Human B cell lines secreting IgM antibody specific for myelin basic protein
In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus (EBV)-transformed peripheral B cells from patients with multiple scle...
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| Veröffentlicht in: | Journal of neuroimmunology 1989-08, Vol.23 (3), p.249-256 |
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| container_title | Journal of neuroimmunology |
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| creator | Jingwu, Zhang Lambrechts, Jos Heyligen, Harry Vandenbark, Arthur A. Raus, Jef |
| description | In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus (EBV)-transformed peripheral B cells from patients with multiple sclerosis precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein (MBP)-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens. |
| doi_str_mv | 10.1016/0165-5728(89)90057-X |
| format | Article |
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The technique based on limiting dilutions of Epstein-Barr virus (EBV)-transformed peripheral B cells from patients with multiple sclerosis precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein (MBP)-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. 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The technique based on limiting dilutions of Epstein-Barr virus (EBV)-transformed peripheral B cells from patients with multiple sclerosis precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein (MBP)-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.</description><subject>Antibodies, Anti-Idiotypic - immunology</subject><subject>Antibody Specificity</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle</subject><subject>Cell Line, Transformed</subject><subject>Epstein-Barr virus transformation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Herpesvirus 4, Human</subject><subject>Humans</subject><subject>IgM-secreting B lymphoblastoid cell line</subject><subject>Immunobiology</subject><subject>Immunoglobulin M - immunology</subject><subject>Immunological reactions in vitro</subject><subject>Lymphoid cell lines. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Herpesvirus 4, Human</topic><topic>Humans</topic><topic>IgM-secreting B lymphoblastoid cell line</topic><topic>Immunobiology</topic><topic>Immunoglobulin M - immunology</topic><topic>Immunological reactions in vitro</topic><topic>Lymphoid cell lines. Hybrids</topic><topic>Myelin basic protein</topic><topic>Myelin Basic Protein - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jingwu, Zhang</creatorcontrib><creatorcontrib>Lambrechts, Jos</creatorcontrib><creatorcontrib>Heyligen, Harry</creatorcontrib><creatorcontrib>Vandenbark, Arthur A.</creatorcontrib><creatorcontrib>Raus, Jef</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jingwu, Zhang</au><au>Lambrechts, Jos</au><au>Heyligen, Harry</au><au>Vandenbark, Arthur A.</au><au>Raus, Jef</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human B cell lines secreting IgM antibody specific for myelin basic protein</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1989-08-01</date><risdate>1989</risdate><volume>23</volume><issue>3</issue><spage>249</spage><epage>256</epage><pages>249-256</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><coden>JNRIDW</coden><abstract>In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus (EBV)-transformed peripheral B cells from patients with multiple sclerosis precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5–12 μg/ml antibody after 2 weeks in culture. The myelin basic protein (MBP)-specific B cells were surface IgM positive, and occured with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2473999</pmid><doi>10.1016/0165-5728(89)90057-X</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 1989-08, Vol.23 (3), p.249-256 |
| issn | 0165-5728 1872-8421 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antibodies, Anti-Idiotypic - immunology Antibody Specificity B-Lymphocytes - immunology Biological and medical sciences Cell Cycle Cell Line, Transformed Epstein-Barr virus transformation Fundamental and applied biological sciences. Psychology Fundamental immunology Herpesvirus 4, Human Humans IgM-secreting B lymphoblastoid cell line Immunobiology Immunoglobulin M - immunology Immunological reactions in vitro Lymphoid cell lines. Hybrids Myelin basic protein Myelin Basic Protein - immunology |
| title | Human B cell lines secreting IgM antibody specific for myelin basic protein |
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