IgA Class Antibodies in Dermatitis Herpetiformis: Reaction With Tissue Antigens
The mechanism for deposition of IgA in dermatitis herpetiformis (DH) remains unclear. To test the hypothesis that a circulating IgA class antibody in DH patients binds to constituents of normal human skin, we employed the highly sensitive methods of immunoblotting and indirect immunofluorescence. Se...
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| Veröffentlicht in: | Journal of investigative dermatology 1989-08, Vol.93 (2), p.253-258 |
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| creator | Kadunce, Donald P Meyer, Laurence J Zone, John J |
| description | The mechanism for deposition of IgA in dermatitis herpetiformis (DH) remains unclear. To test the hypothesis that a circulating IgA class antibody in DH patients binds to constituents of normal human skin, we employed the highly sensitive methods of immunoblotting and indirect immunofluorescence. Sera from 64 DH patients, 67 randomly selected normal control subjects, 29 histocompatibility locus antigen (HLA) B8/DR3/DQw2 controls, and 12 psoriatic patients were tested for IgA binding to various substrates, including dermal and epidermal extracts, fibroblast and keratinocyte supernatants, monkey esophagus sections, and whole and saline-split normal human skin sections. Significant differences observed among the groups in the frequency of detectable IgA antibodies reacting with various substrates were as follows: 1) IgA antibodies in 30% of both DH and HLA B8/DR3/DQw2 sera bound to a 60-Kd protein in dermal extracts (p |
| doi_str_mv | 10.1111/1523-1747.ep12277583 |
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To test the hypothesis that a circulating IgA class antibody in DH patients binds to constituents of normal human skin, we employed the highly sensitive methods of immunoblotting and indirect immunofluorescence. Sera from 64 DH patients, 67 randomly selected normal control subjects, 29 histocompatibility locus antigen (HLA) B8/DR3/DQw2 controls, and 12 psoriatic patients were tested for IgA binding to various substrates, including dermal and epidermal extracts, fibroblast and keratinocyte supernatants, monkey esophagus sections, and whole and saline-split normal human skin sections. Significant differences observed among the groups in the frequency of detectable IgA antibodies reacting with various substrates were as follows: 1) IgA antibodies in 30% of both DH and HLA B8/DR3/DQw2 sera bound to a 60-Kd protein in dermal extracts (p<0.25 versus non-HLA matched controls); 2) IgA antiendomysial antibodies were present in 38% of DH patients (predominantly those not on gluten-free diets), whereas both normal control groups had frequencies of 5-10% (p<0.025); 3) there was more nonspecific IgA anti- body-binding to dermal, epidermal, and bovine proteins in DH and HLA control sera than in normal sera; and 4) IgA antibodies directed against the basement membrane were present with an increased frequency of 25% in both DH and HLA B8/DR3/DQw2 sera (p<0.1 versus non-HLA matched controls). Therefore, these results do not support the hypothesis that there is an unique antigen within normal human skin to which IgA antibodies from DH sera bind.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12277583</identifier><identifier>PMID: 2474032</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Aged ; Animals ; Antibodies - immunology ; Antigen-Antibody Reactions ; Antigens - immunology ; Basement membranes ; Biological and medical sciences ; Cells, Cultured ; Child ; Culture Media ; Dermatitis herpetiformis ; Dermatitis Herpetiformis - immunology ; Dermatology ; Epidermal Cells ; Epidermis - immunology ; Esophagus ; Esophagus - immunology ; Female ; Fibroblasts ; Fibroblasts - immunology ; Fluorescent Antibody Technique ; Histocompatibility antigen HLA ; Humans ; Immunoblotting ; Immunofluorescence ; Immunoglobulin A ; Immunoglobulin A - analysis ; Immunoglobulin A - immunology ; Keratinocytes ; Keratins ; Macaca mulatta ; Male ; Medical sciences ; Middle Aged ; Skin ; Skin - cytology ; Skin - immunology ; Skin involvement in other diseases. Miscellaneous. General aspects</subject><ispartof>Journal of investigative dermatology, 1989-08, Vol.93 (2), p.253-258</ispartof><rights>1989 The Society for Investigative