Muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism
In the present study we investigated whether muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism as a possible transduction mechanism of contraction. Using isolated bronchial smooth muscle preparations, we found that the muscarinic agonist methacholine cause...
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| Veröffentlicht in: | European journal of pharmacology 1989-05, Vol.164 (2), p.369-371 |
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| container_end_page | 371 |
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| container_issue | 2 |
| container_start_page | 369 |
| container_title | European journal of pharmacology |
| container_volume | 164 |
| creator | Meurs, Herman Timmermans, Adiet Van Amsterdam, Ronald G.M. Brouwer, Frans Kauffman, Henk F. Zaagsma, Johan |
| description | In the present study we investigated whether muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism as a possible transduction mechanism of contraction. Using isolated bronchial smooth muscle preparations, we found that the muscarinic agonist methacholine caused a time- and concentration-dependent accumulation of inositol phosphates in the presence of lithium, an effect which could be inhibited by atropine. Apart from its physiological significance, this finding may have great relevance for the biochemical investigation of cholinergic hyperresponsiveness in the airways of asthmatic patients. |
| doi_str_mv | 10.1016/0014-2999(89)90480-9 |
| format | Article |
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Using isolated bronchial smooth muscle preparations, we found that the muscarinic agonist methacholine caused a time- and concentration-dependent accumulation of inositol phosphates in the presence of lithium, an effect which could be inhibited by atropine. Apart from its physiological significance, this finding may have great relevance for the biochemical investigation of cholinergic hyperresponsiveness in the airways of asthmatic patients.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(89)90480-9</identifier><identifier>PMID: 2547640</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Air breathing ; Atropine - pharmacology ; Biological and medical sciences ; Bronchi - drug effects ; Bronchi - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; lung ; Lung - metabolism ; man ; Methacholine Compounds - pharmacology ; Muscarinic receptors ; Muscle, Smooth - drug effects ; Muscle, Smooth - metabolism ; Phosphatidylinositols - metabolism ; Phosphoinositide metabolism ; phosphoinositides ; Receptors, Muscarinic - drug effects ; Receptors, Muscarinic - metabolism ; Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics ; Smooth muscle (human, airway) ; Time Factors ; Vertebrates: respiratory system</subject><ispartof>European journal of pharmacology, 1989-05, Vol.164 (2), p.369-371</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-90850129c58b04de15243e563ecd2385559b006d88ba73a9d518c6c6f7f226303</citedby><cites>FETCH-LOGICAL-c417t-90850129c58b04de15243e563ecd2385559b006d88ba73a9d518c6c6f7f226303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(89)90480-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6725608$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2547640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meurs, Herman</creatorcontrib><creatorcontrib>Timmermans, Adiet</creatorcontrib><creatorcontrib>Van Amsterdam, Ronald G.M.</creatorcontrib><creatorcontrib>Brouwer, Frans</creatorcontrib><creatorcontrib>Kauffman, Henk F.</creatorcontrib><creatorcontrib>Zaagsma, Johan</creatorcontrib><title>Muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>In the present study we investigated whether muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism as a possible transduction mechanism of contraction. Using isolated bronchial smooth muscle preparations, we found that the muscarinic agonist methacholine caused a time- and concentration-dependent accumulation of inositol phosphates in the presence of lithium, an effect which could be inhibited by atropine. Apart from its physiological significance, this finding may have great relevance for the biochemical investigation of cholinergic hyperresponsiveness in the airways of asthmatic patients.