Substance P and epibatidine-evoked catecholamine release from fractionated chromaffin cells
Bovine chromaffin cells were separated by density gradient centrifugation into subfractions enriched with either >90% adrenaline- or 70–80% noradrenaline-producing cells. Concentrations of epibatidine (an alkaloid with nicotinic receptor activity) as low as 10 nM released adrenaline and noradrena...
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| Veröffentlicht in: | European journal of pharmacology 1997-06, Vol.328 (2), p.249-254 |
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| creator | Krause, Winfried Michael, Norbert Lübke, Carsten Livett, Bruce G Oehme, Peter |
| description | Bovine chromaffin cells were separated by density gradient centrifugation into subfractions enriched with either >90% adrenaline- or 70–80% noradrenaline-producing cells. Concentrations of epibatidine (an alkaloid with nicotinic receptor activity) as low as 10 nM released adrenaline and noradrenaline from both fractions of cells maintained as monolayer cultures. The maximal effect was evoked by 30 nM epibatidine and was comparable to that evoked by 10 μM nicotine. The catecholamine release from the noradrenaline fraction was 30–40% higher than from the adrenaline fraction. Initial exposure to 50 nM epibatidine reduced release induced by a second exposure to the drug. There was cross-desensitization between epibatidine and nicotine. Substance P inhibited the epibatidine-evoked catecholamine release from both fractions by up to 85% (IC
50=3–5 μM). The release of noradrenaline was inhibited more than that of adrenaline. In addition, substance P protected the chromaffin cells against desensitization of the nicotinic receptor by epibatidine. The C-terminal heptapeptide sequence of substance P was 10× less active, two N-terminal sequences did not modulate the catecholamine release. |
| doi_str_mv | 10.1016/S0014-2999(97)83052-X |
| format | Article |
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50=3–5 μM). The release of noradrenaline was inhibited more than that of adrenaline. In addition, substance P protected the chromaffin cells against desensitization of the nicotinic receptor by epibatidine. The C-terminal heptapeptide sequence of substance P was 10× less active, two N-terminal sequences did not modulate the catecholamine release.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/S0014-2999(97)83052-X</identifier><identifier>PMID: 9218708</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adrenal Medulla - cytology ; Adrenaline release ; Adrenals. Interrenals ; Adrenomedullary hormones. Regulation ; Animals ; Biological and medical sciences ; Bridged Bicyclo Compounds, Heterocyclic - pharmacology ; Cattle ; Cell Fractionation ; Cells, Cultured ; Centrifugation, Density Gradient ; Chromaffin cell ; Chromaffin Cells - metabolism ; Epibatidine ; Epinephrine - metabolism ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Nicotinic Agonists - pharmacology ; Noradrenaline release ; Norepinephrine - metabolism ; Pyridines - pharmacology ; Substance P ; Substance P - pharmacology ; Vertebrates: endocrinology</subject><ispartof>European journal of pharmacology, 1997-06, Vol.328 (2), p.249-254</ispartof><rights>1997 Elsevier Science B.V.</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-87b657f771487152f331247ad5a8c26e86f3a7410c9bd6f3044d5e6d53e0befc3</citedby><cites>FETCH-LOGICAL-c455t-87b657f771487152f331247ad5a8c26e86f3a7410c9bd6f3044d5e6d53e0befc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S001429999783052X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2738060$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9218708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krause, Winfried</creatorcontrib><creatorcontrib>Michael, Norbert</creatorcontrib><creatorcontrib>Lübke, Carsten</creatorcontrib><creatorcontrib>Livett, Bruce G</creatorcontrib><creatorcontrib>Oehme, Peter</creatorcontrib><title>Substance P and epibatidine-evoked catecholamine release from fractionated chromaffin cells</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Bovine chromaffin cells were separated by density gradient centrifugation into subfractions enriched with either >90% adrenaline- or 70–80% noradrenaline-producing cells. Concentrations of epibatidine (an alkaloid with nicotinic receptor activity) as low as 10 nM released adrenaline and noradrenaline from both fractions of cells maintained as monolayer cultures. The maximal effect was evoked by 30 nM epibatidine and was comparable to that evoked by 10 μM nicotine. The catecholamine release from the noradrenaline fraction was 30–40% higher than from the adrenaline fraction. Initial exposure to 50 nM epibatidine reduced release induced by a second exposure to the drug. There was cross-desensitization between epibatidine and nicotine. Substance P inhibited the epibatidine-evoked catecholamine release from both fractions by up to 85% (IC
