Disassembly and reconstitution of yeast 60S ribosomal subunits
Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mumol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different protein...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1989-03, Vol.86 (1), p.55-63 |
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| container_title | Molecular and cellular biochemistry |
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| creator | Nieto, M A Hernández, F Palacián, E |
| description | Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 mumol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits. |
| doi_str_mv | 10.1007/BF00231689 |
| format | Article |
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Treatment with 40 mumol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/BF00231689</identifier><identifier>PMID: 2666849</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Chemical Phenomena ; Chemistry ; Electrophoresis, Polyacrylamide Gel ; Fungal Proteins - isolation & purification ; Fungal Proteins - metabolism ; Hydrogen-Ion Concentration ; Maleic Anhydrides ; Ribosomal Proteins - isolation & purification ; Ribosomal Proteins - metabolism ; RNA, Fungal - analysis ; RNA, Ribosomal - analysis ; Saccharomyces cerevisiae - analysis ; Saccharomyces cerevisiae - metabolism</subject><ispartof>Molecular and cellular biochemistry, 1989-03, Vol.86 (1), p.55-63</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-f72ef724a57cc6fabbb033ad46771d8bc57382e2bc4c0f1badd6342f678907aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2666849$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nieto, M A</creatorcontrib><creatorcontrib>Hernández, F</creatorcontrib><creatorcontrib>Palacián, E</creatorcontrib><title>Disassembly and reconstitution of yeast 60S ribosomal subunits</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. 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| identifier | ISSN: 0300-8177 |
| ispartof | Molecular and cellular biochemistry, 1989-03, Vol.86 (1), p.55-63 |
| issn | 0300-8177 1573-4919 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Chemical Phenomena Chemistry Electrophoresis, Polyacrylamide Gel Fungal Proteins - isolation & purification Fungal Proteins - metabolism Hydrogen-Ion Concentration Maleic Anhydrides Ribosomal Proteins - isolation & purification Ribosomal Proteins - metabolism RNA, Fungal - analysis RNA, Ribosomal - analysis Saccharomyces cerevisiae - analysis Saccharomyces cerevisiae - metabolism |
| title | Disassembly and reconstitution of yeast 60S ribosomal subunits |
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