Enantioselective determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in serum, urine and hair by capillary electrophoresis
A capillary electrophoresis method has been developed for the enantioselective determination of methadone and its main metabolite 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine (EDDP) in serum and urine. After addition of the internal standard diphenhydramine, the serum and urine samples were ext...
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| Veröffentlicht in: | Electrophoresis 1997, Vol.18 (6), p.1026-1034 |
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| creator | Frost, Michael Köhler, Helga Blaschke, Gottfried |
| description | A capillary electrophoresis method has been developed for the enantioselective determination of methadone and its main metabolite 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine (EDDP) in serum and urine. After addition of the internal standard diphenhydramine, the serum and urine samples were extracted at pH 9–10 with n‐hexane. Different cyclodextrins were tested for use as chiral selectors. The best results for simultaneous separation of methadone and EDDP were obtained using heptakis‐(2,6‐di‐O‐methyl)‐β‐cyclodextrin (DIMEB). Data on the accuracy and precision of the method are presented. The method was applied to serum, urine, and hair samples, from individuals undergoing methadone therapy, under consideration of multi‐drug abuse. |
| doi_str_mv | 10.1002/elps.1150180627 |
| format | Article |
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After addition of the internal standard diphenhydramine, the serum and urine samples were extracted at pH 9–10 with n‐hexane. Different cyclodextrins were tested for use as chiral selectors. The best results for simultaneous separation of methadone and EDDP were obtained using heptakis‐(2,6‐di‐O‐methyl)‐β‐cyclodextrin (DIMEB). Data on the accuracy and precision of the method are presented. The method was applied to serum, urine, and hair samples, from individuals undergoing methadone therapy, under consideration of multi‐drug abuse.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.1150180627</identifier><identifier>PMID: 9221894</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>2-Ethylidene-1 ; 2‐Ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidone ; 3-diphenylpyrrolidone ; 5-dimethyl-3 ; Capillary electrophoresis ; Electrophoresis, Capillary - methods ; Enantioselectivity ; Hair - chemistry ; Humans ; Methadone ; Methadone - analysis ; Methadone - blood ; Methadone - urine ; Molecular Conformation ; Pyrrolidines - analysis ; Pyrrolidines - blood ; Pyrrolidines - urine ; Street Drugs - analysis ; Street Drugs - blood ; Street Drugs - urine</subject><ispartof>Electrophoresis, 1997, Vol.18 (6), p.1026-1034</ispartof><rights>Copyright © 1997 VCH Verlagsgesellschaft mbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3827-317c2af1e5f1dd0636deeca9ecde6364290f26d63ce6269c708cae23745df92e3</citedby><cites>FETCH-LOGICAL-c3827-317c2af1e5f1dd0636deeca9ecde6364290f26d63ce6269c708cae23745df92e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.1150180627$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.1150180627$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9221894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frost, Michael</creatorcontrib><creatorcontrib>Köhler, Helga</creatorcontrib><creatorcontrib>Blaschke, Gottfried</creatorcontrib><title>Enantioselective determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in serum, urine and hair by capillary electrophoresis</title><title>Electrophoresis</title><addtitle>ELECTROPHORESIS</addtitle><description>A capillary electrophoresis method has been developed for the enantioselective determination of methadone and its main metabolite 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine (EDDP) in serum and urine. After addition of the internal standard diphenhydramine, the serum and urine samples were extracted at pH 9–10 with n‐hexane. Different cyclodextrins were tested for use as chiral selectors. The best results for simultaneous separation of methadone and EDDP were obtained using heptakis‐(2,6‐di‐O‐methyl)‐β‐cyclodextrin (DIMEB). Data on the accuracy and precision of the method are presented. The method was applied to serum, urine, and hair samples, from individuals undergoing methadone therapy, under consideration of multi‐drug abuse.