Evaluation of time-resolved fluoroimmunoassay with Eu-labelled protein-A for serum progesterone

Progesterone has been assayed in several serum samples by a time-resolved fluoroimmunoassay (TR-FIA). The solid phase was 11α-hydroxyprogesterone hemisuccinate bound covalently to ovalbumin and adsorbed on wells of polystyrene. The assay was based on competitive reaction of solid phase-bound hormone...

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Veröffentlicht in:	Journal of steroid biochemistry 1989-07, Vol.33 (1), p.101-103
Hauptverfasser:	Ius, A., Ferrara, L., Meroni, G., Bacigalupo, M.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Europium Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans Indicators and Reagents Progesterone - blood Staphylococcal Protein A Steroid hormones. Cholecalciferol derivatives Time Factors Vertebrates: endocrinology
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Zusammenfassung:	Progesterone has been assayed in several serum samples by a time-resolved fluoroimmunoassay (TR-FIA). The solid phase was 11α-hydroxyprogesterone hemisuccinate bound covalently to ovalbumin and adsorbed on wells of polystyrene. The assay was based on competitive reaction of solid phase-bound hormone and samples with specific antibody labelled in situ with protein-A prelabelled with europium. The bound Eu was dissociated from the solid phase by an enhancement solution and measured by time-resolved fluorometry. The coefficient of correlation between TR-FIA and RIA was 0.97.
ISSN:	0022-4731
DOI:	10.1016/0022-4731(89)90363-4