Multicomponent fluorometric analysis using a fiber-optic probe
Single-frequency phase-resolved fluorometry through single and bifurcated fiber-optic probes is used to quantify mixtures of spectrally similar fluorophores. Correlation coefficients from correlation plots are greater than 0.98, and standard errors of estimate are less than 0.13 microM for 80 binary...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1989-07, Vol.61 (14), p.1510-1513 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Bright, Frank V Litwiler, Kevin S |
| description | Single-frequency phase-resolved fluorometry through single and bifurcated fiber-optic probes is used to quantify mixtures of spectrally similar fluorophores. Correlation coefficients from correlation plots are greater than 0.98, and standard errors of estimate are less than 0.13 microM for 80 binary mixtures. Quantification of fluorophores at up to 10:1 molar ratios with lifetime separations of less than 200 ps is possible. The simultaneous quantification of the individual components of ternary and quaternary synthetic mixtures is demonstrated. Application of phase resolution to ambient light rejection is reported also. In this case, the emission spectrum of 10 nM Rhodamine 6G is easily obtained in the presence of a fluctuating, unmodulated background. |
| doi_str_mv | 10.1021/ac00189a010 |
| format | Article |
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Correlation coefficients from correlation plots are greater than 0.98, and standard errors of estimate are less than 0.13 microM for 80 binary mixtures. Quantification of fluorophores at up to 10:1 molar ratios with lifetime separations of less than 200 ps is possible. The simultaneous quantification of the individual components of ternary and quaternary synthetic mixtures is demonstrated. Application of phase resolution to ambient light rejection is reported also. In this case, the emission spectrum of 10 nM Rhodamine 6G is easily obtained in the presence of a fluctuating, unmodulated background.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac00189a010</identifier><identifier>PMID: 2757211</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Anthracenes - analysis ; Chemistry ; Exact sciences and technology ; Fiber Optic Technology ; General, instrumentation ; Optical Fibers ; Rhodamines - analysis ; Spectrometry, Fluorescence - methods</subject><ispartof>Analytical chemistry (Washington), 1989-07, Vol.61 (14), p.1510-1513</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a383t-b33d6a740bbaab42797caf12e3dc015260535e3e67d3e0ba5d54ce566edd4583</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac00189a010$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac00189a010$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6571747$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2757211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bright, Frank V</creatorcontrib><creatorcontrib>Litwiler, Kevin S</creatorcontrib><title>Multicomponent fluorometric analysis using a fiber-optic probe</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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In this case, the emission spectrum of 10 nM Rhodamine 6G is easily obtained in the presence of a fluctuating, unmodulated background.</description><subject>Analytical chemistry</subject><subject>Anthracenes - analysis</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fiber Optic Technology</subject><subject>General, instrumentation</subject><subject>Optical Fibers</subject><subject>Rhodamines - analysis</subject><subject>Spectrometry, Fluorescence - methods</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0LFv1DAUBnCroipHYWJGyoBgqNI-27GdW5BQj_aqtgLBTV2sF-cFuSTxYScS_e8xutOpA5OH7-fn54-xtxzOOQh-gQ6A10sEDkdswZWAUte1eMEWACBLYQBeslcpPWbGgesTdiKMMoLzBft0P_eTd2HYhpHGqej6OcQw0BS9K3DE_in5VMzJjz8LLDrfUCzDNt8otjE09Jodd9gnerM_T9nm6svmcl3efb2-ufx8V6Ks5VQ2UrYaTQVNg9hUwiyNw44Lkq2DvLAGJRVJ0qaVBA2qVlWOlNbUtpWq5Sn7sBubH_09U5rs4JOjvseRwpysWfL8IyEzPNtBF0NKkTq7jX7A-GQ52H9l2WdlZf1uP3ZuBmoPdt9Ozt_vc0wO-y7i6Hw6MK0MN5XJrNwxnyb6c4gx_rLaSKPs5tsP-7C-Xa1Xm5X9nv3HnUeX7GOYY645_XfBv-C3jMY</recordid><startdate>19890715</startdate><enddate>19890715</enddate><creator>Bright, Frank V</creator><creator>Litwiler, Kevin S</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890715</creationdate><title>Multicomponent fluorometric analysis using a fiber-optic probe</title><author>Bright, Frank V ; Litwiler, Kevin S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a383t-b33d6a740bbaab42797caf12e3dc015260535e3e67d3e0ba5d54ce566edd4583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Analytical chemistry</topic><topic>Anthracenes - analysis</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fiber Optic Technology</topic><topic>General, instrumentation</topic><topic>Optical Fibers</topic><topic>Rhodamines - analysis</topic><topic>Spectrometry, Fluorescence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bright, Frank V</creatorcontrib><creatorcontrib>Litwiler, Kevin S</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bright, Frank V</au><au>Litwiler, Kevin S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multicomponent fluorometric analysis using a fiber-optic probe</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 1989-07, Vol.61 (14), p.1510-1513 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79127523 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical chemistry Anthracenes - analysis Chemistry Exact sciences and technology Fiber Optic Technology General, instrumentation Optical Fibers Rhodamines - analysis Spectrometry, Fluorescence - methods |
| title | Multicomponent fluorometric analysis using a fiber-optic probe |
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