Dermatology, Inc</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-89fde83d87b9f701cfc795237a27830abaefca81369c117a45cf9fc13a591c033</citedby><cites>FETCH-LOGICAL-c441t-89fde83d87b9f701cfc795237a27830abaefca81369c117a45cf9fc13a591c033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6706799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2474032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kadunce, Donald P</creatorcontrib><creatorcontrib>Meyer, Laurence J</creatorcontrib><creatorcontrib>Zone, John J</creatorcontrib><title>IgA Class Antibodies in Dermatitis Herpetiformis: Reaction With Tissue Antigens</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>The mechanism for deposition of IgA in dermatitis herpetiformis (DH) remains unclear. To test the hypothesis that a circulating IgA class antibody in DH patients binds to constituents of normal human skin, we employed the highly sensitive methods of immunoblotting and indirect immunofluorescence. Sera from 64 DH patients, 67 randomly selected normal control subjects, 29 histocompatibility locus antigen (HLA) B8/DR3/DQw2 controls, and 12 psoriatic patients were tested for IgA binding to various substrates, including dermal and epidermal extracts, fibroblast and keratinocyte supernatants, monkey esophagus sections, and whole and saline-split normal human skin sections. Significant differences observed among the groups in the frequency of detectable IgA antibodies reacting with various substrates were as follows: 1) IgA antibodies in 30% of both DH and HLA B8/DR3/DQw2 sera bound to a 60-Kd protein in dermal extracts (p<0.25 versus non-HLA matched controls); 2) IgA antiendomysial antibodies were present in 38% of DH patients (predominantly those not on gluten-free diets), whereas both normal control groups had frequencies of 5-10% (p<0.025); 3) there was more nonspecific IgA anti- body-binding to dermal, epidermal, and bovine proteins in DH and HLA control sera than in normal sera; and 4) IgA antibodies directed against the basement membrane were present with an increased frequency of 25% in both DH and HLA B8/DR3/DQw2 sera (p<0.1 versus non-HLA matched controls). Therefore, these results do not support the hypothesis that there is an unique antigen within normal human skin to which IgA antibodies from DH sera bind.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antigen-Antibody Reactions</subject><subject>Antigens - immunology</subject><subject>Basement membranes</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Culture Media</subject><subject>Dermatitis herpetiformis</subject><subject>Dermatitis Herpetiformis - immunology</subject><subject>Dermatology</subject><subject>Epidermal Cells</subject><subject>Epidermis - immunology</subject><subject>Esophagus</subject><subject>Esophagus - immunology</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Fibroblasts - immunology</subject><subject>Fluorescent Antibody Technique</subject><subject>Histocompatibility antigen HLA</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunofluorescence</subject><subject>Immunoglobulin A</subject><subject>Immunoglobulin A - analysis</subject><subject>Immunoglobulin A - immunology</subject><subject>Keratinocytes</subject><subject>Keratins</subject><subject>Macaca mulatta</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Skin</subject><subject>Skin - cytology</subject><subject>Skin - immunology</subject><subject>Skin involvement in other diseases. Miscellaneous. General aspects</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFtr3DAQhUVJSTdp_0ELfgglL041km1ZfSgs20sCCwslpX0TWnmUqnjtjUYu9N9Xe2HzFiEQ6Jwzc_gYewv8BvL5ALWQJahK3eAWhFCqbuULNjt9n7EZ50KUgotfr9gF0R_Ooanq9pydi0pVXIoZW909zItFb4mK-ZDCeuwCUhGG4jPGjU0hBSpuMW4xBT_GTaCPxXe0LoVxKH6G9Lu4D0QT7sMPONBr9tLbnvDN8b1kP75-uV_clsvVt7vFfFm6qoJUttp32MquVWvtFQfnndK5uLJCtZLbtUXvbAuy0Q5A2ap2XnsH0tYaHJfykr0_zN3G8XFCSiZ3c9j3dsBxIqM0CM2VzsbrZ40g2prvb7ZWB6uLI1FEb7YxbGz8Z4CbHXKzY2t2bM0T8hx7d9wwrTfYnUJHxlm_OuqWnO19tIMLdLI1ijdK66cxg01TxJNeNyCF4ln_dNAxY_0bMBpyAQeHXYjokunG8HzP_8X4phM</recordid><startdate>19890801</startdate><enddate>19890801</enddate><creator>Kadunce, Donald P</creator><creator>Meyer, Laurence J</creator><creator>Zone, John J</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19890801</creationdate><title>IgA Class Antibodies in Dermatitis Herpetiformis: Reaction With Tissue Antigens</title><author>Kadunce, Donald P ; Meyer, Laurence J ; Zone, John