</description><subject>Air breathing</subject><subject>Atropine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bronchi - drug effects</subject><subject>Bronchi - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>lung</subject><subject>Lung - metabolism</subject><subject>man</subject><subject>Methacholine Compounds - pharmacology</subject><subject>Muscarinic receptors</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - metabolism</subject><subject>Phosphatidylinositols - metabolism</subject><subject>Phosphoinositide metabolism</subject><subject>phosphoinositides</subject><subject>Receptors, Muscarinic - drug effects</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics</subject><subject>Smooth muscle (human, airway)</subject><subject>Time Factors</subject><subject>Vertebrates: respiratory system</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEQgIMo6-zqP1DIQWQ9tFbSeV4WZPEFK170KCGdrmYi3Z026Vb235txhjnqoahDffXgK0KeMXjNgKk3AEw03Fp7bewrC8JAYx-QHTPaNqAZf0h2Z-QxuSzlBwBIy-UFueBSaCVgR75_3krwOc4x0IwBlzXlQuNM99vkZ-pj_u3vaZlSWvd0quyI1GekIW3LiD1dE132qdSIcypxjT3SCVffpTGW6Ql5NPix4NNTviLf3r_7evuxufvy4dPt27smCKbXxoKRwLgN0nQgemSSixalajH0vDVSStsBqN6YzuvW214yE1RQgx44Vy20V-Tlce6S088Ny-qmWAKOo58xbcVpy1qpdPtfkElRFyleQXEEQ06lZBzckuPk871j4A763cGtO7h1xrq_-p2tbc9P87duwv7cdPJd6y9OdV-1j0P2c4jljCnNpQJTsZsjhlXar4jZlRBxDtjH-qTV9Sn--44_2OOg9A</recordid><startdate>19890519</startdate><enddate>19890519</enddate><creator>Meurs, Herman</creator><creator>Timmermans, Adiet</creator><creator>Van Amsterdam, Ronald G.M.</creator><creator>Brouwer, Frans</creator><creator>Kauffman, Henk F.</creator><creator>Zaagsma, Johan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890519</creationdate><title>Muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism</title><author>Meurs, Herman ; Timmermans, Adiet ; Van Amsterdam, Ronald G.M. ; Brouwer, Frans ; Kauffman, Henk F. ; Zaagsma, Johan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-90850129c58b04de15243e563ecd2385559b006d88ba73a9d518c6c6f7f226303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Air breathing</topic><topic>Atropine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bronchi - drug effects</topic><topic>Bronchi - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>lung</topic><topic>Lung - metabolism</topic><topic>man</topic><topic>Methacholine Compounds - pharmacology</topic><topic>Muscarinic receptors</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - metabolism</topic><topic>Phosphatidylinositols - metabolism</topic><topic>Phosphoinositide metabolism</topic><topic>phosphoinositides</topic><topic>Receptors, Muscarinic - drug effects</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics</topic><topic>Smooth muscle (human, airway)</topic><topic>Time Factors</topic><topic>Vertebrates: respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meurs, Herman</creatorcontrib><creatorcontrib>Timmermans, Adiet</creatorcontrib><creatorcontrib>Van Amsterdam, Ronald G.M.</creatorcontrib><creatorcontrib>Brouwer, Frans</creatorcontrib><creatorcontrib>Kauffman, Henk F.</creatorcontrib><creatorcontrib>Zaagsma, Johan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meurs, Herman</au><au>Timmermans, Adiet</au><au>Van Amsterdam, Ronald G.M.</au><au>Brouwer, Frans</au><au>Kauffman, Henk F.</au><au>Zaagsma, Johan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1989-05-19</date><risdate>1989</risdate><volume>164</volume><issue>2</issue><spage>369</spage><epage>371</epage><pages>369-371</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>In the present study we investigated whether muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism as a possible transduction mechanism of contraction. Using isolated bronchial smooth muscle preparations, we found that the muscarinic agonist methacholine caused a time- and concentration-dependent accumulation of inositol phosphates in the presence of lithium, an effect which could be inhibited by atropine. Apart from its physiological significance, this finding may have great relevance for the biochemical investigation of cholinergic hyperresponsiveness in the airways of asthmatic patients.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2547640</pmid><doi>10.1016/0014-2999(89)90480-9</doi><tpages>3</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 1989-05, Vol.164 (2), p.369-371 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Air breathing Atropine - pharmacology Biological and medical sciences Bronchi - drug effects Bronchi - metabolism Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques lung Lung - metabolism man Methacholine Compounds - pharmacology Muscarinic receptors Muscle, Smooth - drug effects Muscle, Smooth - metabolism Phosphatidylinositols - metabolism Phosphoinositide metabolism phosphoinositides Receptors, Muscarinic - drug effects Receptors, Muscarinic - metabolism Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics Smooth muscle (human, airway) Time Factors Vertebrates: respiratory system |
| title | Muscarinic receptors in human airway smooth muscle are coupled to phosphoinositide metabolism |
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