Characterization of human liver inducible nitric oxide synthase expressed in Escherichia coli

We have cloned the human liver inducible isoform of nitric oxide synthase (NOS) into an Escherichia coli expression vector and have expressed and purified the enzyme. The protein has been expressed with and without a polyhistidine tail. In both cases, expression of functional protein requires coexpr...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1997-07, Vol.343 (2), p.249-253
Hauptverfasser:	Gerber, N C, Nishida, C R, Ortiz de Montellano, P R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arginine - metabolism Calcium - pharmacology Calmodulin - pharmacology Cloning, Molecular - methods COS Cells Cytochrome c Group - metabolism Egtazic Acid - pharmacology Escherichia coli Humans Isoenzymes - biosynthesis Isoenzymes - metabolism Kinetics Liver - enzymology Nitric Oxide Synthase - chemistry Nitric Oxide Synthase - isolation & purification Nitric Oxide Synthase - metabolism Polymerase Chain Reaction Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Tagged Sites Spectrophotometry
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