50=3–5 μM). The release of noradrenaline was inhibited more than that of adrenaline. In addition, substance P protected the chromaffin cells against desensitization of the nicotinic receptor by epibatidine. The C-terminal heptapeptide sequence of substance P was 10× less active, two N-terminal sequences did not modulate the catecholamine release.</description><subject>Adrenal Medulla - cytology</subject><subject>Adrenaline release</subject><subject>Adrenals. Interrenals</subject><subject>Adrenomedullary hormones. Regulation</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bridged Bicyclo Compounds, Heterocyclic - pharmacology</subject><subject>Cattle</subject><subject>Cell Fractionation</subject><subject>Cells, Cultured</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromaffin cell</subject><subject>Chromaffin Cells - metabolism</subject><subject>Epibatidine</subject><subject>Epinephrine - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Nicotinic Agonists - pharmacology</subject><subject>Noradrenaline release</subject><subject>Norepinephrine - metabolism</subject><subject>Pyridines - pharmacology</subject><subject>Substance P</subject><subject>Substance P - pharmacology</subject><subject>Vertebrates: endocrinology</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQgIMotT5-QmEPInpYTbKbzeYkUnxBQaEKBQ8hm0xodB812Rb896YPevUyGWa-yQwfQiOCbwgmxe0UY5KnVAhxJfh1mWFG09kBGpKSixRzQg_RcI8co5MQvjDGTFA2QANBI4bLIfqcLqvQq1ZD8pao1iSwcJXqnXEtpLDqvsEkWvWg512tmlhMPNSgAiTWd00MSveuayMRuXksKWtdm2io63CGjqyqA5zv3lP08fjwPn5OJ69PL-P7Sapzxvq05FXBuOWc5CUnjNosIzTnyjBValpAWdhM8ZxgLSoTc5znhkFhWAa4AquzU3S5_Xfhu58lhF42LqwvUC10yyC5IJTgAkeQbUHtuxA8WLnwrlH-VxIs11LlRqpcG5OCy41UOYtzo92CZdWA2U_tLMb-xa6vglZ1lNJqF_YY5Vm5XX-3xSDKWDnwMmgHUb1xHnQvTef-OeQP6lKUTw</recordid><startdate>19970611</startdate><enddate>19970611</enddate><creator>Krause, Winfried</creator><creator>Michael, Norbert</creator><creator>Lübke, Carsten</creator><creator>Livett, Bruce G</creator><creator>Oehme, Peter</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970611</creationdate><title>Substance P and epibatidine-evoked catecholamine release from fractionated chromaffin cells</title><author>Krause, Winfried ; Michael, Norbert ; Lübke, Carsten ; Livett, Bruce G ; Oehme, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-87b657f771487152f331247ad5a8c26e86f3a7410c9bd6f3044d5e6d53e0befc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adrenal Medulla - cytology</topic><topic>Adrenaline release</topic><topic>Adrenals. Interrenals</topic><topic>Adrenomedullary hormones. Regulation</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bridged Bicyclo Compounds, Heterocyclic - pharmacology</topic><topic>Cattle</topic><topic>Cell Fractionation</topic><topic>Cells, Cultured</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromaffin cell</topic><topic>Chromaffin Cells - metabolism</topic><topic>Epibatidine</topic><topic>Epinephrine - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Nicotinic Agonists - pharmacology</topic><topic>Noradrenaline release</topic><topic>Norepinephrine - metabolism</topic><topic>Pyridines - pharmacology</topic><topic>Substance P</topic><topic>Substance P - pharmacology</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krause, Winfried</creatorcontrib><creatorcontrib>Michael, Norbert</creatorcontrib><creatorcontrib>Lübke, Carsten</creatorcontrib><creatorcontrib>Livett, Bruce G</creatorcontrib><creatorcontrib>Oehme, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krause, Winfried</au><au>Michael, Norbert</au><au>Lübke, Carsten</au><au>Livett, Bruce G</au><au>Oehme, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Substance P and epibatidine-evoked catecholamine release from fractionated chromaffin cells</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1997-06-11</date><risdate>1997</risdate><volume>328</volume><issue>2</issue><spage>249</spage><epage>254</epage><pages>249-254</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Bovine chromaffin cells were separated by density gradient centrifugation into subfractions enriched with either >90% adrenaline- or 70–80% noradrenaline-producing cells. Concentrations of epibatidine (an alkaloid with nicotinic receptor activity) as low as 10 nM released adrenaline and noradrenaline from both fractions of cells maintained as monolayer cultures. The maximal effect was evoked by 30 nM epibatidine and was comparable to that evoked by 10 μM nicotine. The catecholamine release from the noradrenaline fraction was 30–40% higher than from the adrenaline fraction. Initial exposure to 50 nM epibatidine reduced release induced by a second exposure to the drug. There was cross-desensitization between epibatidine and nicotine. Substance P inhibited the epibatidine-evoked catecholamine release from both fractions by up to 85% (IC
50=3–5 μM). The release of noradrenaline was inhibited more than that of adrenaline. In addition, substance P protected the chromaffin cells against desensitization of the nicotinic receptor by epibatidine. The C-terminal heptapeptide sequence of substance P was 10× less active, two N-terminal sequences did not modulate the catecholamine release.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9218708</pmid><doi>10.1016/S0014-2999(97)83052-X</doi><tpages>6</tpages></addata></record> |
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| ispartof | European journal of pharmacology, 1997-06, Vol.328 (2), p.249-254 |
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| subjects | Adrenal Medulla - cytology Adrenaline release Adrenals. Interrenals Adrenomedullary hormones. Regulation Animals Biological and medical sciences Bridged Bicyclo Compounds, Heterocyclic - pharmacology Cattle Cell Fractionation Cells, Cultured Centrifugation, Density Gradient Chromaffin cell Chromaffin Cells - metabolism Epibatidine Epinephrine - metabolism Fundamental and applied biological sciences. Psychology In Vitro Techniques Nicotinic Agonists - pharmacology Noradrenaline release Norepinephrine - metabolism Pyridines - pharmacology Substance P Substance P - pharmacology Vertebrates: endocrinology |
| title | Substance P and epibatidine-evoked catecholamine release from fractionated chromaffin cells |
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