</description><subject>2-Ethylidene-1</subject><subject>2‐Ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidone</subject><subject>3-diphenylpyrrolidone</subject><subject>5-dimethyl-3</subject><subject>Capillary electrophoresis</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Enantioselectivity</subject><subject>Hair - chemistry</subject><subject>Humans</subject><subject>Methadone</subject><subject>Methadone - analysis</subject><subject>Methadone - blood</subject><subject>Methadone - urine</subject><subject>Molecular Conformation</subject><subject>Pyrrolidines - analysis</subject><subject>Pyrrolidines - blood</subject><subject>Pyrrolidines - urine</subject><subject>Street Drugs - analysis</subject><subject>Street Drugs - blood</subject><subject>Street Drugs - urine</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKtPCmhWSV4hK49Y_SZyoK5hOh0ojaAWIpeWxbzSGxAl2AuS1eMJ6OqOirlhZ995zPh_pIPSK0TNGKT-Hpo9njOWUlbTg8gmasZxzwotSPEUzyqQgtBT5c3Qc43dKaVZl2RE6qjhnZZXN0N-l135wXYQGzOB-AbYwQGid12nrcVfjFoattp0HrL3Fboi41c7v1nrTNW4AzEmSTI2z4IGweU6sa-83RMxFGvot-KnppxCS3rpEeru8vLw5xQkTIYztHI_BHT7YahfwZsJG965pdJjwfbTQ9dsuQHTxBXpW6ybCy8N7gr5eLb8sPpD1p9X14t2aGFFySQSThuuaQV4za2khCgtgdAXGQhoyXtGaF7YQBgpeVEbS0mjgQma5rSsO4gS92XP70P0cIQ6qddFAyuShG6OSFeNciiwJz_dCE7oYA9SqD65NyRWjateS2rWk_rWUHK8P6HHTgn3QH2pJ94v9_bdrYPofTi3XN58f0cne7eIAfx7cOvxQhRQyV98-rlR2e5vRcrVQ78Udmn-yCg</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Frost, Michael</creator><creator>Köhler, Helga</creator><creator>Blaschke, Gottfried</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Enantioselective determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in serum, urine and hair by capillary electrophoresis</title><author>Frost, Michael ; Köhler, Helga ; Blaschke, Gottfried</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3827-317c2af1e5f1dd0636deeca9ecde6364290f26d63ce6269c708cae23745df92e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>2-Ethylidene-1</topic><topic>2‐Ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidone</topic><topic>3-diphenylpyrrolidone</topic><topic>5-dimethyl-3</topic><topic>Capillary electrophoresis</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Enantioselectivity</topic><topic>Hair - chemistry</topic><topic>Humans</topic><topic>Methadone</topic><topic>Methadone - analysis</topic><topic>Methadone - blood</topic><topic>Methadone - urine</topic><topic>Molecular Conformation</topic><topic>Pyrrolidines - analysis</topic><topic>Pyrrolidines - blood</topic><topic>Pyrrolidines - urine</topic><topic>Street Drugs - analysis</topic><topic>Street Drugs - blood</topic><topic>Street Drugs - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frost, Michael</creatorcontrib><creatorcontrib>Köhler, Helga</creatorcontrib><creatorcontrib>Blaschke, Gottfried</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frost, Michael</au><au>Köhler, Helga</au><au>Blaschke, Gottfried</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enantioselective determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in serum, urine and hair by capillary electrophoresis</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>1997</date><risdate>1997</risdate><volume>18</volume><issue>6</issue><spage>1026</spage><epage>1034</epage><pages>1026-1034</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>A capillary electrophoresis method has been developed for the enantioselective determination of methadone and its main metabolite 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine (EDDP) in serum and urine. After addition of the internal standard diphenhydramine, the serum and urine samples were extracted at pH 9–10 with n‐hexane. Different cyclodextrins were tested for use as chiral selectors. The best results for simultaneous separation of methadone and EDDP were obtained using heptakis‐(2,6‐di‐O‐methyl)‐β‐cyclodextrin (DIMEB). Data on the accuracy and precision of the method are presented. The method was applied to serum, urine, and hair samples, from individuals undergoing methadone therapy, under consideration of multi‐drug abuse.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>9221894</pmid><doi>10.1002/elps.1150180627</doi><tpages>9</tpages></addata></record> |
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| ispartof | Electrophoresis, 1997, Vol.18 (6), p.1026-1034 |
| issn | 0173-0835 1522-2683 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 2-Ethylidene-1 2‐Ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidone 3-diphenylpyrrolidone 5-dimethyl-3 Capillary electrophoresis Electrophoresis, Capillary - methods Enantioselectivity Hair - chemistry Humans Methadone Methadone - analysis Methadone - blood Methadone - urine Molecular Conformation Pyrrolidines - analysis Pyrrolidines - blood Pyrrolidines - urine Street Drugs - analysis Street Drugs - blood Street Drugs - urine |
| title | Enantioselective determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in serum, urine and hair by capillary electrophoresis |
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