J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-89fde83d87b9f701cfc795237a27830abaefca81369c117a45cf9fc13a591c033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Antigen-Antibody Reactions</topic><topic>Antigens - immunology</topic><topic>Basement membranes</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Culture Media</topic><topic>Dermatitis herpetiformis</topic><topic>Dermatitis Herpetiformis - immunology</topic><topic>Dermatology</topic><topic>Epidermal Cells</topic><topic>Epidermis - immunology</topic><topic>Esophagus</topic><topic>Esophagus - immunology</topic><topic>Female</topic><topic>Fibroblasts</topic><topic>Fibroblasts - immunology</topic><topic>Fluorescent Antibody Technique</topic><topic>Histocompatibility antigen HLA</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunofluorescence</topic><topic>Immunoglobulin A</topic><topic>Immunoglobulin A - analysis</topic><topic>Immunoglobulin A - immunology</topic><topic>Keratinocytes</topic><topic>Keratins</topic><topic>Macaca mulatta</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Skin</topic><topic>Skin - cytology</topic><topic>Skin - immunology</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kadunce, Donald P</creatorcontrib><creatorcontrib>Meyer, Laurence J</creatorcontrib><creatorcontrib>Zone, John J</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kadunce, Donald P</au><au>Meyer, Laurence J</au><au>Zone, John J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IgA Class Antibodies in Dermatitis Herpetiformis: Reaction With Tissue Antigens</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1989-08-01</date><risdate>1989</risdate><volume>93</volume><issue>2</issue><spage>253</spage><epage>258</epage><pages>253-258</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>The mechanism for deposition of IgA in dermatitis herpetiformis (DH) remains unclear. To test the hypothesis that a circulating IgA class antibody in DH patients binds to constituents of normal human skin, we employed the highly sensitive methods of immunoblotting and indirect immunofluorescence. Sera from 64 DH patients, 67 randomly selected normal control subjects, 29 histocompatibility locus antigen (HLA) B8/DR3/DQw2 controls, and 12 psoriatic patients were tested for IgA binding to various substrates, including dermal and epidermal extracts, fibroblast and keratinocyte supernatants, monkey esophagus sections, and whole and saline-split normal human skin sections. Significant differences observed among the groups in the frequency of detectable IgA antibodies reacting with various substrates were as follows: 1) IgA antibodies in 30% of both DH and HLA B8/DR3/DQw2 sera bound to a 60-Kd protein in dermal extracts (p<0.25 versus non-HLA matched controls); 2) IgA antiendomysial antibodies were present in 38% of DH patients (predominantly those not on gluten-free diets), whereas both normal control groups had frequencies of 5-10% (p<0.025); 3) there was more nonspecific IgA anti- body-binding to dermal, epidermal, and bovine proteins in DH and HLA control sera than in normal sera; and 4) IgA antibodies directed against the basement membrane were present with an increased frequency of 25% in both DH and HLA B8/DR3/DQw2 sera (p<0.1 versus non-HLA matched controls). Therefore, these results do not support the hypothesis that there is an unique antigen within normal human skin to which IgA antibodies from DH sera bind.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>2474032</pmid><doi>10.1111/1523-1747.ep12277583</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adolescent Adult Aged Animals Antibodies - immunology Antigen-Antibody Reactions Antigens - immunology Basement membranes Biological and medical sciences Cells, Cultured Child Culture Media Dermatitis herpetiformis Dermatitis Herpetiformis - immunology Dermatology Epidermal Cells Epidermis - immunology Esophagus Esophagus - immunology Female Fibroblasts Fibroblasts - immunology Fluorescent Antibody Technique Histocompatibility antigen HLA Humans Immunoblotting Immunofluorescence Immunoglobulin A Immunoglobulin A - analysis Immunoglobulin A - immunology Keratinocytes Keratins Macaca mulatta Male Medical sciences Middle Aged Skin Skin - cytology Skin - immunology Skin involvement in other diseases. Miscellaneous. General aspects |
| title | IgA Class Antibodies in Dermatitis Herpetiformis: Reaction With Tissue Antigens |
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