmrucNy7x5BSVab_XDxT3lmP6Rk5cXpI-Pzwrsm366uv25vq9vOHj9v3t5WpBc9V3WvdSdiAFqbh0rIWoTGiEa3tGie7De9b42oBDlzft8iBF6I2wvQWpJP1mpztfecYfu0wZTX6ZHAY9IRhl1QnWRlUWtak2Qsf1o3o1Bz9qONvxUAtt1FL8GoJXgmpHm6joLS9PPjv-hHtselwjMK_OvA6lTBc1JPx6SjjHZesXWwu9zIsWdx7jCoZv2Rpfck9Kxv8__f4B1T4rcM</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Grigoriadis, D.E.</creator><creator>Struble, B.G.</creator><creator>Prica, D.L.</creator><creator>De Sauza, B.B.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890701</creationdate><title>Normal pattern of labeling of cerebral cortical corticotropin-releasing factor (CRF) receptors in alzheimer's disease: Evidence from chemical cross-linking studies</title><author>Grigoriadis, D.E. ; Struble, B.G. ; Prica, D.L. ; De Sauza, B.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-3baa79020a8c469d15e04c8485d74f9726b5cf380f0fbb5e606d743c8cbd09f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>affinity cross-linking</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alzheimer Disease - metabolism</topic><topic>Alzheimer's Disease</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Brain Chemistry - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Choline O-Acetyltransferase - metabolism</topic><topic>Corticotropin-Releasing Factor (CRF)</topic><topic>Cross-Linking Reagents</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Female</topic><topic>Humans</topic><topic>Iodine Radioisotopes</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Proteins - metabolism</topic><topic>Middle Aged</topic><topic>Molecular Weight</topic><topic>Neurology</topic><topic>Receptors</topic><topic>Receptors, Corticotropin-Releasing Hormone</topic><topic>Receptors, Neurotransmitter - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grigoriadis, D.E.</creatorcontrib><creatorcontrib>Struble, B.G.</creatorcontrib><creatorcontrib>Prica, D.L.</creatorcontrib><creatorcontrib>De Sauza, B.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuropharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grigoriadis, D.E.</au><au>Struble, B.G.</au><au>Prica, D.L.</au><au>De Sauza, B.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Normal pattern of labeling of cerebral cortical corticotropin-releasing factor (CRF) receptors in alzheimer's disease: Evidence from chemical cross-linking studies</atitle><jtitle>Neuropharmacology</jtitle><addtitle>Neuropharmacology</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>28</volume><issue>7</issue><spage>761</spage><epage>764</epage><pages>761-764</pages><issn>0028-3908</issn><eissn>1873-7064</eissn><coden>NEPHBW</coden><abstract>Recent data have demonstrated that in Alzheimer's disease, the concentrations of corticotropin-releasing factor (CRF) were reduced and that there were reciprocal increases in CRF receptors in affected cerebrocortical areas. In order to determine whether the increases in CRF receptors in Alzheimer's disease were due to altered molecular composition of the binding protein, we compared the labeling pattern of
125I-Tyr
0ovine CRF in temporal neocortex of Alzheimer's patients and age-matched controls using chemical cross-linking techniques. A similar pattern of
125I-Tyr
0ovine CBF labeling was seen in Alzheimer's and control brains, with the major CRF binding protein corresponding to an apparent molecular weight of 58,000 Da. These data indicate that the increased CRF receptor population in cerebral cortex in Alzheimer's disease comprises bona fide CRF receptor binding subunits with no apparent change in the molecular structure.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2548115</pmid><doi>10.1016/0028-3908(89)90164-0</doi><tpages>4</tpages></addata></record> |
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| subjects | affinity cross-linking Aged Aged, 80 and over Alzheimer Disease - metabolism Alzheimer's Disease Autoradiography Biological and medical sciences Brain Chemistry - drug effects Cerebral Cortex - metabolism Choline O-Acetyltransferase - metabolism Corticotropin-Releasing Factor (CRF) Cross-Linking Reagents Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Female Humans Iodine Radioisotopes Male Medical sciences Membrane Proteins - metabolism Middle Aged Molecular Weight Neurology Receptors Receptors, Corticotropin-Releasing Hormone Receptors, Neurotransmitter - metabolism |
| title | Normal pattern of labeling of cerebral cortical corticotropin-releasing factor (CRF) receptors in alzheimer's disease: Evidence from chemical cross-linking studies |
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